Molecular responses to salinity stress in Salix matsudana (Koidz) females and males

Sexual dimorphism has commonly been found in many species. The phenotypes of Salix matsudana females and males are different under salinity stress. An F1 population was selected to compare the differences between males and females. As a result, males showed stronger roots and heavier dry weights than females. The unique molecular mechanisms of males and females under salinity stress were further analyzed based on the root transcriptome of males and females. Both males and females up-regulated systemic acquired resistance genes, such as ADH and oxygenase-related genes, to resist salt. Moreover, many other abiotic stress response genes were up-regulated in males to adjust to salinity stress, while females showed more down-regulation of nitrogen metabolism-related genes to decrease the harm from salinity stress. The research on salinity tolerance in Salix matsudana males and females would help to further understand sexual dimorphism under selection pressure and provide benefits to the ecological environment

Integrating network pharmacology and experimental studies for uncovering the molecular mechanisms of Dioscorea bulbifera L. in the treatment of thyroid cancer

Introduction: Thyroid cancer (TC), a common endocrine malignant tumor with a higher incidence in females than in males, is a serious threat to human life and health. Although current clinical treatments can alleviate this disease, the recurrence rate remains high. Tuber Dioscoreae Bulbiferae, also called Huang-Yao-Zi (HYZ), has remarkable curative properties, few side effects and is used for the treatment of sore throat, goiter, hemoptysis, and other diseases in traditional Chinese medicine (TCM). Existing clinical studies have found that HYZ can improve the clinical symptoms of TC patients and reduce tumor volume, while in vitro studies have found that HYZ can induce the death of thyroid cancer cells. However, the mechanism of HYZ in the treatment of TC is still unclear. Methods: In this study, based on network pharmacology and bioinformatics, the target and molecular mechanism of HYZ in the treatment of TC were preliminarily explored. The results suggest that the antithyroid cancer effect of HYZ may be related to the PI3K-Akt and focal adhesion pathways. Then, a TC cell model was established to further explore the detail molecular mechanisms. Results: Fortunately, HYZ induced apoptosis in KMH-2 cells and regulated the expression of apoptosis-related proteins and genes. At the same time, HYZ can also significantly inhibit the migration and invasion of TC cells. Further studies showed that the pharmacological activities of HYZ were related to the regulation of the PI3K-Akt and focal adhesion pathways. Conclusion: Our study provides a reference for further animal or clinical studies investigating the effectiveness and molecular mechanisms of HYZ against thyroid cancer

Calculation of GTFP and its influencing factors in Guangdong-Hong Kong-Macao Greater Bay Area

Under the background of dual carbon target, the manufacturing industry in the Guangdong-Hong Kong-Macao Greater Bay Area (GBA) is facing the pressure of green transformation. This paper takes this as the research basis, collects the production panel data of the manufacturing industry in the GBA in the past decade, calculates the green development level of the GBA and finds out the important influencing factors, so as to provide theoretical suggestions for the green production and green development of the manufacturing industry in the GBA

Mechanical Properties of Ultra-High Performance Concrete with Coal Gasification Coarse Slag as River Sand Replacement

Coal gasification coarse slag (CGCS) is a by-product of coal gasification. Despite its abundance, CGCS is mostly used in boiler blending, stacking, and landfill. Large-scale industrial applications of CGCS can be environment-friendly and cost saving. In this study, the application of CGCS as a substitute for river sand (RS) with different replacement ratios in ultra-high performance concrete (UHPC) was investigated. The effects of CGCS replacement ratios on the fluidity and mechanical properties of specimens were examined, and the effect mechanisms were explored on the basis of hydration products and the multi-scale (millimetre-scale and micrometre-scale) microstructure analysis obtained through X-ray diffraction (XRD), scanning electron microscopy, and X-ray energy-dispersive spectroscopy. With an increase in the CGCS replacement ratio, the water–binder ratio (w/b), flexural strength, and compressive strength decreased. Specimens containing CGCS of ≤25% can satisfy the strength requirement of non-structural UHPC, with flexure strength of 29 MPa and compressive strength of 111 MPa at day 28. According to the XRD results and multi-scale microstructure analysis, amorphous glass beads in CGCS positively influenced ettringite generation due to the pozzolanic activity. Porous carbon particles in CGCS showed strong interfacial bonding with cement slurry due to internal hydration; this bonding was conducive to improving the mechanical strength. However, CGCS hindered hydration in the later curing stage, leading to an increase in the unreacted cement and agglomeration of fly ash; in addition, at a CGCS replacement ratio of up to 50%, an apparent interfacial transition zone structure was observed, which was the main contributor to mechanical strength deterioration

Relationships among shift work, hair cortisol concentration and sleep disorders: a cross-sectional study in China

