770 research outputs found

    On the size of knots in ring polymers

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    We give two different, statistically consistent definitions of the length l of a prime knot tied into a polymer ring. In the good solvent regime the polymer is modelled by a self avoiding polygon of N steps on cubic lattice and l is the number of steps over which the knot ``spreads'' in a given configuration. An analysis of extensive Monte Carlo data in equilibrium shows that the probability distribution of l as a function of N obeys a scaling of the form p(l,N) ~ l^(-c) f(l/N^D), with c ~ 1.25 and D ~ 1. Both D and c could be independent of knot type. As a consequence, the knot is weakly localized, i.e. ~ N^t, with t=2-c ~ 0.75. For a ring with fixed knot type, weak localization implies the existence of a peculiar characteristic length l^(nu) ~ N^(t nu). In the scaling ~ N^(nu) (nu ~0.58) of the radius of gyration of the whole ring, this length determines a leading power law correction which is much stronger than that found in the case of unrestricted topology. The existence of such correction is confirmed by an analysis of extensive Monte Carlo data for the radius of gyration. The collapsed regime is studied by introducing in the model sufficiently strong attractive interactions for nearest neighbor sites visited by the self-avoiding polygon. In this regime knot length determinations can be based on the entropic competition between two knotted loops separated by a slip link. These measurements enable us to conclude that each knot is delocalized (t ~ 1).Comment: 29 pages, 14 figure

    Alterações morfológicas e qualidade fisiológica de sementes de mamão.

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    A análise de raios X é uma técnica importante na identificação de problemas associados à morfologia interna de sementes e ao seu potencial fisiológico. O objetivo do estudo foi avaliar os padrões morfológicos e a qualidade fisiológica de sementes de mamão obtidas de diferentes estádios de maturação e locais dentro do fruto com o auxílio das análises de raios X. Foram utilizadas sementes de mamão Formosa colhidos no estádio 1 de maturação. As sementes foram extraídas dos frutos nos estádios 1, 3, 5 e final. Os frutos foram armazenados em condições de laboratório (25 °C) e ao atingirem cada estádio de maturação, foram seccionados transversalmente em três partes de tamanhos iguais, sendo as sementes das duas extremidades (proximal e distal) extraídas separadamente daquelas localizadas na região central. Depois de lavadas e secas, as sementes foram utilizadas nas seguintes avaliações: 1) teste de germinação em papel germitest umedecido com água destilada em temperatura alternada 20-30°C. Avaliou-se a velocidade de germinação, plântulas normais na primeira contagem e as porcentagens de germinação, sementes dormentes e sementes inviáveis aos 30 dias; e, 2) teste de raios X, determinando-se as porcentagens de sementes cujo embrião era facilmente visível, com radícula visível e sementes aparentemente vazias. O experimento foi conduzido em delineamento inteiramente casualizado, em fatorial (duas regiões do fruto e quatro estádios de maturação) em quatro repetições. As médias comparadas pelo teste de Tukey a 5% de probabilidade. Constatou-se que sementes localizadas na parte central dos frutos apresentaram desempenho superior às sementes das extremidades. O estádio 1 de maturação apresentou número de sementes vazias e dormentes superior aos demais estádios. O teste de raios X favoreceu a diferenciação entre sementes de mamão vazias e com embrião completamente desenvolvido.Resum

    Maternal neurofascin-specific autoantibodies bind to structures of the fetal nervous system during pregnancy, but have no long term effect on development in the rat

