Chloroplast genomes of Vitis sp. samples from Nechaevsk population of the Krasnodar region

The presented study demonstrates the results of sequencing and analysis of wild grape plastomes assemblies located in the Nechaevsk population. Previously, the habitats of the wild forest grape V. vinifera subsp. silvestris were explored as an outcome of expeditions. As a result of repeated expeditions, samples were taken and used to isolate chloroplast DNA. Assembly and annotation of plastomes showed the presence of DNA encoding sequences. At the same time, the structure of the obtained plastomes varied. These expressed in the presence of DNA polymorphisms. Comparison of the aligned sequences made it possible to reveal the presence of the species V. vinifera subsp. sylvestris in the population, as well as hybrid specimens. This is also confirmed by the result of determining the taxonomy after depositing the archives of readings in the NCBI database

Study of the VvMybA1 gene allele state of some colored and uncolored grapevine varieties

As a result of the work, genetic markers were created that were used to identify alleles of the VvMybA1 genes, which control the anthocyanin color of berries in grape varieties. Using these markers, alleles were identified in grape varieties belonging to different ecologicalgeographical groups. In the process of work, alleles were sequenced and compared with the NCBI database in order to identify their state and their belonging to a specific type of structure. In addition, these sequences were compared with each other to reveal the structural features of varieties with and without color of berries. Single nucleotide polymorphisms, structural features, and deletions in the structure of alleles were revealed