Bioaccumulation of cadmium, lead, and zinc in agriculture-based insect food chains

Globally, the metal concentration in soil is increasing due to different anthropogenic and geogenic factors. These metals are taken up by plants and further transferred in the food chain through different routes. The present study was designed to assess the transfer and bioaccumulation of the heavy metals, cadmium (Cd), lead (Pb), and zinc (Zn), in food chains from soil to berseem plants (Triofolium alexandrinum), to insect herbivores (the grasshopper Ailopus thalassinus and the aphid Sitobion avenae) and to an insect carnivore (the ladybird beetle Coccinella septempunctata). The soil of studied berseem fields were slightly alkaline, silty loam in texture and moderate in organic matter. In soil, the concentration of Zn and Pb were under permissible level while Cd was above the permissible level. The accumulation of metals in T. alexandrinum were found in the order Zn > Cd > Pb. Grasshoppers showed higher accumulation of Pb than of Cd and Zn. In the soil-berseem-aphid-beetle food chain, metal enrichment was recorded. However, aphids did not show bioaccumulation for Cd. Metals accumulation in beetles showed that translocation of Zn, Cd, and Pb was taking place in the third trophic level. Our study highlights the mobility of metals in insect food chains and showed that insect feeding style greatly influenced the bioaccumulation. However, different metals showed variable bioaccumulation rates depending on their toxicity and retention

Forensic Dentistry In Dire Need Of Recognition As A Separate Entity In Pakistan: A Cross-Sectional Study

Introduction: In this period of advancement, forensic dentistry plays crucial role in recognition of a person for ethical and the legal proceedings through the analysis of orofacial and dental characteristics with previous records. In this study, we aimed to assess the interest of dental professionals in participating in forensic dentistry courses and their willingness to introduce this subspecialty in the course curriculum of post graduate studies and to assess the knowledge, attitude and awareness about forensic odontology among dental professionals of Karachi, Pakistan. Methodology: This cross-sectional study was conducted through an online self-administered Knowledge, Attitude, and Practices (KAP) questionnaire with multiple close-end questions. In a convenient sample of 277 participants that includes academic faculty, undergraduates, postgraduates, house officers, dental care professional students (DCP) and dental technician/hygienist from Karachi, Pakistan. Data was measured using IBM SPSS version 26.0. Results: The survey showed that a significant number of study participants have knowledge of forensic dentistry as a branch 70.02% (p value 0.000) of the respondents showed interest to integrate forensic dentistry in the core curriculum. 62% of the Participants showed willingness to opt forensic dentistry as a career or profession. Those who were unwilling had various reasons. A significant number of people maintain dental records (P value = 0.026). Conclusion: There is need for up gradation and recognition of forensic dentistry into the curricula of dentistry to meet the international trends and pursue it as career