OH-selected AGB and post-AGB objects I.Infrared and maser properties

Using 766 compact objects from a survey of the galactic Plane in the 1612-MHz OH line, new light is cast on the infrared properties of evolved stars on the TP-AGB and beyond. The usual mid-infrared selection criteria, based on IRAS colours, largely fail to distinguish early post-AGB stages. A two-colour diagram from narrower-band MSX flux densities, with bimodal distributions, provides a better tool to do the latter. Four mutually consistent selection criteria for OH-masing red PPNe are given, as well as two for early post-AGB masers and one for all post--AGB masers, including the earliest ones. All these criteria miss a group of blue, high-outflow post-AGB sources with 60-mum excess; these will be discussed in detail in Paper II. The majority of post-AGB sources show regular double-peaked spectra in the OH 1612-MHz line, with fairly low outflow velocities, although the fractions of single peaks and irregular spectra may vary with age and mass. The OH flux density shows a fairly regular relation with the stellar flux and the envelope optical depth, with the maser efficiency increasing with IRAS colour R21. The OH flux density is linearly correlated with the 60-mum flux density.Comment: 16 pages, LaTex, 22 figures, AJ (accepted

An Attempt to Detect the Galactic Bulge at 12 microns with IRAS

Surface brightness maps at 12 microns, derived from observations with the Infrared Astronomical Satellite (IRAS), are used to estimate the integrated flux at this wavelength from the Galactic bulge as a function of galactic latitude along the minor axis. A simple model was used to remove Galactic disk emission (e.g. unresolved stars and dust) from the IRAS measurements. The resulting estimates are compared with predictions for the 12 micron bulge surface brightness based on observations of complete samples of optically identified M giants in several minor axis bulge fields. No evidence is found for any significant component of 12m emission in the bulge other than that expected from the optically identified M star sample plus normal, lower luminosity stars. Known large amplitude variables and point sources from the IRAS catalogue contribute only a small fraction to the total 12 micron flux.Comment: Accepted for publication in ApJ; 13 pages of text including tables in MS WORD97 generated postscript; 3 figures in postscript by Sigma Plo

HST Snapshot Survey of Post-AGB Objects

The results from a Hubble Space Telescope (HST) snapshot survey of post-AGB objects are shown. The aim of the survey is to complement existing HST images of PPN and to connect various types of nebulosities with physical and chemical properties of their central stars. Nebulosities are detected in 15 of 33 sources. Images and photometric and geometric measurements are presented. For sources with nebulosities we see a morphological bifurcation into two groups, DUPLEX and SOLE, as previous studies have found. We find further support to the previous results suggesting that this dichotomy is caused by a difference in optical thickness of the dust shell. The remaining 18 sources are classified as stellar post-AGB objects, because our observations indicate a lack of nebulosity. We show that some stellar sources may in fact be DUPLEX or SOLE based on their infrared colors. The cause of the differences among the groups are investigated. We discuss some evidence suggesting that high progenitor-mass AGB stars tend to become DUPLEX post-AGB objects. Intermediate progenitor-mass AGB stars tend to be SOLE post-AGB objects. Most of the stellar sources probably have low mass progenitors and do not seem to develop nebulosities during the post-AGB phase and therefore do not become planetary nebulae.Comment: 21 pages, 11 figure

Proton Motive Force-Dependent Hoechst 33342 Transport by the ABC Transporter LmrA of Lactococcus lactis

The fluorescent compound Hoechst 33342 is a substrate for many multidrug resistance (MDR) transporters and is widely used to characterize their transport activity. We have constructed mutants of the adenosine triphosphate (ATP) binding cassette (ABC)-type MDR transporter LmrA of Lactococcus lactis that are defective in ATP hydrolysis. These mutants and wild-type LmrA exhibited an atypical behavior in the Hoechst 33342 transport assay. In membrane vesicles, Hoechst 33342 transport was shown to be independent of the ATPase activity of LmrA, and it was not inhibited by orthovanadate but sensitive to uncouplers that collapse the proton gradient and to N,N'-dicyclohexylcarbodiimide, an inhibitor of the F0F1-ATPase. In contrast, transport of Hoechst 33342 by the homologous, heterodimeric MDR transporter LmrCD showed a normal ATP dependence and was insensitive to uncouplers of the proton gradient. With intact cells, expression of LmrA resulted in an increased rate of Hoechst 33342 influx while LmrCD caused a decrease in the rate of Hoechst 33342 influx. Cellular toxicity assays using a triple knockout strain, i.e., L. lactis ΔlmrA ΔlmrCD, demonstrate that expression of LmrCD protects cells against the growth inhibitory effects of Hoechst 33342, while in the presence of LmrA, cells are more susceptible to Hoechst 33342. Our data demonstrate that the LmrA-mediated Hoechst 33342 transport in membrane vesicles is influenced by the transmembrane pH gradient due to a pH-dependent partitioning of Hoechst 33342 into the membrane.

