Correlation between CRP and IL-6 Serum Levels after Induction of Diabetes and Eight Weeks Resistance Training in Rats

Introduction: Systemic low-grade chronic inflammation is considered to be one of the significant characteristics of diabetes mellitus. This study aimed to investigate the correlation between C- reactive protein (CRP) and interleukin- 6 (IL-6) serum levels, after induction of diabetes and eight weeks of resistance training in rats. Methods: For the purpose of this experimental study, 24 streptozotocin induced diabetic rats were divided into three groups of 8 including (1) first week diabetic control, (2) last week diabetic control and (3) resistance training. Moreover, 8 healthy rats were chosen as the first week healthy control groups to assess the effects of diabetes induction on CRP and IL-6 serum levels. Rats of group 3 were subjected to an eight-week resistance training (3 days/week). Kolmogorov-Smirnov, independent-samples t-test, and Pearson correlation coefficient, were used for data analysis (p≤0.05). Results: Induction of diabetes had a significant effect on the increased serum levels of CRP (p = 0.01) and IL-6 (p = 0.01). However, after 8 weeks of resistance training, a significant decrease was observed in CRP (p = 0.001) and IL-6 (p = 0.01) serum levels of streptozotocin induced diabetic rats. Also, there was no significant correlation between CRP and IL-6 serum levels, after the induction of diabetes (p = 0.23) and eight weeks of resistance training (p=0.65). Conclusion: It appears that there is no correlation between CRP and IL-6 serum levels, after the induction of diabetes and eight weeks of resistance training in rats

The Effect of Eight Weeks of Curcumin Supplementation on the Expression of Some Regulatory Genes of Atrophic Processes in the Heart Tissue of Fatty Adult Fatty Rats

Background & Objective: Obesity causes heart muscle atrophy. The purpose of this study was to investigate the effect of curcumin supplement on expression of PGC1α, FoxO3a, MuRF1, MAFbx as regulators of cardiac muscle atrophy of rats. Material and Methods: 12 male Sprague Dawley rats were divided into two groups: obese (n = 6) and curcumin supplement (n = 6) after eight weeks of using high fat diet. The rats received curcumin for eight weeks and 150 mg / kg daily. The expression of genes was performed using Real Time-PCR technique. The expression of genes was calculated using the 2-∆∆CTmethod. One-way ANOVA was used to determine the significance of the variables among the study groups. Results: The administration of curcumin resulted in a significant reduction in the expression of genes of FoxO3a (P = 0.008), MAFbx (P = 0.02), MuRF1 (P = 0.001), and a significant increase in PGC1α gene expression (P = 0.004). Conclusion: Probably, administration of curcumin can be used to suppress heart muscle atrophy due to obesity by controlling the muscle atrophy pathway

Investigation of the Effect of High-Intensity Interval Training (HIIT) on the Expression of the Genes miR-126, miR-296, HGS and VEGF-A Protein Levels in Tumor Tissue in Female Mice

Background & Objective: The studies of the last two decades have shown that regular training is associated with the reduction of mortality among patients with breast cancer, and has an important role in inhibiting breast cancer progression as well as treatment of the disease. However, the micro-molecular mechanisms involved are not fully understood yet. However, the aim of the present study was to investigate the effect of a high-intensity interval training (HIIT) on the expression of miR-126, miR-296, HGS gene, and VEGF-A protein level in tumor tissue of the mice with breast cancer.   Materials & Methods: For this purpose, 12 BALB/c mice (6-8 weeks, weight 19 ± 1.05 g), after induction of cancer (MC4-L2 subcutaneous injection into the right side of the mice), were randomly divided into two groups with 6 in each group: HIIT and control. Each session of HIIT group program includes six intervals of three minutes and 20 seconds with an intensity of 85 to 90 percent of VO2max and 1-minute recovery with 30 to 35 percent VO2max intensity between each interval; and the animals were sacrificed 24 hours after the last training session and the expression of miR-126, miR-296, HBS gene and VEGF protein levels in tumor tissues were examined. Results: The statistical results showed that the implementation of HIIT significantly reduced the expression of miR-296 and subsequently increased the HGS gene expression and led to increased expression levels of miR-126 and decreased levels of VEGF-A protein in the training group compared to the control group (p<0.05). Conclusion: It seems that HIIT, by suppressing tumor angiogenesis signaling pathways, could be an effective intervention to inhibit the growth of breast tumors

Antioxidant Effects of Swimming Training and Curcumin in Withdrawal Period of Alcohol Overdose in Rats

Background & Objective: Alcohol overdose can induce increment in free radicals. The present study aimed to  investigate the effect of swimming training and Curcumin in withdrawal period of alcohol overdose on superoxide dismutase (SOD), glutathione peroxidase (GPX), total antioxidant capacity (TAC), malone dealdeid (MDA) and protein carbonyl (PC) of rats. Materials & Methods: 40 rats were selected and administered alcohol every 8 hours for 4 days. Then they were subjected to the withdrawal of alcohol for six days and in seventh day divided in 5 groups of 8 rats (1) control (2) Curcumin (3) swimming training (4) Curcumin and swimming training (5) sham. Groups 3 and 4 swam 5 sessions per week for 2 weeks and groups 2 and 4 used Curcumin 5 times per week for 2 weeks peritoneally. For statistical analysis of data two-way ANOVA was used (p≤0.05). Results: Swimming training had significant effect on increase of SOD (p=0.001), GPX (p=0.001) and TAC (p=0.009) and reduction of MDA (p=0.001) and PC (p=0.001) of rats. Curcumin had significant effect on increase of SOD (p=0.001) and GPX (p=0.001) and reduction of MDA (p=0.001) and PC (p=0.001) of rats but had no significant effect on TAC (p=0.34) also Curcumin and swimming training had interactive effects on increase of SOD (p=0.001) and GPX (p=0.001) and reduction of PC (p=0.009) of rats but had no interactional effect on TAC (p=0.48) and MDA (p=0.13). Conclusion: Probably, swimming training and curcumin can be applied simultaneously in withdrawal period of alcohol overdose to increase antioxidant factors