PERSPECTIVAS DE FRONTEIRA EM AUDIOLIVRO

UNIMPA JAGUARÃ

Transplante de células-tronco e suas possibilidades na endodontia

  Introduction: Maintaining the vitality of teeth is essential to ensure oral health and overall well-being. A vital tooth is one that retains its capacity for nutrition, sensitivity and self-repair, thanks to the presence of a healthy dental pulp inside. However, if this function is compromised by deep cavities, trauma, or infections, it is crucial to intervene with dental restorations to prevent irreversible loss. Preserving the health of the tooth is essential for maintaining the bone structure and stability of the dental arch, as well as preventing future complications such as serious infections, abscesses, and even tooth loss. In this context, stem cell transplantation emerges as a promising option to revitalize damaged pulp tissues, due to its unique ability to differentiate into several cell types, bringing a great potential for tissue repair and regeneration. Objective: Therefore, this study aims to analyze the possibilities and applications of stem cell transplantation in endodontics, in order to highlight its specific indications, procedures involved and the resulting benefits for patients suffering from a pulp condition. Methodology: This is an exploratory bibliographic research, of a descriptive nature, developed from articles contained in the PubMed/Medline, Virtual Health Library (VHL) and SciELO databases. The search for articles was carried out using the descriptors "Mesenchymal stem cell transplants", "Regenerative endodontics" and "Dental pulp diseases". Original articles, literature reviews, and case reports in Portuguese, English, and Spanish were included. Duplicate articles that were not the central theme of the research were discarded. Results: Stem cells can be classified as embryonic or adult, which reside in some tissues of the body, including the pulp. These cells can be isolated from permanent teeth (DPSCs) or deciduous teeth (SHEDs) and used in the restorative treatment of teeth with loss of pulp vitality. Conclusion: It is essential to conduct studies to improve isolation, cultivation and transplanting techniques, as well as to evaluate the safety and efficacy of these procedures in the long term. In addition, it is necessary to take into account regulatory and ethical issues.   Keywords: Mesenchymal stem cell transplants; Regenerative endodontics; Diseases of the dental pulp.Introdução: Manter a vitalidade dos dentes é fundamental para garantir a saúde bucal e o bem-estar geral. Um dente vital é aquele que conserva sua capacidade de nutrição, sensibilidade e autorreparação, graças à presença de uma polpa dentária saudável no seu interior. Contudo, se essa função for comprometida por cáries profundas, traumas ou infecções, é crucial intervir com restaurações dentárias para prevenir a perda irreversível. Preservar a saúde do dente é essencial para manter a estrutura óssea e a estabilidade da arcada dentária, além de prevenir complicações futuras como infecções graves, abscessos e até mesmo a perda dos dentes. Nesse contexto, o transplante de células-tronco desponta como uma opção promissora para revitalizar tecidos pulpares danificados, devido à sua capacidade única de se diferenciar em diversos tipos celulares, trazendo um grande potencial de reparação e regeneração tecidual. Objetivo: Diante disso, este estudo objetiva analisar as possibilidades e aplicações do transplante de células-tronco na endodontia, com o intuito de destacar suas indicações específicas, procedimentos envolvidos e os benefícios resultantes para pacientes que sofrem com alguma condição pulpar. Metodologia: Trata-se de uma pesquisa bibliográfica exploratória, de caráter descritivo, desenvolvida a partir de artigos contidos nas bases de dados PubMed/Medline, Biblioteca Virtual em Saúde (BVS) e SciELO. A busca por artigos foi realizada fazendo uso dos descritores “Transplantes de células-tronco mesenquimais”, “Endodontia regenerativa” e “Doenças da polpa dentária”. Foram incluídos artigos originais, revisões de literatura e relatos de caso, em português, inglês e espanhol. Foram descartados artigos duplicados e que fugiam do tema central da pesquisa. Resultado: As células-tronco podem ser classificadas em embrionárias ou adultas, que residem em alguns tecidos do organismo, inclusive no pulpar. Essas células podem ser isoladas de dentes permanentes (DPSCs) ou decíduos (SHEDs) e usadas no tratamento restaurador de dentes com perda de vitalidade pulpar. Conclusão: É fundamental conduzir estudos para aprimorar as técnicas de isolamento, cultivo e transplante, bem como avaliar a segurança e eficácia desses procedimentos a longo prazo. Além disso, é necessário levar em conta questões regulatórias e éticas.   Palavras-chave: Transplantes de células-tronco mesenquimais; Endodontia regenerativa; Doenças da polpa dentária