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    Neurofascin was recently reported as a target for axopathic autoantibodies in patients with multiple sclerosis (MS), a response that will exacerbate axonal pathology and disease severity in an animal model of multiple sclerosis. As transplacental transfer of maternal autoantibodies can permanently damage the developing nervous system we investigated whether intrauterine exposure to this neurofascin-specific response had any detrimental effect on white matter tract development. To address this question we intravenously injected pregnant rats with either a pathogenic anti-neurofascin monoclonal antibody or an appropriate isotype control on days 15 and 18 of pregnancy, respectively, to mimic the physiological concentration of maternal antibodies in the circulation of the fetus towards the end of pregnancy. Pups were monitored daily with respect to litter size, birth weight, growth and motor development. Histological studies were performed on E20 embryos and pups sacrificed on days 2, 10, 21, 32 and 45 days post partum. Results: Immunohistochemistry for light and confocal microscopy confirmed passively transferred anti-neurofascin antibody had crossed the placenta to bind to distinct structures in the developing cortex and cerebellum. However, this did not result in any significant differences in litter size, birth weight, or general physical development between litters from control mothers or those treated with the neurofascin-specific antibody. Histological analysis also failed to identify any neuronal or white matter tract abnormalities induced by the neurofascin-specific antibody. Conclusions: We show that transplacental transfer of circulating anti-neurofascin antibodies can occur and targets specific structures in the CNS of the developing fetus. However, this did not result in any pre- or post-natal abnormalities in the offspring of the treated mothers. These results assure that even if anti-neurofascin responses are detected in pregnant women with multiple sclerosis these are unlikely to have a negative effect on their children

    Prestressed concrete bridge tested to failure : the Alveo Vecchio viaduct case study

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    The number of bridges approaching or exceeding their initial design life has been increasing dramatically. Meanwhile, bridges are withstanding an ever-increasing traffic volume, both in number and weight of vehicles. Analytical and numerical models can predict bridges’ response to traffic loads and their ultimate capacity with low uncertainties; however, such uncertainties increase as bridges age due to deterioration mechanisms. Non-destructive tests of material specimens and full-scale load tests allow for updating structural models and predicting bridges’ responses with higher accuracy. On-site load tests with different load levels provide different information on the bridge behaviour (e.g., elastic response, first-crack load, and ultimate capacity), which impact the model updating differently. This paper compares the observed response of the Alveo Vecchio viaduct, a prestressed concrete (PC) bridge subjected to a controlled load test up to its failure, with its predicted response provided by four structural models. The observed response is measured by an extensive structural health monitoring system, while the structural models are developed with different levels of refinement and uncertainty in the input parameters. This study gives an insight into the ultimate load-carrying capacity of existing PC bridges and their behaviour during a whole load test to failure. The results show that the load-carry capacity of the Alveo Vecchio viaduct is almost four times higher than the design load; likely, many other Italian highway bridges with similar structural characteristics have a similar capacity

    Teores de Fósforo em Plantas de Pinhão Manso submetido a doses de Alumínio em cultivo Hidropônico.

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    O pinhão manso, Jatropha curcas L., pertence à família Euphorbiaceae e é uma espécie amplamente distribuída em áreas tropicais e subtropicais e com potencial para a produção de biocombustível. O objetivo desse trabalho foi avaliar os teores de fósforo em diferentes acessos de pinhão manso cultivados em solução hidropônica com doses crescentes de alumínio. Foi implantado um experimento na UFRRJ, em Seropédica, na casa de vegetação localizada na área experimental do Instituto de Agronomia em solução nutritiva, seguindo um delineamento blocos casualizados com três repetições, num esquema fatorial 4 x 5. Os tratamentos corresponderam a quatro acessos, sendo dois com alto teor e dois com baixo teor de éster de forbol e cinco doses de alumínio: 0,0; 12,5; 25,0; 50,0 e 100,0 μM L-1. Cada unidade experimental consistiu de um vaso contendo duas plantas. Após 10 dias da aplicação dos tratamentos, as plantas foram coletadas e seccionadas em raiz, caule, pecíolo e folha, sendo posteriormente levados à estufa de circulação de ar forçada a 650C por 72 horas até atingir peso constante. Após secagem e digestão nítrica do material vegetal, foram determinados os teores de P. Os acessos UFRRJ apresentaram os maiores teores de P na folha, pecíolo, caule e raiz em relação aos acessos do CNPAE. O aumento das doses de alumínio reduz os teores de fósforo nas partes vegetativas do pinhão manso
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