A Research Review on Tomato Bushy Stunt Virus Disease Complex

Tomato Bushy Stunt Virus (TBSV) was firstly reported on tomatoes by Smith in 1935 in England. The virus belongs to genus Tombusvirus and family Tombusviridae, is a soil-borne virus with isometric particle about 30 nm in diameter. Tomato Bushy Stunt Virus can cause chlorosis, necrosis, stunting, leaf yellowing, leaf mottling, leaf crinkling and fruit setting may be reduced or become zero. These symptoms were depending upon the host morphology. Transmission of this virus is naturally through infected seeds, propagative material and manually by the use of infective cutting tools. A numbers of varieties were affected. But it’s also observed that Lycopersicon pimpinellifolium not susceptible host plant. Gel Electropherotic analysis shows that virus distantly related serologically with several other viral species in the genus Tombusvirus. In phosphotungstic acid, the particles show an angular outline and unresolved surface structure but when mounted in uranyl acetate, they exhibit a rounded outline and somewhat knobby surface and edges. The viral genome is monopartite and TBSV-Ch has been completely sequenced and shown to contain 4,776 nucleotides. The protein shell is constructed by 180 subunits, each subunit contain Mr 41,000 and made icosahedral surface lattice. These subunits show a dimeric clustering on the surface of particles, which give rise to 90 morphological units. These were located on the two-fold axes of the lattice. Each coat protein subunit contains 387 amino acid residues and has four regions. Keywords: Tomato Bushy Stunt Virus, Genus Tombusvirus, Family Tombusviridae, Monopartite, Genome analysis of TBSV

Efficacy of exogenous application of 2, 4-Dichlorophenoxyacetic acid (2, 4-D) on growth and yield of mungbean (Vigna radiata L.)

The growth and yield promotion of mungbean in response to the use of 2, 4-Dichlorophenoxy acetic acid (2, 4-D) was investigated through a pot study which was arranged in the wire house of Institute of Soil and Environmental Sciences, University of Agriculture Faisalabad. The study was comprised of five treatments and three replications (control, 0.2 ppm 2, 4-D, 0.5 ppm 2, 4-D, 0.8 ppm 2, 4-D, and 1 ppm 2, 4-D). The suggested dose of Nitrogen, Phosphorus and Potassium (NPK) was applied at the amount of 20, 60 and 25 kg ha-1 added as Urea, DAP and SOP, respectively at sowing time. The data regarding growth (plant height, root and shoot length, root and shoot fresh and dry weight, and total biomass), yield (grain weight, number of grains plant-1) and NPK analysis in plants and soil was recorded and statistically analyzed. The response of exogenous application of 2, 4-D was significant at all levels in improving the performance of all the growth parameters and yield as compared to untreated control treatment. Maximum performance of all the parameters was recorded at 0.8 ppm application of 2, 4-D. As the concentration of 2, 4-D increased the development of plants also showed positive effect but up to 0.8 ppm application after that it started to decrease which showed that at higher concentrations 2, 4-D acts as growth retardant

Insecticide Screening For Effectiveness of Controlling Onion Thrips (Thrips Tabaci, Lindemann)

For the control of Thrips tabaci Lindeman Three insecticides product Movento240SC+Biopower276.6SL, Acephate75SP and Confidor200SL were applied against the onion (Allium cepa) thrips (Thrips tabaci Lindeman). The movento240SC+Biopower 276.6SP gave the highest results against the onion (Allium cepa) thrips (Thrips tabaci Lindeman) as compared to the other market standard insecticides of Acephate75SP and confidor200SL. The dose of Movento 240SP + Biopower 276.6SL 2.8 + 4.7 ml having more good results as compared to the other dose Movento 240SP +  Biopower 276.6 SL 2.4 + 4.7 ml. Keywords: onion thrips, insecticide use, insect contro