Effects of Naringin on Proliferation and Osteogenic Differentiation of Human Periodontal Ligament Stem Cells In Vitro and In Vivo

This study is to explore the osteogenesis potential of the human periodontal ligament stem cells (hPDLSCs) induced by naringin in vitro and in vitro. The results confirmed that 1 μM naringin performs the best effect and a collection of bone-related genes (RUNX2, COL1A2, OPN, and OCN) had significantly higher expression levels compared to the control group. Furthermore, a typical trabecular structure was observed in vivo, surrounded by a large amount of osteoblasts. These results demonstrated that naringin, at a concentration of 1 μM, can efficiently promote the proliferation and differentiation of hPDLSCs both in vitro and in vivo

Design of and Experiment on a Cleaning Mechanism of the Pneumatic Single Seed Metering Device for Coated Hybrid Rice

In order to improve the single-grain seeding rate of the pneumatic single seed metering device, an airflow seed cleaning device was designed in combination with positive pressure airflow. The influence of the position of the seed cleaning mechanism on the seed cleaning effect is theoretically analyzed and a flow field simulation test analysis of different nozzle structures was carried out by using Fluent software (ANSYS, Inc., Canonsburg, PA, USA). The results of this test show that a nozzle with a Witoszynski curve has good airflow concentration and uniform air pressure distribution. In order to verify the performance of the seed cleaning mechanism, a 0.7 times coated seed (hybrid rice Wuyou 1179) was used as the test material and a quadratic regression test with three levels was carried out with the rotation speed of the seed plate, the negative pressure of the suction chamber, and the positive pressure of the seed cleaning as the test factors. The results showed that when the speed of sucking plate was 30 r/min, the negative pressure of the suction chamber was 1.8 kPa and the positive pressure of the seed cleaning was 0.2 kPa; the seeding effect was at its best and the qualified rate of the seed metering device was the highest at 86.43%, the minimum leakage rate was 3.81%, and the multiple rate was 9.76%. The proposed seed cleaning mechanism effectively improves the accuracy of seeding and provides a certain theoretical basis for the single-grain sowing of hybrid rice