13 research outputs found

    Characterization of a murine model of septic cachexia

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    AsiFood and its output and prospects: An Erasmus+ project on capacity building in food safety and quality for South-East Asia

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    The Asifood project is a capacity building project in the field of higher education involving collaboration among thirteen partners from Cambodia, Thailand, Vietnam, Austria, Belgium, Italy and France. This project aimed to support the universities in Vietnam, Thailand and Cambodia in building their capacities and their link with professionals in food safety and food quality, in the context of ASEAN integration. Further, training for trainers around a key theme, ‘food safety and quality’ for partner countries was set up involving students and teachers, professional stakeholders, political decision-makers and association leaders. During the first year of the project, study and diagnostic phase were carried out to properly assess the training as per each university needs. In the second year, the training paths around three axes: courses, quality and laboratory analysis were conducted. Finally, a test phase was carried out with the partners by inserting the modules created in the bachelor's and master's degree courses offered by the universities as well as short term trainings on innovations in food safety and quali

    Mice Survival and Plasmatic Cytokine Secretion in a “Two Hit” Model of Sepsis Depend on Intratracheal <i>Pseudomonas Aeruginosa</i> Bacterial Load

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    <div><p>Sepsis is characterized by pro- and anti-inflammatory responses following infection. While inflammation is responsible for widespread organ damage, anti-inflammatory mediators lead to immunoparalysis increasing susceptibility to secondary infections (nosocomial pneumonia). We aimed to investigate the impact of bacterial load on survival and cytokine release in a two-hit murine (C57BL/6J) model of CLP followed by <i>P</i>. <i>aeruginosa</i> pneumonia. Plasmatic TNFα, IL-6, IL-10, sTNFr I and II were quantified until 13 days. At D5, splenocytes were processed for immunological assays or mice were intratracheally instilled with <i>Pseudomonas aeruginosa</i> (5.10<sup>6</sup>, 2.10<sup>7</sup> and 10<sup>8</sup> CFU) to evaluate survival and cytokines production. TNFα, sTNFrs, IL-6 and IL-10 increased 2h post CLP. TNFα and sTNFrs declined respectively one and two days later. In CLP mice, IL-6 and IL-10 remained high for the whole experiment, as compared to Sham. At D5, for CLP mice, whereas total T cells population (CD3+) decreased, Treg fraction (CD4+/CD25+) increased. In parallel, T cells proliferation and LPS-stimulated splenocytes ability to release TNFα decreased. At D13, survival was 100% after 5.10<sup>6</sup> CFU, 50% for CLP mice after 2.10<sup>7</sup> CFU and 0% for CLP and Sham after 10<sup>8</sup> CFU. After instillation, IL-10 and IL-6 increased and appeared to be dose and time dependent. <i>Pseudomonas</i> was detected in all CLP and Sham’s lungs; in spleen and liver only in CLP at 2.10<sup>7</sup> CFU, and in CLP and Sham at 10<sup>8</sup> CFU. We demonstrated that post-CLP immunosuppression followed by <i>Pseudomonas aeruginosa</i> lung instillation increases mortality reactivates cytokines secretion and is associated with systemic dissemination in septic mice depending on bacterial load.</p></div
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