A membrane model for cytosolic calcium oscillations. A study using Xenopus oocytes

Cytosolic calcium oscillations occur in a wide variety of cells and are involved in different cellular functions. We describe these calcium oscillations by a mathematical model based on the putative electrophysiological properties of the endoplasmic reticulum (ER) membrane. The salient features of our membrane model are calcium-dependent calcium channels and calcium pumps in the ER membrane, constant entry of calcium into the cytosol, calcium dependent removal from the cytosol, and buffering by cytoplasmic calcium binding proteins. Numerical integration of the model allows us to study the fluctuations in the cytosolic calcium concentration, the ER membrane potential, and the concentration of free calcium binding sites on a calcium binding protein. The model demonstrates the physiological features necessary for calcium oscillations and suggests that the level of calcium flux into the cytosol controls the frequency and amplitude of oscillations. The model also suggests that the level of buffering affects the frequency and amplitude of the oscillations. The model is supported by experiments indirectly measuring cytosolic calcium by calcium-induced chloride currents in Xenopus oocytes as well as cytosolic calcium oscillations observed in other preparations

User-Defined Predicates in OPS5: A Needed Language Extension for Financial Expert Systems

OPS5 is widely used for expert system development in industry as well as for academic research. Its limited expressive power, however, can lead to cumbersome and inefficient code. Often a single domain rule must be encoded as a series of OPS5 rules requiring extensive performance overhead and resulting in an awkward representation of the knowledge. In the financial expert system ALEXSYS, which performs mortgage pool allocation, the lack of user-defined predicates proved to be a major obstacle, prohibiting real time performance. This work describes the addition of user-defined predicates in OPS5, supported by a patch to Carnegie-Mellon University's Common lisp OPS5 implementation. Also, the necessity of this extension is demonstrated in the context of the ALEXSYS mortgage pool allocation expert system, both in terms of increased efficiency and improved knowledge representation

A note on sampling and parameter estimation in linear stochastic systems

©1999 IEEE. Personal use of this material is permitted. However, permission to reprint/republish this material for advertising or promotional purposes or for creating new collective works for resale or redistribution to servers or lists, or to reuse any copyrighted component of this work in other works must be obtained from the IEEE.Numerical differentiation formulas that yield consistent least squares parameter estimates from sampled observations of linear, time invariant higher order systems have been introduced previously by Duncan et al. The formulas given by Duncan ct ai. have the same limiting system of equations as in the continuous time case. The formula presented in this note can be characterized as preserving asymptotically a partial integration rule. It leads to limiting equations for the parameter estimates that are different from the continuous case, but they again imply consistency. The numerical differentiation formulas given here can be used for an arbitrary linear system, which is not the ease in the previous paper by Duncan et al

Stochastic evolution equations driven by Liouville fractional Brownian motion

Let H be a Hilbert space and E a Banach space. We set up a theory of stochastic integration of L(H,E)-valued functions with respect to H-cylindrical Liouville fractional Brownian motions (fBm) with arbitrary Hurst parameter in the interval (0,1). For Hurst parameters in (0,1/2) we show that a function F:(0,T)\to L(H,E) is stochastically integrable with respect to an H-cylindrical Liouville fBm if and only if it is stochastically integrable with respect to an H-cylindrical fBm with the same Hurst parameter. As an application we show that second-order parabolic SPDEs on bounded domains in \mathbb{R}^d, driven by space-time noise which is white in space and Liouville fractional in time with Hurst parameter in (d/4,1) admit mild solution which are H\"older continuous both and space.Comment: To appear in Czech. Math.

Fundamental Science Investigations to Develop a 6-MV Laser Triggered Gas Switch for ZR: First Annual Report.

In October 2005, an intensive three-year Laser Triggered Gas Switch (LTGS) development program was initiated to investigate and solve observed performance and reliability issues with the LTGS for ZR. The approach taken has been one of mission-focused research: to revisit and reassess the design, to establish a fundamental understanding of LTGS operation and failure modes, and to test evolving operational hypotheses. This effort is aimed toward deploying an initial switch for ZR in 2007, on supporting rolling upgrades to ZR as the technology can be developed, and to prepare with scientific understanding for the even higher voltage switches anticipated needed for future high-yield accelerators. The ZR LTGS was identified as a potential area of concern quite early, but since initial assessments performed on a simplified Switch Test Bed (STB) at 5 MV showed 300-shot lifetimes on multiple switch builds, this component was judged acceptable. When the Z{sub 20} engineering module was brought online in October 2003 frequent flashovers of the plastic switch envelope were observed at the increased stresses required to compensate for the programmatically increased ZR load inductance. As of October 2006, there have been 1423 Z{sub 20} shots assessing a variety of LTGS designs. Numerous incremental and fundamental switch design modifications have been investigated. As we continue to investigate the LTGS, the basic science of plastic surface tracking, laser triggering, cascade breakdown, and optics degradation remain high-priority mission-focused research topics. Significant progress has been made and, while the switch does not yet achieve design requirements, we are on the path to develop successively better switches for rolling upgrade improvements to ZR. This report summarizes the work performed in FY 2006 by the large team. A high-level summary is followed by detailed individual topical reports