Tree regeneration responds more to shade casting by the overstorey and competition in the understorey than to abundance per se

Manipulating the overstorey is the key tool for forest managers to steer natural regeneration. Opening up the canopy does not only create favourable light conditions for tree seedling growth, but also for (competitive) understorey species. Therefore, a thorough understanding of how changes in the abundance of the overstorey and understorey influence tree regeneration is needed to attain successful regeneration. To this end, we used the regional Flemish Forest Inventories, which contain vegetation plots that were surveyed at two times and include large variation in species composition and abundance of both overstorey and understorey layers. These plots were classified into poor and rich forest types, which differ in overstorey and understorey species composition and soil fertility. For each forest type, we first investigated the effect of overstorey abundance and shade-casting ability on the understorey herbaceous vegetation cover and its competitive nature. Then, we modelled how both these strata influence the presence-absence as well as the cover of tree regeneration, using the zero-inflated beta distribution. Our results show that the understorey cover and its competitiveness mainly increase when the abundance and shade-casting ability of the overstorey is reduced. The shade-casting ability of the overstorey and competitiveness of the understorey were more important in determining tree regeneration, especially probability of presence, than the abundance of these layers per se. This was consistent for both forest types, although directions and magnitudes of the effects differed. In predictions mimicking several thinning scenarios we found that in the poor forests, reducing overstorey abundance could lead to an increase in seedling cover, whereas in rich forests, the opposite is true and seedling cover will potentially be reduced. Finally, in a single-species analysis focusing on Quercus, we found a trade-off between sufficiently reducing overstorey abundance, while at the same retaining parent trees as potential seed sources. These findings can be used to guide forest management decisions in order to attain successful forest regeneration in temperate forests

Dark Ages woodland recovery and the expansion of beech : a study of land use changes and related woodland dynamics during the Roman to Medieval transition period in northern Belgium

The results from analyses of botanical remains (pollen, wood, charcoal, seeds) from several archaeological features excavated in Kluizen (northern Belgium) are presented. The region was largely uninhabited until the Iron Age and Roman period when a rural settlement was established, resulting in small-scale woodland clearance. The site was subsequently abandoned fromc.AD 270 till the High Middle Ages. The results of the archaeological and archaeobotanical analyses provide information on changes in land use and resulting dynamics of woodland cover and composition betweenc.600 BC and AD 1200, with a spatial and temporal resolution unrivalled in northern Belgium. Especially the long period of woodland regeneration following abandonment of the site around AD 270, covering the Late Roman and Early Medieval period, could be reconstructed in detail. Abandoned fields were first covered with pioneer woodland (Salix,CorylusandBetula), thenQuercus-dominated secondary forest and finally a late-successional forest withFagus sylvatica,Carpinus betulusandIlex aquifolium, an evolution that took over 300 years. The results also indicate that the observed increase ofFagusduring the Early Middle Ages, which was never an important element in the woodland vegetation in northern Belgium before, was related to climatic changes rather than anthropogenic factors

Identifying landscape drivers of genetic variation of a pond-breeding amphibian on multiple spatial scales

peer reviewe

Scale-dependent effects of terrestrial habitat on genetic variation in the great crested newt (Triturus cristatus)

peer reviewedContext Terrestrial landscapes surrounding aquatic habitat influence the persistence of amphibian spatially structured populations (SSPs) via their crucial role in providing estivation and overwintering sites, facilitating or hampering dispersal and colonisation, and consequently the maintenance or loss of genetic diversity. Objectives To highlight the landscape drivers of genetic variation, we investigated the relationship between the level of genetic variation measured within ponds of the great crested newt (Triturus cristatus), and the composition of the surrounding landscape at various spatial scales. Methods Based on the sampling of 40 ponds in 13 SSPs, the influence of landscape features on several estimators of genetic variation was investigated via linear mixed models, with effects within and between SSPs incorporated. Results The best models depended on the spatial scale, with more significant associations within radii of 50 and 100 m of core ponds, particularly for allelic richness. Responses within and between SSPs were mostly similar. The availability of aquatic habitat in the landscape had a positive effect, while woodland, arable land and pasture had different effects depending on scale and response variable. Total length of roads within a 250 m radius influenced effective population size negatively Conclusions Our results stress the need to investigate the influence of environmental predictors at multiple spatial scales for an adequate understanding of ongoing processes. Generally, the landscape affected genetic variation similarly within and between SSPs. This allowed us to provide general guidelines for the persistence of great crested newt populations, with an emphasis on the importance of the aquatic habitat

Responses of competitive understorey species to spatial environmental gradients inaccurately explain temporal changes

