Anti-inflammatory and Antinociceptive Activity of Ouabain in Mice

Ouabain, an inhibitor of the Na+/K+-ATPase pump, was identified as an endogenous substance of human plasma. Ouabain has been studied for its ability to interfere with various regulatory mechanisms. Despite the studies portraying the ability of ouabain to modulate the immune response, little is known about the effect of this substance on the inflammatory process. The aim of this work was to study the effects triggered by ouabain on inflammation and nociceptive models. Ouabain produced a reduction in the mouse paw edema induced by carrageenan, compound 48/80 and zymosan. This anti-inflammatory potential might be related to the inhibition of prostaglandin E2, bradykinin, and mast-cell degranulation but not to histamine. Ouabain also modulated the inflammation induced by concanavalin A by inhibiting cell migration. Besides that, ouabain presented antinociceptive activity. Taken these data together, this work demonstrated, for the first time, that ouabain presented in vivo analgesic and anti-inflammatory effects

Effects of the single supplementation and multiple doses of Passiflora incarnata L. on human anxiety: A Clinical Trial, Double-blind, Placebo-controlled, Randomized

Object: This study aimed to investigate the effects of Passiflora incarnata L. on anxiety in humans. Method: The individuals were randomly assigned to one of the following groups: Placebo or experimental, n =30, single dose, as well as Placebo or Experimental, n =15, multiple dose.  The experimental human anxiety was induced by simulated public speaking test in the following phases: Basal (B), stressful (A), speech 1 (S1), speech 2 (S2) and Final (F). We evaluated the Systolic Blood Pressure (SBP), and Diastolic (DBP), Heart Rate (HR), Electrical Conductance of Skin and extremities temperature (ET) and filled The state-trait anxiety inventory (STAI S e T). Results: During the single dose, the HR was reduced at the end of the speech (86±2,0 to 74±3,0 bpm) the experimental group compared to the placebo group (p<0,05); the STAI-S did not change. In the delineation of multiple doses, SBP was reduced, in mmHg, in the experimental group compared to the placebo group during all phases. Experimental: 106±1,0 (B), 111±1,0 (A), 121±2,0 (S1), 115±3 (S2), 104±2,0 (F) e Placebo: 121±3,0; 127±3,0; 130±3,0; 130±4,5; 117±3,0 (p<0,05). Conclusions: It is suggested that supplementation using Passiflora incarnata L. Capsules (500 mg) decreased cardiovascular signals both in single and in multiple doses, associated with the stress of public speaking

Efecto analgésico del extracto hidroalcoholico de las hojas de Cissampelos sympodialis Eichl

Existen varios estudios que evidencian que la Cissampelos sympodialis posee atividad anti-inflamatoria, antialérgica, antidepresiva y de baja toxicidad. Este estudio evaluó los efectos de la administración intraperitoneal, del extracto hidroalcohólico de hojas de cissampelos sympodialis (EHACS), en las dosis de 50, 100 y 200 mg/Kg, en modelos de analgesia en camumdongos. En el modelo de las contracciones inducidas por el ácido acético (0,85%) fue encontrada una reducción en el número de contracciones proporcional a las dosis usadas (p<0,05). En el test de la reacción al formaldeído las dosis utilizadas no presentaron efecto significante en la primera fase del test (0-5 minutos). Sin embargo, la dosis de 200mg/Kg presentó una significante inhibición en la segunda fase (15 – 30 minutos) del test (p<0,05). Los resultados mostraron que el EHACS presentó una actividad antinociceptiva periférica probablemente relacionada con la alegada actividad anti-inflamatoria

Synthesis of New Imidazolidin-2,4-dione and 2-Thioxoimidazolidin-4-ones via C-Phenylglycine Derivatives

Hydantoins and their derivatives constitute a group of pharmaceutical compounds with anticonvulsant and antiarrhythmic properties, and are also used against diabetes. N-3 and C-5 substituted imidazolidines are examples of such products. As such, we have developed a synthesis of 2,4-dione and 2-thioxo-4-one imidazolidinic derivatives by reaction of amino acids with C-phenylglycine, phenyl isocyanate and phenyl isothiocyanate. Four amino-derivatives IG(1-4) and eight imidazolidinic derivatives, IM(1-8), were obtained in yields of 70–74%. The mass, infrared, 1H and 13C-NMR spectra of representative products are discussed

Molecules. The coauthor Raline Mendonça dos Anjos was omitted from the author list, which should

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Anxiolytic-like activity and GC–MS analysis of (R)-(+)-limonene fragrance, a natural compound found in foods and plants

AbstractThe traditional use of essential oils in aromatherapy has offered numerous health benefits. However, few scientific studies have been conducted with these oils to confirm their therapeutic efficacy. (+)-Limonene is a chemical constituent of various bioactive essential oils. The present study reports on the anxiolytic-like effects of (+)-limonene in an elevated maze model of anxiety in mice. At concentrations of 0.5% and 1.0%, (+)-limonene, administered to mice by inhalation, significantly modified all the parameters evaluated in the elevated plus maze test. The pharmacological effect of inhaled (+)-limonene (1%) was not blocked by flumazenil. Analysis of (+)-limonene using gas chromatography–mass spectrometry (GC–MS) showed its volatility to be high. These data suggest possible connections between the volatility of (+)-limonene and its anxiolytic-like effect on the parameters evaluated in the elevated plus maze test. The data indicate that (+)-limonene could be used in aromatherapy as an antianxiety agent