Objective The present study was designed to demonstrate the relationships among shift work, hair cortisol concentration (HCC) and sleep disorders.Design A cross-sectional study.Setting Three petroleum administrations in Karamay city of Xinjiang, China.Participants 435 individuals including 164 males and 271 females participated in the research.Outcome measures Information on shift work was collected by a self-administered questionnaire. HCC was determined using an automatic radioimmunoassay instrument. Sleep quality was measured on the Pittsburgh Sleep Quality Index scale.Results Shiftwork was associated with an increased prevalence of sleep disorders compared with the fixed day shift (two shifts: OR 3.11, 95% CI 1.57 to 6.19; three shifts: OR 2.87, 95% CI 1.38 to 5.98; four shifts: OR 2.22, 95% CI 1.17 to 4.18; others: OR 3.88, 95% CI= 1.36 to 11.08). Workers with different shift patterns had higher HCC levels than day workers ((fixed day shift: geometric mean±geometric SD=2.33±1.65; two shifts: 3.76±1.47; three shifts: 3.15±1.64; four shifts: 3.81±1.55; others: 3.60±1.33) ng/g hair, η2=0.174) and high HCC was associated with the higher prevalence of sleep disorders (OR 4.46, 95% CI 2.70 to 7.35). The mediating effect of HCC on the relationship between shift work and sleep disorders was 0.25 (95% CI 0.09 to 0.41).Conclusion We found that, when compared with the fixed day shift, shiftwork was associated with both the higher HCC, and also with an increased risk of sleep disorders. High HCC was associated with the occurrence of sleep disorders. In addition, HCC had mediating effect in shift work and sleep disorders. Thus, HCC can be considered as an early marker of shiftwork circadian disruption to early detection and management of sleep disorders

A cohort study on the association between changing occupational stress, hair cortisol concentration, and hypertension.

ObjectiveTo explore the relationship between changing occupational stress levels, hair cortisol concentration (HCC), and hypertension.MethodsBaseline blood pressure of 2520 workers was measured in 2015. The Occupational Stress Inventory-Revised Edition (OSI-R) was used to assess changes in occupational stress. Occupational stress and blood pressure were followed up annually from January 2016 to December 2017. The final cohort numbered 1784 workers. The mean age of the cohort was 37.77±7.53 years and the percentage male was 46.52%. At baseline, 423 eligible subjects were randomly selected for hair sample collection to determine cortisol levels.ResultsIncreased occupational stress was a risk factor for hypertension [risk ratio (RR) = 4.200, 95% confidence interval (CI): 1.734-10.172]. The HCC of workers with elevated occupational stress was higher than that of workers with constant occupational stress [(ORQ score ≥70: geometric mean±geometric standard deviation = 5.25±3.59 ng/g hair; 60-90: 5.02±4.00; 40-59: 3.45±3.41; ConclusionsIncreased occupational stress could lead to an increase in hypertension incidence. High HCC could increase the risk of hypertension. HCC acts as a mediator between occupational stress and hypertension

Screening of Lactic Acid Bacteria Suitable for the Fermentation of Shenheling Slimming Beverages Based on the Activity Inhibition of Energy Digestive Enzymes and a Sensory Evaluation

Obesity is a prevalent chronic disease worldwide. In this study, we screened lactic acid bacteria (LAB) suitable for fermenting Shenheling extract (SHLE) to enhance its anti-obesity efficacy and improve flavor. Using SHLE as the medium, a single strain was inoculated and the lactic acid bacteria suitable for growth in SHLE were preliminarily screened through a growth curve. The growth of the initially screened LAB was characterized in detail by the pH value, titration acidity and viable bacteria count. At the same time, appropriate LAB were selected with the lipase activity inhibition rate, α-glucosidase activity inhibition rate and a sensory evaluation as the response indicators. As a result, 6 of the 12 strains of lactic acid bacteria grew well in SHLE. The fermentation of five representative LAB could significantly improve the inhibition rate of the lipase activity of SHLE and maintain the inhibition rate of the α-glucosidase activity at a high level. In addition, fermentation removed the original flavors of SHLE such as grass, bitterness and cassia and added a sour taste, fruity aroma and cool taste. Among them, Lactobacillus fermentum grx08 and Lactobacillus rhamnosus hsryfm1301 gave SHLE a soft sour taste after fermentation. L. fermentum grx08, L. rhamnosus grx10 and hsryfm1301 imparted a moderately fruity aroma to SHLE after fermentation. In summary, L. fermentum grx08 and L. rhamnosus hsryfm1301 were the candidate strains for fermenting SHLE to produce good-flavored slimming functional drinks

Straw Incorporation Management Affects Maize Grain Yield through Regulating Nitrogen Uptake, Water Use Efficiency, and Root Distribution

