27 research outputs found

    Process Oriented Dependency Modelling for Service Identification

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    Part 3: Finance and Service ScienceInternational audienceService-Oriented Architecture (SOA) is important for organisations to achieve dynamic business process and build business agility. One of the first step for service oriented applications implementation is to properly identify a set of fine-grained services. A right service granularity is necessary to satisfy lower coupling and higher cohesion principles for reusable software services. To meet this challenge, a lot of efforts have been attached to support service identification. In this paper, by considering the dependency combined with the idea of graph partition, a service identification method is proposed from the business process’s perspective. The illustration example has shown its promising and it is expected that the proposed service identification method can offer researchers further insight into service granularity analysis

    Personality Traits among Professional Students with Special Reference to Self- Motivation

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    Abstract This paper makes an attempt on Personality traits among the professional students with special reference to Self-Motivatio

    Construction and characterization of Drosophila ether-a-go-go-related gene potassium channel-t2a-gal4 expression plasmid for analysis of transcriptional gene regulation

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    The ether-a-go-go-related gene (ERG) encodes for a voltage-dependent potassium channel expressed in the nervous and cardiac systems and is one of several causes for familial forms of Long QT syndrome. Traditional strategies for the analysis of gene expression in Drosophila melanogaster have utilized P-element-based expression plasmid constructs such as pPTGAL4. Previously, a Drosophila ERG potassium channel gene putative promoter was cloned into pPTGAL4 and transgenic flies were examined for expression of a green fluorescent protein reporter. This work yielded variable results, which at best were inconclusive. An alternate approach was attempted by construction of a hybrid DERG-T2A-GAL4-based expression construct that is in turn cloned into pBID, a site-specific integration plasmid with insulators to prevent external regulatory sequences influencing expression of the reporter gene. The assembled plasmid will drive expression of a DERG-T2A-GAL4 fusion protein that can undergo proteolysis at the T2A site and thus allow GAL4 to mobilize into nuclear compartment and drive expression of reporter genes, including fluorescent proteins or enzymes. The assembly of this plasmid will facilitate the analysis of gene promoter activity for the DERG gene in an insulated background with a well-defined genomic location. This will provide a tool in the analysis of gene activity for the DERG potassium channel of Drosophila melanogaster. Additionally, the Drosophila down and out (dao) gene, which codes for a DERG accessory subunit, was modified via the plug-and-play insertion of GAL4 into the dao gene. This approach will provide a genetic line that will permit simultaneous comparison of DERG and dao expression and provide evidence of coexpression of the two subunits and shed possible roles of dao in regulation or expression of other potassium channels
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