Effects of platelet-rich fibrin produced by three centrifugation protocols on bone neoformation in defects created in rat calvaria.

This study evaluated the potential of Leukocyte-platelet-rich fibrin (L-PRF; fixed angle centrifugation protocol), Advanced-platelet-rich fibrin (A-PRF; low-speed fixed angle centrifugation protocol), and Horizontal-platelet-rich fibrin (H-PRF; horizontal centrifugation protocol) in bone neoformation in critical size defects (CSDs) in rat calvaria. Thirty-two rats were divided into groups: Control (C), L-PRF, A-PRF, and H-PRF. 5 mm diameter CSDs were created in the animals' calvaria. Defects from group Control (C) were filled with blood clots, while defects from groups L-PRF, A-PRF, and H-PRF were filled with respective platelet-rich fibrin (PRF) membranes. L-PRF, A-PRF, and H-PRF were prepared from animal blood collection and specific centrifugation protocols. At 14 and 30 days, calcein (CA) and alizarin (AL) injections were performed, respectively. Animals were euthanized at 35 days. Microtomographic, laser confocal microscopy, and histomorphometric analyzes were performed. Data were statistically analyzed (ANOVA, Tukey, p < .05). L-PRF, A-PRF, and H-PRF groups showed higher values of bone volume (BV), newly formed bone area (NFBA), and precipitation of CA and AL than the C group (p < .05). The H-PRF group showed higher values of BV, number of trabeculae (Tb. N), NFBA, and higher precipitation of AL than the A-PRF and L-PRF groups (p < .05). Therefore, it can be concluded that: i) L-PRF, A-PRF, and H-PRF potentiate bone neoformation in CSDs in rat calvaria; ii) H-PRF demonstrated more biological potential for bone healing

Comparison of the effects of platelet aggregates produced by high and low-speed centrifugation protocols on the healing of critical-size defects in rat calvaria: a microtomographic and histomorphometric study

O presente estudo avaliou a cicatrização de defeitos de tamanho crítico (DTC) criados em calvária de ratos e tratados com agregados plaquetários produzidos por protocolos de alta velocidade (Fibrina Rica em Plaquetas e Leucócitos - L-PRF) e baixa velocidade (Fibrina Rica em Plaquetas e Leucócitos Avançada - A-PRF) de centrifugação. Vinte e quatro ratos foram divididos em 3 grupos: Controle (C), L-PRF e A-PRF. DTC de 5 mm de diâmetro foram criados na calvária dos animais. Os defeitos do Grupo C foram preenchidos com coágulo sanguíneo somente. Os defeitos dos grupos L-PRF e A-PRF foram preenchidos com 0,2 ml de L-PRF e A-PRF, respectivamente. Todos os animais foram submetidos à eutanásia aos 35 dias pós-operatórios. Análises histomorfométricas e microtomográficas foram realizadas. Os dados foram estatisticamente analisados (Anova, Tukey, p<0,05). Os grupos L-PRF e A-PRF apresentaram valores de Volume Ósseo e de Área de Osso Neoformado significativamente maiores que àqueles do grupo C, bem como menores valores de Porosidade Óssea (p<0,05). Não foram observadas diferenças significativas entre os grupos A-PRF e L-PRF para esses parâmetros analisados. Pode-se concluir que i) L-PRF e A-PRF potencializaram a cicatrização de DTC em calvária de ratos; ii) protocolos de alta e baixa velocidade de centrifugação não produziram matrizes de agregados plaquetários com impactos biológicos diferentes na quantidade de neoformação óssea.The current study evaluated the healing of critical-size defects (CSD) created in rat calvaria treated with platelet aggregates produced by high-speed (Leukocyte- and Platelet-Rich Fibrin - L-PRF) and low-speed (Advanced Platelet-Rich Fibrin - A-PRF) protocols of centrifugation. Twenty-four rats were divided into 3 groups: Control (C), LPRF, and A-PRF. Five mm diameter CSD were created on the animals\' calvaria. The defects of the L-PRF and A-PRF groups were filled with 0.01 ml of L-PRF and A-PRF, respectively. The C group defects were filled with a blood clot only. All animals were euthanized on the 35th postoperative day. Histomorphometric and microtomographic analysis were then performed. The L-PRF and A-PRF groups had significantly higher bone volume and neoformed bone area than those of the C group, as well as lower bone porosity values (p<0.05). No significant differences were observed between A-PRF and L-PRF groups for the analyzed parameters. It can be concluded that i) L-PRF and A-PRF potentiated the healing of CSD in rat calvaria; ii) high and low-speed centrifugation protocols did not produce PRF matrices with different biological impacts on the amount of bone neoformation

Comparison of the effects of platelet concentrates produced by high and low-speed centrifugation protocols on the healing of critical-size defects in rat calvaria: a microtomographic and histomorphometric study.

The current study evaluated the healing of critical-size defects (CSD) created in rat calvaria treated with platelet concentrates produced by high-speed (Leukocyte- and Platelet-Rich Fibrin - L-PRF) and low-speed (Advanced Platelet-Rich Fibrin - A-PRF) protocols of centrifugation. Twenty-four rats were distributed into three groups: Control, L-PRF, and A-PRF. Five mm diameter CSD were created on the animals' calvaria. The defects of the L-PRF and A-PRF groups were filled with 0.01 ml of L-PRF and A-PRF, respectively. The control group defects were filled with a blood clot only. All animals were euthanized on the 35th postoperative day. Histomorphometric and microtomographic analyses were then performed. The L-PRF and A-PRF groups had significantly higher bone volume and neoformed bone area than those of the control group and lowered bone porosity values (p < .05). No significant differences were observed between A-PRF and L-PRF groups for the analyzed parameters. Therefore, it can be concluded that i) L-PRF and A-PRF potentiated the healing of CSD in rat calvaria; ii) high and low-speed centrifugation protocols did not produce PRF matrices with different biological impacts on the amount of bone neoformation