Nonequilibrium Relaxation of Conformational Dynamics Facilitates Catalytic Reaction in an Elastic Network Model of T7 DNA Polymerase

10.1021/acs.jpcb.5b11002The Journal of Physical Chemistry B120112869-287

In vivo imaging of single mammalian cells in development and disease

Live imaging has transformed biomedical sciences by enabling visualization and analysis of dynamic cellular processes as they occur in their native contexts. Here, we review key recent efforts applying in vivo optical imaging with single-cell resolution to mammalian systems ranging from embryos to adult tissues and organs. We highlight insights into active processes regulating cell fate and morphogenesis during embryonic development, how neuronal circuitry and non-neuronal cell types contribute to neurological functions, and how novel imaging-based approaches enable the dissection of neurological disorders and cancer with high spatio-temporal resolution. The convergence of technical advancements in accessing, visualizing, and manipulating individual cells provides an unprecedented lens to probe mammalian cellular dynamics in vivo in both physiological and pathological states

In Vivo Imaging of Single Mammalian Cells in Development and Disease

10.1016/j.molmed.2018.01.003Trends in Molecular Medicine243278-29

Quantitative imaging of mammalian transcriptional dynamics: from single cells to whole embryos

10.1186/s12915-016-0331-9BMC Biology14

Quantitative imaging of mammalian transcriptional dynamics: from single cells to whole embryos

Probing dynamic processes occurring within the cell nucleus at the quantitative level has long been a challenge in mammalian biology. Advances in bio-imaging techniques over the past decade have enabled us to directly visualize nuclear processes in situ with unprecedented spatial and temporal resolution and single-molecule sensitivity. Here, using transcription as our primary focus, we survey recent imaging studies that specifically emphasize the quantitative understanding of nuclear dynamics in both time and space. These analyses not only inform on previously hidden physical parameters and mechanistic details, but also reveal a hierarchical organizational landscape for coordinating a wide range of transcriptional processes shared by mammalian systems of varying complexity, from single cells to whole embryos

Reply to "Convergence of chromatin binding estimates in live cells"

10.1038/nmeth.2574Nature Methods108692-69

Dynamic Fluctuations in Subcellular Localization of the Hippo Pathway Effector Yorkie In Vivo

10.1016/j.cub.2018.04.018Current Biology28101651-1660.e

Single-molecule imaging of transcription factor binding to DNA in live mammalian cells

10.1038/nmeth.2411Nature Methods105421-42