Conflito trabalho-família em profissionais do contexto hospitalar: análise de preditores

The present study investigates the predictive aspects of the work-family conflict in profes-sionals in the hospital context. Such contexts are commonly investigated in terms of health domains and organizational factors, with effects on aspects of work-family interaction still little explored. Research on the work-family relationship is important both for organiza-tions, for families, professionals, and society. Therefore, a survey study was conducted, with 470 workers, with a mean age of 34.50 years and an average years working in the profession of 8.0 years. Participants answered a survey composed of questions and psychological scales. The results pointed out that aspects of family interference at work were predicted by age, career time, intention to leave the organization and organizational incivility, and work to interfere in the family also involved aspects of workload, work satisfaction, organizational commitment and civility organizational structure. It is discussed suggestions for manage-ment and research on the subject in organizationsLa présente étude a examiné les aspects prédictifs du conflit travail-famille chez les profes-sionnels en milieu hospitalier. Tels contextes font généralement l’objet d’une recherche en termes de domaines de la santé et de facteurs organisationnels, et les effets sur les aspects de l’interaction travail-famille sont encore peu explorés. La recherche sur la relation tra-vail-famille est importante pour les organisations, les familles, les professionnels et la société. Par conséquent, une étude d’enquête sur les données a été menée auprès de 470 travailleurs, dont l’âge moyen était de 34.50 et de 8.0 ans d’expérience professionnelle en moyenne. Les participants ont répondu à un questionnaire composé de questions et d’échelles psycho-logiques. Les aspects de l’ingérence familiale au travail étaient prédits en fonction de l’âge, de la durée de la carrière, de l’intention de quitter l’organisation et des incivilités organisa-tionnelles. Des suggestions de gestion et de recherche sur le sujet dans les organisations ont été discutéesO presente estudo pesquisou os aspectos preditivos do conflito trabalho-família em pro-fissionais de contextos hospitalares. Tais contextos são comumente pesquisados em termos de domínios de saúde e fatores organizacionais, sendo o conflito da interação trabalho--família ainda pouco explorado. Pesquisas sobre a relação trabalho-família são importantes tanto para as organizações, quanto para as famílias, os profissionais e a sociedade. Portanto, conduziu-se um estudo de levantamento de dados, com 470 trabalhadores, com média de idade de 34.5 anos e tempo de profissão médio de 8 anos. Os participantes responderam um questionário composto de preguntas e escalas psicológicas. Os resultados apontaram que aspectos da interferência da família no trabalho foram preditos por idade, tempo de carreira, intenção de deixar a organização e incivilidade organizacional, e a interferência do trabalho na vida familiar envolveu adicionalmente aspectos da carga horária de trabalho, a satisfação laboral, o compromisso organizacional e a civilidade organizacional. Discutem-se sugestões para a gestão e pesquisa sobre o tema em organizaçõesEl presente estudio investiga los aspectos predictivos del conflicto trabajo-familia en pro-fesionales de hospitales. Tales contextos son comúnmente investigados en los dominios de salud y factores organizacionales, siendo el conflicto trabajo-familia poco explorado aún. Las investigaciones sobre la relación trabajo-familia son importantes para las organizaciones, las familias, los profesionales y la sociedad. Se realizó un estudio de levantamiento de datos, con 470 trabajadores con una edad media de 34.5 años; y una media de 8 años de tiempo de profesión. Los participantes respondieron un cuestionario compuesto de preguntas y escalas psicológicas. Aspectos de la interferencia de la familia en el trabajo fueron predichos por la edad, el tiempo de carrera, la intención de dejar la organización y la incivilidad organiza-cional; la interferencia del trabajo en la vida familiar involucró adicionalmente aspectos de la carga horaria del trabajo, la satisfacción laboral, el compromiso organizacional y la civilidad organizacional. Se discutieron sugerencias para la gestión y la investigación sobre el tema en las organizaciones

