Colorism Among Black Youth in the United States: An Examination of Impacts on Education

Black students with darker complexions experience a disproportionate application of exclusionary practices within educational settings (Crutchfield et al., 2022). This review seeks to highlight colorism’s impact on the Black community in the education system through examining the historical context of colorism, the connection between colorism and racism, and how colorism is manifested and perpetuated in contemporary society in the United States. “Antiblackness” is an enduring trait of the United States that has rooted and fixed itself to its school structures (Coles & Powell, 2019). Schools are inherently political in that they adhere to and perpetuate the dominant ideologies of society (Picower & Mayorga, 2015), essentially functioning as a microcosm of society. Colorism functions as an extension of antiblackness in its perpetuation of prejudice and discriminatory behavior against darker skinned individuals and those with more African phenotypes. Black students are mandated by state-driven compulsory education laws to attend school but may receive differential treatment on the basis of skin tone, facial feature, and hair texture variations with the ethnic group. Exclusionary practices restrict students’ access to the school environment and may lead to a diminished academic identity, a sense of invisibility, feelings of invalidation, and limited access to future opportunities. In this review, the need for interventions addressing colorism in educational settings will be discussed. Recommendations for future directions of colorism research are also discussed

{The Noise Handling Properties of the Talbot Algorithm for Numerically Inverting the Laplace Transform}

This paper examines the noise handling properties of three of the most widely used algorithms for numerically inverting the Laplace Transform. After examining the genesis of the algorithms, the regularization properties are evaluated through a series of standard test functions in which noise is added to the inverse transform. Comparisons are then made with the exact data. Our main finding is that the Talbot inversion algorithm is very good at handling noisy data and is more accurate than the Fourier Series and Stehfest numerical inversion schemes as they are outlined in this paper. This offers a considerable advantage for it's use in inverting the Laplace Transform when seeking numerical solutions to time dependent differential equations.Peer reviewedFinal Published versio

Regional Motor Unit Firing Behaviors of Mono- and Bi-Articular Leg Extensor Muscles

Motor unit (MU) activation patterns provide vast insight into skeletal muscle contractions and may differ depending on architectural differences. Previous findings have suggested that MU activation patterns, specifically within the quadriceps group, are region-specific; this, along with the architectural differences between the quadriceps muscles, may further influence force production as reflected within the relationships between the firings. PURPOSE: To examine regional activation in proximal and distal regions of biarticular [rectus femoris (RF)] and monoarticular [vastus lateralis (VL)] knee extensors during submaximal isometric knee extensions. METHODS: On two separate randomized visits, eight lower-body resistance trained individuals, 6 males (n=6, age= 25.2 ±3.77) and 2 females (n=2, age= 21 ±1.4), performed submaximal isometric contractions at 30% and 70% of their maximal voluntary contractions (MVC) in a custom-built seat using an S-beam load-cell. Two separate 5-pin surface electromyography (EMG) sensors were used to record activation in the proximal and distal locations of either the VL or RF. Signals were recorded and decomposed into their constituent motor unit action potential (MUAP) trains, validated, and assessed for relative behavioral properties. For subsequent analysis of firing behaviors, the relationships (Slopes and intercepts) between motor unit action potential size (MUAPsize,) recruitment threshold (RT%), and mean firing rate (MFR) were calculated. Twelve separate two-way repeated measures analyses of variance (ANOVA) (location [proximal v distal] x muscle [VL v RF]) were used to compare slopes and intercepts of MFR vs. RT%, MUAPsize vs. RT%, and MFR vs. MUAPsize at both 30% and 70% MVC. RESULTS: There was a significant location x muscle interaction in the MFR v MUAPsize slopes during 30% MVC contraction (pCONCLUSION: The location by muscle interaction in the MFR v MUAPsize slopes during 30% MVC may indicate muscle fiber type distribution differences between sensor locations specifically, more type II fibers in the distal location of the VL

A cautionary tale of virus and disease

The recent identification of the gammaretrovirus XMRV and a second gammaretrovirus of a different subtype in chronic fatigue syndrome has aroused much interest, not least among sufferers. However, it remains highly controversial whether the detection of these viruses represents true infection or laboratory artifacts