Returning crop straw to soil can potentially improve soil health and crop production, facilitating sustainable agriculture. However, the effects of straw incorporation with various tillage management techniques combined with nitrogen (N) regimes on crop root growth, and water and N utility are not well understood. In this study, rotary tillage (RTS) and plow tillage (PTS) for straw incorporation combined with N regimes (CK, no N applied; LN, 112 kg N ha−1; MN, 187 kg N ha−1; and HN, 262 kg N ha−1) were used to determine their effects on soil water and mineral N availability, root distribution, crop N uptake, grain yield, and water use efficiency (WUE) of maize in northeast China. The results demonstrate that higher levels of pre-sowing soil-water storage and field evapotranspiration (ET), and lower levels of WUE and pre-sowing soil mineral N storage (Nmin-PS) at a depth of 0–60 cm were obtained with the RTS treatments as compared to the PTS treatments. N addition improved Nmin-PS and post-harvest soil mineral N storage (Nmin-PH) at a depth of 60–100 cm in 2016, and increased WUE compared to CK. RTS treatments enhanced root weight density (RWD) at a depth of 0–60 cm in 2016–2017, root length density (RLD), ratio of root length density (RLDR), and ratio of root weight density (RWDR) at a depth of 30–60 cm in 2016, and RLD at a depth of 0–30 cm in 2017. N addition promoted RLD and RWD at a depth of 0–10 cm in 2016–2017. RTS treatments reduced pre-silking shoot N uptake (NPS) and grain yield. Shoot N uptake and grain yield were enhanced in response to increasing levels of N; however, the grain yield did not show further significant improvements when the amount of N applied was over 187 kg N ha−1 (except for RTS in 2016). Overall, tillage with straw incorporation management and N levels markedly affected the soil physicochemical properties (such as ET, Nmin-PS, and Nmin-PH). This influenced grain yield indirectly by further mediating root traits (RLD, RWD, RLDR, and RWDR) with consequences for the NPS and post-silking shoot N uptake (NPOS) of maize, which were found to have greatest direct and positive impact on maize grain yield

Safety of low-dose aspirin in endovascular treatment for intracranial atherosclerotic stenosis.

OBJECTIVES: To evaluate the safety of low-dose aspirin plus clopidogrel versus high-dose aspirin plus clopidogrel in prevention of vascular risk within 90 days of duration of dual antiplatelet therapy in patients treated with intracranial endovascular treatment. METHODS: From January 2012 to December 2013, this prospective and observational study enrolled 370 patients with symptomatic intracranial atherosclerotic stenosis of ≥70% with poor collateral undergoing intracranial endovascular treatment. Antiplatelet therapy consists of aspirin, at a low-dose of 100 mg or high-dose of 300 mg daily; clopidogrel, at a dose of 75 mg daily for 5 days before endovascular treatment. The dual antiplatelet therapy continued for 90 days after intervention. The study endpoints include acute thrombosis, subacute thrombosis, stroke or death within 90 days after intervention. RESULTS: Two hundred and seventy three patients received low-dose aspirin plus clopidogrel and 97 patients received high-dose aspirin plus clopidogrel before intracranial endovascular treatment. Within 90 days after intervention, there were 4 patients (1.5%) with acute thrombosis, 5 patients (1.8%) with subacute thrombosis, 17 patients (6.2%) with stroke, and 2 death (0.7%) in low-dose aspirin group, compared with no patient (0%) with acute thrombosis, 2 patient (2.1%) with subacute thrombosis, 6 patients (6.2%) with stroke, and 2 death (2.1%) in high-dose aspirin group, and there were no significant difference in all study endpoints between two groups. CONCLUSION: Low-dose aspirin plus clopidogrel is comparative in safety with high-dose aspirin plus clopidogrel within 90 days of duration of dual antiplatelet therapy in patients treated with intracranial endovascular treatment

Sulforaphane alleviates psoriasis by enhancing antioxidant defense through KEAP1-NRF2 Pathway activation and attenuating inflammatory signaling

Abstract Psoriasis is a chronic inflammatory skin disease that affects millions of people worldwide. Sulforaphane (SFN) has been shown to have anti-inflammatory and antioxidant properties. In this study, we investigated the effects of SFN on a mouse model of psoriasis induced by imiquimod (IMQ) and its underlying molecular mechanism. Mice treated with SFN showed significant improvement in psoriatic symptoms, including reduced erythema, scales, and cutaneous thickness. Histopathological analysis and immunohistochemical staining revealed decreased expression of K16, K17, and Ki67 in SFN-treated mice, indicating reduced abnormal differentiation of keratinocytes and cutaneous inflammation. SFN treatment also reduced the activation of STAT3 and NF-κB pathways and downregulated pro-inflammatory cytokines IL-1β, IL-6, and CCL2. In vitro experiments using HaCaT cells demonstrated that SFN inhibited IL-22 and TNF-α-induced activation of inflammatory pathways and keratinocyte proliferation. Network pharmacology analysis suggested that the KEAP1-NRF2 pathway might be involved in the protective effects of SFN on psoriasis. We observed reduced NRF2 expression in human psoriatic lesions, and subsequent experiments showed that SFN activated KEAP1-NRF2 pathway in vivo and in vitro. Importantly, NRF2-deficient mice exhibited aggravated psoriasis-like symptoms and reduced response to SFN treatment. Our findings indicate that SFN ameliorates psoriasis symptoms and inflammation through the KEAP1-NRF2 pathway, suggesting a potential therapeutic role for SFN in the treatment of psoriasis