Anterolateral proximal tibial opening wedge osteotomy for biplanar correction in genu valgum recurvatum using patient specific instrumentation (PSI):A technical note

BACKGROUND: Biplanar anterolateral proximal tibial opening wedge osteotomy has been described as successful for patients with combined recurvatum and valgus alignment. As it is a correction in two planes, it is a technically demanding procedure. We report the use of a novel technique with patient specific instrumentation (PSI) guides for different steps, aiming to reduce the complexity of this procedure. METHODS: One patient was treated for genu valgum recurvatum. A virtual surgical plan was made. A two-step PSI approach was used, consisting of an osteotomy guide and a repositioning guide and a custom trial wedges. RESULTS: Follow-up showed full function and improved VAS and KOOS scores. A neutral alignment was achieved. There was 2.76 degrees less varus compared to pre-operative planning, 1.24 degrees of excess slope and a rotational difference of 0.10 degrees. Saw plane accuracy was within 1 mm. CONCLUSIONS: PSI is a recent technical addition to HTOs as a modality to improve accuracy and reduce surgical complexity. Pre-planning and PSI use in an anterolateral opening wedge PTO allowed for an accurate and reproducible biplanar correction in genu valgum recurvatum. Accuracy was comparable to PSI use in lateral open wedge high tibial osteotomies

Webteaching: sequencing of subject matter in relation to prior knowledge of pupils

Two experiments are discussed in which the sequencing procedure of webteaching is compared with a linear sequence for the presentation of text material.\ud \ud In the first experiment variations in the level of prior knowledge of pupils were studied for their influence on the sequencing mode of text presentation. Prior knowledge greatly reduced the effect of the size of sequencing procedures.\ud \ud In the second experiment pupils with a low level of prior knowledge studied a text, following either a websequence or a linear sequence. Webteaching was superior to linear teaching on a number of dependent variables. It is concluded that webteaching is an effective sequencing procedure in those cases where substantial new learning is required

H2O Maser Observations of Candidate Post-AGB Stars and Discovery of Three High-velocity Water Sources

We present the results of 22 GHz H_2O maser observations of a sample of 85 post-Asymptotic Giant Branch (post-AGB) candidate stars, selected on the basis of their OH 1612 MHz maser and far-infrared properties. All sources were observed with the Tidbinbilla 70-m radio telescope and 21 detections were made. 86 GHz SiO Mopra observations of a subset of the sample are also presented. Of the 21 H_2O detections, 15 are from sources that are likely to be massive AGB stars and most of these show typical, regular H_2O maser profiles. In contrast, nearly all the detections of more evolved stars exhibited high-velocity H_2O maser emission. Of the five sources seen, v223 (W43A, IRAS 18450-0148) is a well known `water-fountain' source which belongs to a small group of post-AGB stars with highly collimated, high-velocity H_2O maser emission. A second source in our sample, v270 (IRAS 18596+0315), is also known to have high-velocity emission. We report the discovery of similar emission from a further three sources, d46 (IRAS 15445-5449), d62 (IRAS 15544-5332) and b292 (IRAS 18043-2116). The source d46 is an evolved post-AGB star with highly unusual maser properties. The H_2O maser emission from d62 is probably associated with a massive star. The source b292 is a young post-AGB star that is highly likely to be a water-fountain source, with masers detected over a velocity range of 210 km s^{-1}.Comment: 47 pages, 9 figures, 4 tables, accepted by Ap