Synthesis and antihyperlipidemic activity of Dibenz[c,e]azepine-5,7-dione derivatives in Triton WR-1339-induced hyperlipidemic rats

The structural requirements for decreasing cholesterol and triglyceride levels remain largely uninvestigated. Thus a systematic investigation of certain N-substituted dibenz[c,e]azepines is necessary. Nine compounds of N-substituted derivatives of Dibenz[c,e]azepine-5,7-Dione were synthesized and they were tested for antihyperlipidemic activity. Compounds synthesized (C-1, C-3, C-4 and C-5) significantly lowered the serum total cholesterol, triglyceride and LDL (low density lipoprotein) levels and also improved the HDL (high density lipoprotein) level in Triton-WR 1339 induced hyperlipidemic rats. Keywords: antihyperlipidemic activity, lipids in plasma, high density lipoprotei

EVALUATION OF IN VITRO ANTI-INFLAMMATORY ACTIVITY AND HPTLC ANALYSIS OF PLANT PHYLLANTHUS FRATERNUS

Objective: The present investigation evaluated in vitro anti-inflammatory activity of Phyllanthus fraternus. Inhibition of Cyclooxygenase and 5-lipoxygenase was performed along with protein denaturation.Methods: Alcoholic extract of plant was subjected to in vitro anti-inflammatory activity and HPTLC analysis.Results: The results of anti-inflammatory activity showed significant inhibition in Cyclooxygenase and 5-lipoxygenase assay, extract also showed more than 70 % inhibition in protein denaturation method. HPTLC of plant materials was also performed; spots of alkaloids were recorded.Conclusion: Different alkaloids were spotted in chromatographic analysis and study suggested that anti-inflammatory activity of Phyllanthus fraternus may be due to the presence of alkaloids.Â

ANTIMICROBIAL AND PHYTOCHEMICAL EVALUATION OF CISSUS QUADRANGULARIS L.

The present investigation involves antimicrobial and phytochemical evaluation of Cissus quadrangularis L. The antibacterial activity of Cissus quadrangularis was performed using disk diffusion method. The Results of study proved prompt efficacy of herbal extract against S. aureus and E. coli. The concentration dependent antibacterial activity of extract was observed against both organisms. Study also involves phytochemical investigation of herbal extract using HPTLC, IR and UV-Visible spectrophotometer. The result of study indicated that the methanolic extract possessed most potent antibacterial activity as compared to other extract. The antibacterial activity increases with the concentration and results indicated that the diameters of zone inhibition of the extract were comparable with the standard drug. The antimicrobial potential of plant extract may be attributed to the presence of specific phytoconstituents

Phytochemical and Anti-Inflammatory Evaluation of Herbal Gel Prepared from Bark Extract of Mesua Ferrea Linn

The present research work involves formulation of herbal gel containing stem bark extracts of Mesua ferrea Linn and its evaluation for in vitro anti-inflammatory activity. The gel formulations were prepared using ethanolic extracts along with different polymer. The physiochemical parameters of formulations like; pH, viscosity and spreadability etc. also evaluated. Phytochemical analysis revealed presence of phenols, flavonoids and alkaloids, etc. The ethanolic extracts showed appreciable anti-inflammatory activity compared to the standard drug. Study confirmed that potential anti-inflammatory formulation can be developed from bark extract of Mesua ferrea Linn.   Keywords: Mesua ferrea Linn., Anti-inflammatory, Herbal Gel, Phytochemical, Albumin denaturation

PHYTOCHEMICAL STANDARDIZATION AND ANTIOXIDANT POTENTIAL OF AYURVEDA FORMULATION DARVYADI RASKRIYA

The study represents phytochemical standardization and antioxidant potential of ayurveda formulation Darvyadi Raskriya. The study involves development of high-performance thin-layer chromatographic (HPTLC) method for analysis of formulation. The study utilizes analysis of glycyrrhizin in formulation which is the phytoconstituent of Glycyrrhiza glabra one of the component of formulation. The sample in ethanol was applied on aluminium TLC plates using Linomat 5 spray (CAMAG). Linear ascending development was performed in twin trough glass chamber saturated with mobile phase. The mobile phase consisted of ethyl acetate-methanol-formic acid (10:5:1 v/v/v). The spectrodensitometric detection was performed at the wavelength of 254 nm. The regression analysis was found to be linear with r2=0.997 in the concentration range 5-25 ppm. The antioxidant activity of formulation was also found to be significant as compared to control. Keywords: Standardization, Glycyrrhiza glabra, Glycyrrhizin, HPTLC, Antioxidant

Transcriptome analysis reveals immune pathways underlying resistance in the common carp Cyprinus carpio against the oomycete Aphanomyces invadans

Infection with Aphanomyces invadans is a serious fish disease with major global impacts. Despite affecting over 160 fish species, some of the species like the common carp Cyprinus carpio are resistant to A. invadans infection. In the present study, we investigated the transcriptomes of head kidney of common carp experimentally infected with A. invadans. In time course analysis, 5288 genes were found to be differentially expressed (DEGs), of which 731 were involved in 21 immune pathways. The analysis of immune-related DEGs suggested that efficient processing and presentation of A. invadans antigens, enhanced phagocytosis, recognition of pathogen-associated molecular patterns, and increased recruitment of leukocytes to the sites of infection contribute to resistance of common carp against A. invadans. Herein, we provide a systematic understanding of the disease resistance mechanisms in common carp at molecular level as a valuable resource for developing disease management strategies for this devastating fish-pathogenic oomycete

Cytogenetic studies of fish species Horabagrus nigricollaris, Puntius denisonii and Puntius sarana subnasutus endemic to the Western Ghats

Cytogenetic studies of fish species Horabagrus nigricollaris, Puntius denisonii and Puntius sarana subnasutus endemic to the Western Ghat

The CMS Outer Hadron Calorimeter

The CMS hadron calorimeter is a sampling calorimeter with brass absorber and plastic scintillator tiles with wavelength shifting fibres for carrying the light to the readout device. The barrel hadron calorimeter is complemented with a outer calorimeter to ensure high energy shower containment in CMS and thus working as a tail catcher. Fabrication, testing and calibrations of the outer hadron calorimeter are carried out keeping in mind its importance in the energy measurement of jets in view of linearity and resolution. It will provide a net improvement in missing \et measurements at LHC energies. The outer hadron calorimeter has a very good signal to background ratio even for a minimum ionising particle and can hence be used in coincidence with the Resistive Plate Chambers of the CMS detector for the muon trigger

Constraints on the χ_(c1) versus χ_(c2) polarizations in proton-proton collisions at √s = 8 TeV

The polarizations of promptly produced χ_(c1) and χ_(c2) mesons are studied using data collected by the CMS experiment at the LHC, in proton-proton collisions at √s=8  TeV. The χ_c states are reconstructed via their radiative decays χ_c → J/ψγ, with the photons being measured through conversions to e⁺e⁻, which allows the two states to be well resolved. The polarizations are measured in the helicity frame, through the analysis of the χ_(c2) to χ_(c1) yield ratio as a function of the polar or azimuthal angle of the positive muon emitted in the J/ψ → μ⁺μ⁻ decay, in three bins of J/ψ transverse momentum. While no differences are seen between the two states in terms of azimuthal decay angle distributions, they are observed to have significantly different polar anisotropies. The measurement favors a scenario where at least one of the two states is strongly polarized along the helicity quantization axis, in agreement with nonrelativistic quantum chromodynamics predictions. This is the first measurement of significantly polarized quarkonia produced at high transverse momentum