The invasive kudzu bug, Megacopta cribraria (Hemiptera: Plataspidae) as an agricultural and nuisance pest

The invasive “kudzu bug,” Megacopta cribraria (Hemiptera: Plataspidae) is a native of Asia that was found in northeastern Georgia in fall 2009. This insect has since spread rapidly throughout the southeastern U.S., and is abundant in much of South Carolina. Megacopta cribraria feed on the vascular fluid of several legumes, primarily kudzu, Puereria montana var. lobata, and soybeans, Glycine max. These insects enter soybean fields in often alarming numbers, leaving conspicuous lesions on soybean stems and potentially reducing yields. In addition, M. cribraria congregate on homes and other structures in late fall as they seek overwintering sites, potentially becoming a nuisance pest where populations are large. We examined M. cribraria as a yield reducing pest of soybeans and as a nuisance pest of buildings. Large populations of M. cribraria in caged soybean plots resulted in yield losses of up to 59.6%, and yield reductions were similar in both 2011 and 2012. Several professional-use insecticides were effective in controlling M. cribraria on a variety of building material surfaces, and in some insecticide × surface combinations activity was maintained 30 days post-application. These results contribute to our still developing understanding of this insect as an agricultural and nuisance pest

Nest Emigration Behavior of the Asian Needle Ant, Brachyponera (=Pachycondyla) chinensis Emery (Hymenoptera: Formicidae)

Ant colonies change nest location in response to physical disturbance, climate fluctuation, and resource availability. During the emigration process, worker recruitment is vital to ensuring that individual colony members are moved to the new nest site. Recruitment methods used during emigration differ between ant species. In a laboratory study, we investigated the recruitment behaviors of the invasive Asian needle ant, Brachyponera (=Pachycondyla) chinensis (Emery), during nest emigration. Subsets of P. chinensis worker ants were subjected to physical nest disturbance, and the recruitment methods and associated behaviors were recorded. Before recruitment to the new nest location began, B. chinensis ants organized into three distinctive groups: queen-tending, brood-tending, and scouting. Once the new nest site was identified, scout ants began physically transporting nestmates into the new harborage. Transport rates increased with time in the first 30 minutes and did not change during the 30to 55 minute interval when brood was transported. However, adult transport rate increased again after brood transport was completed and decreased after 90 minutes. These studies are the first to identify the recruitment methods, division of labor, and social organization behavior of B. chinensis during nest emigration

Results and Figures - Nest Emigration in Brachyponera chinensis

Ant colonies change nest location in response to physical disturbance, climate fluctuation, and resource availability. During the emigration process, worker recruitment is vital to ensuring that individual colony members are moved to the new nest site. Recruitment methods used during emigration differ between ant species. In a laboratory study, we investigated the recruitment behaviors of the invasive Asian needle ant, Brachyponera (=Pachycondyla) chinensis (Emery), during nest emigration. Subsets of P. chinensis worker ants were subjected to physical nest disturbance, and the recruitment methods and associated behaviors were recorded. Before recruitment to the new nest location began, B. chinensis ants organized into three distinctive groups: queen-tending, brood-tending, and scouting. Once the new nest site was identified, scout ants began physically transporting nestmates into the new harborage. Transport rates increased with time in the first 30 minutes and did not change during the 30to 55 minute interval when brood was transported. However, adult transport rate increased again after brood transport was completed and decreased after 90 minutes. These studies are the first to identify the recruitment methods, division of labor, and social organization behavior of B. chinensis during nest emigration

Climbing ability of teneral and sclerotized adult bed bugs and assessment of adhesive properties of the exoskeletal fluid using atomic force microscopy

We observed that teneral adults (\u3c1 h post-molt) of Cimex lectularius L. appeared more adept at climbing a smooth surface compared to sclerotized adults. Differences in climbing ability on a smooth surface based on sclerotization status were quantified by measuring the height to which bed bugs climbed when confined within a glass vial. The average maximum height climbed by teneral (T) bed bugs (n = 30, height climbed = 4.69 cm) differed significantly (P\u3c 0.01) from recently sclerotized (RS) bed bugs (n = 30, height climbed = 1.73 cm at ~48 h post molt), sclerotized group 1 (S1) bed bugs (n = 30, S1 = 2.42 cm at \u3e72 h), and sclerotized group 2 (S2) bed bugs (n = 30, height climbed = 2.64 cm at \u3e72 h post molt). When heights from all climbing events were summed, teneral bed bugs (650.8 cm climbed) differed significantly (P\u3c 0.01) from recently sclerotized (82 cm climbed) and sclerotized (group 1 = 104.6 cm climbed, group 2 = 107.8 cm climbed) bed bugs. These findings suggested that the external surface of teneral bed bug exoskeletons possess an adhesive property. Using atomic force microscopy (AFM), we found that adhesion force of an exoskeletal (presumably molting) fluid decreased almost five-fold from 88 to 17 nN within an hour of molting. Our findings may have implications for laboratory safety and the effectiveness of bed bug traps, barriers, and biomimetic-based adhesives

Adhesiveness of exoskeletal fluid measured in nN of force required to pull the probe cantilever from the surface of the bed bug exoskeleton.

<p>Error bars represent standard deviation. Comparisons significant at the 0.05 level are indicated by (*).</p

Change in adhesion force in nN measured over time after molting to adulthood.

<p>Error bars represent standard deviation.</p