Adsorption Behavior of Iodine by Novel Covalent Organic Polymers Constructed Through Heterostructural Mixed Linkers

The efficient capture and storage radioactive iodine (129I or 131I) formed during the extensive use of nuclear energy is of paramount importance. Therefore, it is a great deal to design and empolder new adsorbents for effectively disposing of iodine from nuclear waste. In this work, we presented a novel covalent organic polymer (JLUE-COP-3) constructed through heterostructural mixed linkers with perforated porousness, plenty of π-conjugated phenyl rings and functional –CO–NH– and –SO3H groups to iodine adsorption process. After fully characterizing the morphology and structure, the adsorption behavior of iodine by the resultant polymers were explored in detail. The external adsorption behavior was determined to obey the pseudo-second order kinetic model according to the kinetic analysis. The maximum liquid adsorption capacity was obtained to reach 153.85 mg/g at 298 K, which was evaluated by the Langmuir isotherm model. In addition, the negative attributes of ΔG° as well as the positive attributes of ΔH° and ΔS° at three temperatures indicated the nature of the iodine adsorption over JLUE-COP-3 was spontaneous and endothermic. The current study could look forward to making great contributions to the facile fabrication of late-model three-component POP materials and their applications in treatment of nuclear waste

All-Optical Switching Based on Sub-Bandgap Photoactivation of Charge Trapping in Metal Halide Perovskites

Controllable optical properties are crucial for the application of light-emitting materials in optical devices. In this work, controllable photoluminescence in metal halide perovskite crystals is realized via photoactivation of their defects. It is found that under continuous excitation, the photoluminescence intensity of a CH3NH3PbBr3 crystal can be fully controlled by sub-bandgap energy photon illumination. Such optically controllable emission behavior is rather general as it is observed also in CsPbBr3 and other perovskite materials. The switching mechanism is assigned to reversible light-induced activation/deactivation of nonradiative recombination centers, the presence of which relates to an excess of Pb during perovskite synthesis. Given the success of perovskites in photovoltaics and optoelectronics, it is believed that the discovery of green luminescence controlled by red illumination will extend the application scope of perovskites toward optical devices and intelligent control

Evaluation of the Mucosal Immunity Effect of Bovine Viral Diarrhea Virus Subunit Vaccine E2Fc and E2Ft

Classified as a class B infectious disease by the World Organization for Animal Health (OIE), bovine viral diarrhea/mucosal disease is an acute, highly contagious disease caused by the bovine viral diarrhea virus (BVDV). Sporadic endemics of BVDV often lead to huge economic losses to the dairy and beef industries. To shed light on the prevention and control of BVDV, we developed two novel subunit vaccines by expressing bovine viral diarrhea virus E2 fusion recombinant proteins (E2Fc and E2Ft) through suspended HEK293 cells. We also evaluated the immune effects of the vaccines. The results showed that both subunit vaccines induced an intense mucosal immune response in calves. Mechanistically, E2Fc bonded to the Fc γ receptor (FcγRI) on antigen-presenting cells (APCs) and promoted IgA secretion, leading to a stronger T-cell immune response (Th1 type). The neutralizing antibody titer stimulated by the mucosal-immunized E2Fc subunit vaccine reached 1:64, which was higher than that of the E2Ft subunit vaccine and that of the intramuscular inactivated vaccine. The two novel subunit vaccines for mucosal immunity developed in this study, E2Fc and E2Ft, can be further used as new strategies to control BVDV by enhancing cellular and humoral immunity

Transportin-3 Facilitates Uncoating of Influenza A Virus

Influenza A viruses (IAVs) are a major global health threat and in the future, may cause the next pandemic. Although studies have partly uncovered the molecular mechanism of IAV–host interaction, it requires further research. In this study, we explored the roles of transportin-3 (TNPO3) in IAV infection. We found that TNPO3-deficient cells inhibited infection with four different IAV strains, whereas restoration of TNPO3 expression in knockout (KO) cells restored IAV infection. TNPO3 overexpression in wild-type (WT) cells promoted IAV infection, suggesting that TNPO3 is involved in the IAV replication. Furthermore, we found that TNPO3 depletion restrained the uncoating in the IAV life cycle, thereby inhibiting the process of viral ribonucleoprotein (vRNP) entry into the nucleus. However, KO of TNPO3 did not affect the virus attachment, endocytosis, or endosomal acidification processes. Subsequently, we found that TNPO3 can colocalize and interact with viral proteins M1 and M2. Taken together, the depletion of TNPO3 inhibits IAV uncoating, thereby inhibiting IAV replication. Our study provides new insights and potential therapeutic targets for unraveling the mechanism of IAV replication and treating influenza disease

Identification of a New Rice Blast Resistance Gene, Pid3, by Genomewide Comparison of Paired Nucleotide-Binding Site–Leucine-Rich Repeat Genes and Their Pseudogene Alleles Between the Two Sequenced Rice Genomes

Rice blast, caused by Magnaporthe oryzae, is one of the most devastating diseases. The two major subspecies of Asian cultivated rice (Oryza sativa L.), indica and japonica, have shown obvious differences in rice blast resistance, but the genomic basis that underlies the difference is not clear. We performed a genomewide comparison of the major class of resistant gene family, the nucleotide-binding site–leucine-rich repeat (NBS–LRR) gene family, between 93-11 (indica) and Nipponbare (japonica) with a focus on their pseudogene members. We found great differences in either constitution or distribution of pseudogenes between the two genomes. According to this comparison, we designed the PCR-based molecular markers specific to the Nipponbare NBS–LRR pseudogene alleles and used them as cosegregation markers for blast susceptibility in a segregation population from a cross between a rice blast-resistant indica variety and a susceptible japonica variety. Through this approach, we identified a new blast resistance gene, Pid3, in the indica variety, Digu. The allelic Pid3 loci in most of the tested japonica varieties were identified as pseudogenes due to a nonsense mutation at the nucleotide position 2208 starting from the translation initiation site. However, this mutation was not found in any of the tested indica varieties, African cultivated rice varieties, or AA genome-containing wild rice species. These results suggest that the pseudogenization of Pid3 in japonica occurred after the divergence of indica and japonica