Lens mounting techniques for precise radial location of fragile lenses in the NGS2 and Veloce instruments

We present novel methods for mounting lenses in a pair of instruments that presented challenging optical and mechanical requirements. The first instrument is the replacement Natural Guide Star Sensor (NGS2) for CANOPUS at Gemini South, which incorporates an objective consisting of a stack of six lenses mounted in a common bore. A compliant radial spacer was used to eliminate lens decentre resulting from the additional radial clearance required to accommodate differential thermal strains between the low thermal expansion lenses and a common bore. In the same instrument, tangent contact toroidal spacers were deployed in place of traditional conical spacers to further reduce contact stresses in fragile calcium fluoride lens elements. The toroidal faces were specified with a 10µm profile tolerance to avoid possible edge contact between the spacers and lenses. We investigated milling and turning machining processes for the production of the spacers by comparing their results via Coordinate Measuring Machine (CMM) measurements. In the second instrument, Veloce, built for the Anglo-Australian Telescope, a lens decentre requirement of 40µm led us to develop a simple means of in-situ centring adjustment of the cell mounted lens. Physical testing of the finished instruments verified the performance of each of these methods. NGS2 produced images at the factory acceptance test in which 94% of encircled energy was captured by a single 16um detector pixel, surpassing the specification of 80%. Bench testing of Veloce during assembly showed that the adjustment mechanism allowed centring of the lens over a range of +/- 0.1mm with a precision of 5µm

AO corrected satellite imaging from Mount Stromlo

The Research School of Astronomy and Astrophysics have been developing adaptive optics systems for space situational awareness. As part of this program we have developed satellite imaging using compact adaptive optics systems for small (1-2 m) telescopes such as those operated by Electro Optic Systems (EOS) from the Mount Stromlo Observatory. We have focused on making compact, simple, and high performance AO systems using modern high stroke high speed deformable mirrors and EMCCD cameras. We are able to track satellites down to magnitude 10 with a Strehl in excess of 20% in median seeing

Pyxis: A ground-based demonstrator for formation-flying optical interferometry

In the past few years, there has been a resurgence in studies towards space-based optical/infrared interferometry, particularly with the vision to use the technique to discover and characterise temperate Earth-like exoplanets around solar analogues. One of the key technological leaps needed to make such a mission feasible is demonstrating that formation flying precision at the level needed for interferometry is possible. Here, we present $\textit{Pyxis}$, a ground-based demonstrator for a future small satellite mission with the aim to demonstrate the precision metrology needed for space-based interferometry. We describe the science potential of such a ground-based instrument, and detail the various subsystems: three six-axis robots, a multi-stage metrology system, an integrated optics beam combiner and the control systems required for the necessary precision and stability. We end by looking towards the next stage of $\textit{Pyxis}$: a collection of small satellites in Earth orbit.Comment: 27 Pages, 14 Figures, submitted to JATI

Anomalous scattering analysis of Agrobacterium radiobacter phosphotriesterase: the prominent role of iron in the heterobinuclear active site

Bacterial phosphotriesterases are binuclear metalloproteins from which the catalytic mechanism has been studied with a variety of techniques, principally using active sites reconstituted in vitro from apo-enzymes. Here, atomic absorption spectroscopy and anomalous X-ray scattering and have been used to determine the identity of the metals incorporated into the active site in vivo. We have recombinantly expressed the phosphotriesterase from Agrobacterium radiobacter (OpdA) in Escherichia coli grown in medium supplemented with 1 mM CoCl2, and in unsupplemented medium. Anomalous scattering data, collected from a single crystal at the Fe-K, Co-K and Zn-K edges, indicate that iron and cobalt are the primary constituents of the two metal binding sites in the catalytic centre ( and ), in protein expressed in E. coli grown in supplemented medium. Comparison to OpdA expressed in unsupplemented medium demonstrates that the cobalt present in the supplemented medium replaced zinc at the -position of the active site, which results in an increase in the catalytic efficiency of the enzyme. These results suggest an essential role for iron in the catalytic mechanism of bacterial phosphotriesterases, and that they are natively heterobinuclear iron-zinc enzymes

Probing the role of the divalent metal ion in uteroferrin using metal ion replacement and a comparison to isostructural biomimetics

Purple acid phosphatases (PAPs) are a group of heterovalent binuclear metalloenzymes that catalyze the hydrolysis of phosphomonoesters at acidic to neutral pH. While the metal ions are essential for catalysis, their precise roles are not fully understood. Here, the Fe(III)Ni(II) derivative of pig PAP (uteroferrin) was generated and its properties were compared with those of the native Fe(III)Fe(II) enzyme. The kcat of the Fe(III)Ni(II) derivative (approximately 60 s–1) is approximately 20% of that of native uteroferrin, and the Ni(II) uptake is considerably faster than the reconstitution of full enzymatic activity, suggesting a slow conformational change is required to attain optimal reactivity. An analysis of the pH dependence of the catalytic properties of Fe(III)Ni(II) uteroferrin indicates that the l-hydroxide is the likely nucleophile. Thus, the Ni(II) derivative employs a mechanism similar to that proposed for the Ga(III)Zn(II) derivative of uteroferrin, but different from that of the native enzyme, which uses a terminal Fe(III)-bound nucleophile to initiate catalysis. Binuclear Fe(III)Ni(II) biomimetics with coordination environments similar to the coordination environment of uteroferrin were generated to provide both experimental benchmarks (structural and spectroscopic) and further insight into the catalytic mechanism of hydrolysis. The data are consistent with a reaction mechanism employing an Fe(III)-bound terminal hydroxide as a nucleophile, similar to that proposed for native uteroferrin and various related isostructural biomimetics. Thus, only in the uteroferrin- catalyzed reaction are the precise details of the catalytic mechanism sensitive to the metal ion composition, illustrating the significance of the dynamic ligand environment in the protein active site for the optimization of the catalytic efficiency

A conceptual design study for Subaru ULTIMATE GLAO

We report on the conceptual design study done for the Ground Layer Adaptive Optics system of the ULTIMATE-Subaru project. This is an ambitious instrument project, providing GLAO correction in a square field of view of 14 arcmin on a side, aiming to deliver improved seeing at the near infrared wavelength. Its client instruments are an imager and multi-IFU spectrograph at Cassegrain and a Multi-Object spectrograph at Nasmyth. In this paper, we introduce the ULTIMATE-Subaru project overview and its science case and report the results of the GLAO performance prediction based on the numerical simulation and conceptual design of the wavefront sensor systemThe development of ULTIMATE-Subaru is partly supported by the Japan Society for the Promotion of Science (Grant-in-Aid for Research #17H06129)

Does owning a pet protect older people against loneliness?

This article has been made available through the Brunel Open Access Publishing Fund.Pet ownership is thought to make a positive contribution to health, health behaviours and the general well-being of older people. More specifically pet ownership is often proposed as a solution to the problem of loneliness in later life and specific 'pet based' interventions have been developed to combat loneliness. However the evidence to support this relationship is slim and it is assumed that pet ownership is a protection against loneliness rather than a response to loneliness. The aim of this paper is to examine the association between pet ownership and loneliness by exploring if pet ownership is a response to, or protection against, loneliness using Waves 0-5 from the English Longitudinal Study of Ageing (ELSA)