Recombinant Complement Receptor 2 Radiolabeled with [Tc-99m(CO)(3)](+): A Potential New Radiopharmaceutical for Imaging Activated Complement

We describe the design and synthesis of a new Tc-99m labeled bioconjugate for imaging activated complement, based on Short Consensus Repeats 1 and 2 of Complement Receptor 2 (CR2), the binding domain for C3d. To avoid non specific modification of CR2 and the potential for modifying lysine residues critical to the CR2/C3d contact surface, we engineered a new protein, recombinant CR2 (rCR2), to include the C-terminal sequence VFPLECHHHHHH, a hexahistidine tag (for site-specific radiolabeling with [Tc-99m(CO)(3)(OH2)(3)](+)). The protein was characterized by N-terminal sequencing, SDS-PAGE and size exclusion chromatography. To test the function of the recombinant CR2, binding to C3d was confirmed by enzyme-linked immunosorbent assay (ELISA). The function was further confirmed by binding of rCR2 to C3d(+) red blood cells (RBC) which were generated by deposition of human or rat C3d and analyzed by fluorescence microscopy and flow cytometry. The affinity of rCR2 for C3d(+), in presence of 150 mM NaCl, was measured using surface plasma resonance giving rise to a K-D approximate to 500 nM. Radiolabeling of rCR2 or an inactive mutant of rCR2 (K41E CR2) or an unrelated protein of a similar size (C2A) with [Tc-99m(CO)(3)(OH2)(3)](+) at gave radiochemical yields >95%. Site-specifically radiolabeled rCR2 bound to C3d to C3d(+) RBC. Binding of radiolabeled rCR2 to C3d was inhibited by anti-C3d and the radiolabeled inactive mutant K41E CR2 and C2A did not bind to C3d(+) RBCs. We conclude that rCR2-Tc-99m has excellent radiolabeling, stability and C3d binding characteristics and warrants in vivo evaluation as an activated complement imaging agent

Anterior colporrhaphy does not induce bladder outlet obstruction

We aimed to evaluate if anterior colporrhaphy causes incomplete voiding due to bladder outlet obstruction. Women scheduled for anterior colporrhaphy were asked to undergo multichannel urodynamic investigation before surgery and the first postoperative day. Bladder outlet obstruction was assessed using the Blaivas-Groutz voiding nomogram. Maximum flow rate, detrusor pressure and residual volume were compared between pre- and postoperative measurements and between women with and without an abnormal post-void residual volume (PVR; volume exceeding 150 ml). Seventeen women participated. One woman who was unobstructed before surgery was obstructed after surgery. Overall, detrusor pressure and maximum flow rate before and after surgery did not differ. After surgery, six women had an abnormal PVR, one was unable to void, four were mildly obstructed and one moderately obstructed. Urodynamic investigation the first day after anterior colporrhaphy did not show that anterior colporrhaphy induces bladder outlet obstruction. The explanation for postoperative urinary retention can therefore also lie in non-anatomical causes such as postoperative pain and psychological factor

An Integrated TCGA Pan-Cancer Clinical Data Resource to Drive High-Quality Survival Outcome Analytics

For a decade, The Cancer Genome Atlas (TCGA) program collected clinicopathologic annotation data along with multi-platform molecular profiles of more than 11,000 human tumors across 33 different cancer types. TCGA clinical data contain key features representing the democratized nature of the data collection process. To ensure proper use of this large clinical dataset associated with genomic features, we developed a standardized dataset named the TCGA Pan-Cancer Clinical Data Resource (TCGA-CDR), which includes four major clinical outcome endpoints. In addition to detailing major challenges and statistical limitations encountered during the effort of integrating the acquired clinical data, we present a summary that includes endpoint usage recommendations for each cancer type. These TCGA-CDR findings appear to be consistent with cancer genomics studies independent of the TCGA effort and provide opportunities for investigating cancer biology using clinical correlates at an unprecedented scale. Analysis of clinicopathologic annotations for over 11,000 cancer patients in the TCGA program leads to the generation of TCGA Clinical Data Resource, which provides recommendations of clinical outcome endpoint usage for 33 cancer types