Brain Research

Acesso restrito: Texto completo. p. 188-196Parkinson's disease (PD) is one of the most important neurodegenerativeworldwide disorders. It is characterized by a selective and progressive degeneration of dopaminergic neurons, causing a series of symptoms which might ultimately induce programmed cell death. The potential cytoprotective effects of one of the commercial extracts of Anemopaegma mirandum (Catuaba), a Brazilian tree, on Rotenone-induced apoptosis in human neuroblastomas SH-SY5Y cells was demonstrated. The cell viability, analysis of cellular morphology, nuclei morphology and ultra structural research were done by MTT-tetrazole (3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyltetrazolium bromide) assay, phase contrast microscopy, stained with Hoechst 33258 and electron microscopy transmission, respectively. Three different concentrations of Catuaba extract were used (0.312, 0.625 and 1.250 mg/mL). These extracts promoted an increase of 22.3± 3.6%, 22.0±2.1% and 15.8±0.7% on the cell viability. Notable changes in the cellularmorphology, condensation of the cell body, nuclear fragmentation and condensation into discrete dense chromatin clumps were observed when the cells were treated with 300 nM Rotenone for 48 h. These effectswere partially alteredwhen the extract of A. mirandum was added to the Rotenone treatment. Ultra structural analysis by electron microscopy demonstrated that citoplasmatic membranes and mitochondria membrane were also clearly preserved in the group treatedwith the extract. Therefore, in this study, our findings indicated that extracts of A. mirandum have cytoprotective effects on Rotenone-induced apoptosis in humanneuroblastomas SH-SY5Y cells

Association Between Hepatitis C and Hepatocellular Carcinoma

Hepatocellular carcinoma (HCC) is the fifth most common cancer, the third most common cause for cancer death in the world, a major cause of death in patients with chronic hepatitis C virus infection, and responsible for approximately one million deaths each year. Overwhelming lines of epidemiological evidence have indicated that persistent infection with hepatitis C virus (HCV) is a major risk for the development of HCC. The incidence of HCC is expected to increase in the next two decades, largely due to hepatitis C infection and secondary cirrhosis, and detection of HCC at an early stage is critical for a favorable clinical outcome. Potential preventive strategies in the development of HCC are being recognized. The natural history of HCC is highly variable and the clinical management choices for HCC can be complex, hence patient assessment and treatment planning have to take the severity of the nonmalignant liver disease into account. This review summarizes the inter-relationship between HCV and liver carcinogenesis

Neurochemical Research

Acesso restrito: Texto completo. p. 215-220.Parkinson0s disease (PD) is one of the most important neurodegenerative worldwide disorders. The potential cytoprotective effects of aqueous extract of Valeriana officinalis on rotenone-induced apoptosis in human neuroblastoma SH-SY5Y cells were demonstrated. The cytotoxicity, cell viability and analysis of cellular morphology were performed by MTT-tetrazole (3-(4,5- dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay and phase contrast microscopy, respectively. Significant changes in the cellular morphology, and condensation of the cell body could be observed when cells were treated with 300 nM rotenone for 48 h. Three different concentrations of Valeriana officinalis extract were used (0.049, 0.098 and 0.195 mg/mL). These extracts brought about an increase of 7.0 ± 1.3%, 14.5 ± 1.3% and 14.5 ± 3.2% in cell viability. Our results indicated that neuroprotector action of the Valeriana officinalis extract provides support for later studies as they help understanding this drug for the development of cytoprotective various therapies in PD