Understorey plant communities play a key role in the functioning of forest ecosystems. Under favourable environmental conditions, competitive understorey species may develop high abundances and influence important ecosystem processes such as tree regeneration. Thus, understanding and predicting the response of competitive understorey species as a function of changing environmental conditions is important for forest managers. In the absence of sufficient temporal data to quantify actual vegetation changes, space-for-time (SFT) substitution is often used, i.e. studies that use environmental gradients across space to infer vegetation responses to environmental change over time. Here we assess the validity of such SFT approaches and analysed 36 resurvey studies from ancient forests with low levels of recent disturbances across temperate Europe to assess how six competitive understorey plant species respond to gradients of overstorey cover, soil conditions, atmospheric N deposition and climatic conditions over space and time. The combination of historical and contemporary surveys allows (i) to test if observed contemporary patterns across space are consistent at the time of the historical survey, and, crucially, (ii) to assess whether changes in abundance over time given recorded environmental change match expectations from patterns recorded along environmental gradients in space. We found consistent spatial relationships at the two periods: local variation in soil variables and overstorey cover were the best predictors of individual species’ cover while interregional variation in coarse-scale variables, i.e. N deposition and climate, was less important. However, we found that our SFT approach could not accurately explain the large variation in abundance changes over time. We thus recommend to be cautious when using SFT substitution to infer species responses to temporal changes.</p

Forest microclimate dynamics drive plant responses to warming

Climate warming is causing a shift in biological communities in favor of warm-affinity species (i.e., thermophilization). Species responses often lag behind climate warming, but the reasons for such lags remain largely unknown. Here, we analyzed multidecadal understory microclimate dynamics in European forests and show that thermophilization and the climatic lag in forest plant communities are primarily controlled by microclimate. Increasing tree canopy cover reduces warming rates inside forests, but loss of canopy cover leads to increased local heat that exacerbates the disequilibrium between community responses and climate change. Reciprocal effects between plants and microclimates are key to understanding the response of forest biodiversity and functioning to climate and land-use changes

Varicella-zoster virus recapitulates its immune evasive behaviour in matured hiPSC-derived neurospheroids

peer reviewedVaricella-zoster virus (VZV) encephalitis and meningitis are potential central nervous system (CNS) complications following primary VZV infection or reactivation. With Type-I interferon (IFN) signalling being an important first line cellular defence mechanism against VZV infection by the peripheral tissues, we here investigated the triggering of innate immune responses in a human neurallike environment. For this, we established and characterised 5-month matured hiPSC-derived neurospheroids (NSPHs) containing neurons and astrocytes. Subsequently, NSPHs were infected with reporter strains of VZV (VZV eGFP-ORF23) or Sendai virus (SeV eGFP), with the latter serving as an immune-activating positive control. Live cell and immunocytochemical analyses demonstrated VZV eGFP-ORF23 infection throughout the NSPHs, while SeV eGFP infection was limited to the outer NSPH border. Next, NanoString digital transcriptomics revealed that SeV eGFP-infected NSPHs activated a clear Type-I IFN response, Frontiers in Immunology frontiersin.org 0

Replacements of small- by large-ranged species scale up to diversity loss in Europe’s temperate forest biome

The loss of biodiversity at the global scale has been difficult to reconcile with observations of no net loss at local scales. Vegetation surveys across European temperate forests show that this may be explained by the replacement of small-ranged species with large-ranged ones, driven by nitrogen deposition. Biodiversity time series reveal global losses and accelerated redistributions of species, but no net loss in local species richness. To better understand how these patterns are linked, we quantify how individual species trajectories scale up to diversity changes using data from 68 vegetation resurvey studies of seminatural forests in Europe. Herb-layer species with small geographic ranges are being replaced by more widely distributed species, and our results suggest that this is due less to species abundances than to species nitrogen niches. Nitrogen deposition accelerates the extinctions of small-ranged, nitrogen-efficient plants and colonization by broadly distributed, nitrogen-demanding plants (including non-natives). Despite no net change in species richness at the spatial scale of a study site, the losses of small-ranged species reduce biome-scale (gamma) diversity. These results provide one mechanism to explain the directional replacement of small-ranged species within sites and thus explain patterns of biodiversity change across spatial scales

Directional turnover towards larger-ranged plants over time and across habitats

Species turnover is ubiquitous. However, it remains unknown whether certain types of species are consistently gained or lost across different habitats. Here, we analysed the trajectories of 1827 plant species over time intervals of up to 78 years at 141 sites across mountain summits, forests, and lowland grasslands in Europe. We found, albeit with relatively small effect sizes, displacements of smaller- by larger-ranged species across habitats. Communities shifted in parallel towards more nutrient-demanding species, with species from nutrient-rich habitats having larger ranges. Because these species are typically strong competitors, declines of smaller-ranged species could reflect not only abiotic drivers of global change, but also biotic pressure from increased competition. The ubiquitous component of turnover based on species range size we found here may partially reconcile findings of no net loss in local diversity with global species loss, and link community-scale turnover to macroecological processes such as biotic homogenisation

Erratum to: Methods for evaluating medical tests and biomarkers

[This corrects the article DOI: 10.1186/s41512-016-0001-y.]