Plasma cytokines in women with chronic fatigue syndrome

© 2009 Fletcher et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution Licens

No Evidence for XMRV in German CFS and MS Patients with Fatigue Despite the Ability of the Virus to Infect Human Blood Cells In Vitro

BACKGROUND: Xenotropic murine leukemia virus-related virus (XMRV), a novel human retrovirus originally identified in prostate cancer tissues, has recently been associated with chronic fatigue syndrome (CFS), a disabling disease of unknown etiology affecting millions of people worldwide. However, several subsequent studies failed to detect the virus in patients suffering from these illnesses or in healthy subjects. Here we report the results of efforts to detect antibody responses and viral sequences in samples from a cohort of German CFS and relapsing remitting multiple sclerosis (MS) patients with fatigue symptoms. METHODOLOGY: Blood samples were taken from a cohort of 39 patients fulfilling the Fukuda/CDC criteria (CFS), from 112 patients with an established MS diagnosis and from 40 healthy donors. Fatigue severity in MS patients was assessed using the Fatigue Severity Scale (FSS). Validated Gag- and Env-ELISA assays were used to screen sera for XMRV antibodies. PHA-activated PBMC were cultured for seven days in the presence of IL-2 and DNA isolated from these cultures as well as from co-cultures of PBMC and highly permissive LNCaP cells was analyzed by nested PCR for the presence of the XMRV gag gene. In addition, PBMC cultures were exposed to 22Rv1-derived XMRV to assess infectivity and virus production. CONCLUSION: None of the screened sera from CFS and MS patients or healthy blood donors tested positive for XMRV specific antibodies and all PBMC (and PBMC plus LNCaP) cultures remained negative for XMRV sequences by nested PCR. These results argue against an association between XMRV infection and CFS and MS in Germany. However, we could confirm that PBMC cultures from healthy donors and from CFS patients can be experimentally infected by XMRV, resulting in the release of low levels of transmittable virus

Absence of evidence of Xenotropic Murine Leukemia Virus-related virus infection in persons with Chronic Fatigue Syndrome and healthy controls in the United States

<p>Abstract</p> <p>Background</p> <p>XMRV, a xenotropic murine leukemia virus (MuLV)-related virus, was recently identified by PCR testing in 67% of persons with chronic fatigue syndrome (CFS) and in 3.7% of healthy persons from the United States. To investigate the association of XMRV with CFS we tested blood specimens from 51 persons with CFS and 56 healthy persons from the US for evidence of XMRV infection by using serologic and molecular assays. Blinded PCR and serologic testing were performed at the US Centers for Disease Control and Prevention (CDC) and at two additional laboratories.</p> <p>Results</p> <p>Archived blood specimens were tested from persons with CFS defined by the 1994 international research case definition and matched healthy controls from Wichita, Kansas and metropolitan, urban, and rural Georgia populations. Serologic testing at CDC utilized a Western blot (WB) assay that showed excellent sensitivity to MuLV and XMRV polyclonal or monoclonal antibodies, and no reactivity on sera from 121 US blood donors or 26 HTLV-and HIV-infected sera. Plasma from 51 CFS cases and plasma from 53 controls were all WB negative. Additional blinded screening of the 51 cases and 53 controls at the Robert Koch Institute using an ELISA employing recombinant Gag and Env XMRV proteins identified weak seroreactivity in one CFS case and a healthy control, which was not confirmed by immunofluorescence. PCR testing at CDC employed a <it>gag </it>and a <it>pol </it>nested PCR assay with a detection threshold of 10 copies in 1 ug of human DNA. DNA specimens from 50 CFS patients and 56 controls and 41 US blood donors were all PCR-negative. Blinded testing by a second nested gag PCR assay at the Blood Systems Research Institute was also negative for DNA specimens from the 50 CFS cases and 56 controls.</p> <p>Conclusions</p> <p>We did not find any evidence of infection with XMRV in our U.S. study population of CFS patients or healthy controls by using multiple molecular and serologic assays. These data do not support an association of XMRV with CFS.</p