609 research outputs found

    Monitoramento de Sinoxylon conigerum (Gerstäcker, 1885) (Coleoptera: Bostrichidae) em madeira de teca (Tectona grandis L. f.) no Estado do Pará.

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    Estudo de algumas características fenotípicas relacionadas ao desempenho de tourinhos da raça Canchim em confinamento: 1. Desenvolvimento ponderal e polimorfismo para cor da mufla.

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    O objetivo do trabalho foi avaliar uma possivel associação entre polimorfismo para cor da mufla e desempenho de tourinhos Canchim. O trabalho foi realizado em dois locais diferentes. Na Fazenda São Jorge (Cedral-SP), foram utilizados 51 aimais inteiros da raça Canchim com médias de 20 meses de idade e 327 kg de peso vivo ao inicio do período, e classificados em 3 grupos em relação a cor de mufla (escura, rosada e pigmentação intermediaria). O mesmo procedimento for feito para 105 animais que estavam participando da prova de Ganho de Peso na Estação Experimental de Zootecnia de Sertãozinho (SP), iniciando a prova com 210 dias e terminando com 378 dias de idade. Os resultados mostraram que não existiu associação significativa entre os grupos com diferentes cores de mucosa e o ganho diário em regime de confinamento.Resumo expandido. Publicado tambem por In: Reuniao Anual da Sociedade Brasileira de Zootecnia, 33, 1996, Fortaleza. Anais... Fortaleza: SBZ, 1996, v.1 p.158-160

    Environment Impact of Moxidectin in Sheep Faeces upon Dung Diptera in South of Brazil

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    Dung Diptera can be beneficial to the environment because the adults feed on, deposit eggs in, and the larval nutrition depends on the feaces of animals which significantly im-proves nutrient cycling. However, anthelmintics which are used to control internal parasites have long persistence with a long half-life in faeces. This is harmful to the coprofauna and may adversely affect the diversity of this environmen-tal system. In this study we evaluated the toxicity of moxidectin anthelmintic on the dung Diptera population in pastures of South of Brazil

    A Paracoccidioides brasiliensis glycan shares serologic and functional properties with cryptococcal glucuronoxylomannan

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    The cell wall of the yeast form of the dimorphic fungus Paracoccidioides brasiliensis is enriched with alpha 1,3-glucans. in Cryptococcus neoformans, alpha 1,3-glucans interact with glucuronoxylomannan (GXM), a hetero-polysaccharide that is essential for fungal virulence. in this study, we investigated the occurrence of P. brasiliensis glycans sharing properties with cryptococcal GXM. Protein database searches in P. brasiliensis revealed the presence of sequences homologous to those coding for enzymes involved in the synthesis of GXM and capsular architecture in C. neoformans. in addition, monoclonal antibodies (mAbs) raised to cryptococcal GXM bound to P. brasiliensis cells. Using protocols that were previously established for extraction and analysis of C neoformans GXM, we recovered a P. brasiliensis glycan fraction composed of mannose and galactose, in addition to small amounts of glucose, xylose and rhamnose. in comparison with the C. neoformans GXM, the P. brasiliensis glycan fraction components had smaller molecular dimensions. the P. brasiliensis components, nevertheless, reacted with different GXM-binding mAbs. Extracellular vesicle fractions of P. brasiliensis also reacted with a GXM-binding mAb, suggesting that the polysaccharide-like molecule is exported to the extracellular space in secretory vesicles. An acapsular mutant of C. neoformans incorporated molecules from the P. brasiliensis extract onto the cell wall, resulting in the formation of surface networks that resembled the cryptococcal capsule. Coating the C. neoformans acapsular mutant with the P. brasiliensis glycan fraction resulted in protection against phagocytosis by murine macrophages. These results suggest that P. brasiliensis and C. neoformans share metabolic pathways required for the synthesis of similar polysaccharides and that P. brasiliensis yeast cell walls have molecules that mimic certain aspects of C. neoformans GXM. These findings are important because they provide additional evidence for the sharing of antigenically similar components across phylogenetically distant fungal species. Since GXM has been shown to be important for the pathogenesis of C neoformans and to elicit protective antibodies, the finding of similar molecules in P. brasiliensis raises the possibility that these glycans play similar functions in paracoccidiomycosis. (C) 2012 Elsevier Inc. All rights reserved.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Fundação de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ)NIHCenter for AIDS Research at EinsteinInterhemispheric Research Training Grant in Infectious Diseases, Fogarty International CenterDepartment of EnergyFiocruz MS, CDTS, BR-21040360 Rio de Janeiro, BrazilUniv Fed Rio de Janeiro, Inst Microbiol Prof Paulo de Goes, BR-21941902 Rio de Janeiro, BrazilAlbert Einstein Coll Med, Dept Microbiol & Immunol, Bronx, NY 10461 USAUniversidade Federal de São Paulo, Disciplina Biol Celular, BR-04023062 São Paulo, BrazilUniv Fed Rio de Janeiro, Inst Biofis Carlos Chagas Filho, Lab Ultraestrutura Celular Hertha Meyer, BR-21941903 Rio de Janeiro, BrazilAlbert Einstein Coll Med, Div Infect Dis, Dept Med, Bronx, NY 10461 USAUniversidade Federal de São Paulo, Disciplina Biol Celular, BR-04023062 São Paulo, BrazilNIH: AI033142NIH: AI033774NIH: AI052733NIH: HL059842Interhemispheric Research Training Grant in Infectious Diseases, Fogarty International Center: NIH D43-TW007129Department of Energy: DE-FG-9-93ER-20097Web of Scienc
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