Multi-frequency Studies of Massive Cores with Complex Spatial and Kinematic Structures

Five regions of massive star formation have been observed in various molecular lines in the frequency range $\sim 85-89$ GHz. The studied regions possess dense cores, which host young stellar objects. The physical parameters of the cores are estimated, including kinetic temperatures ($\sim 20-40$ K), sizes of the emitting regions ($\sim 0.1-0.6$ pc), and virial masses ($\sim 40-500 M_{\odot}$). Column densities and abundances of various molecules are calculated in the local thermodynamical equilibrium approximation. The core in 99.982+4.17, associated with the weakest IRAS source, is characterized by reduced molecular abundances. Molecular line widths decrease with increasing distance from the core centers ($b$). For b\ga 0.1~pc, the dependences $\Delta V(b)$ are close to power laws ($\propto b^{-p}$), where $p$ varies from $\sim 0.2$ to $\sim 0.5$, depending on the object. In four cores, the asymmetries of the optically thick HCN(1--0) and HCO$^+$(1--0) lines indicate systematic motions along the line of sight: collapse in two cores and expansion in two others. Approximate estimates of the accretion rates in the collapsing cores indicate that the forming stars have masses exceeding the solar mass.Comment: 18 pages, 7 figures, 6 table

PROPHYLAXIS OF «ATYPICAL PNEUMONIA» AT TOMSKAYA PROVINCE

Materials of the organizing and methodical activities performed by the specialists of State Surveillance Center at Tomskaya Province, Public Health Department, Departmental Services with the support of the Province administration to prevent importation and dissemination of «atypical pneumonia» are represented

Senescent vs. non-senescent cells in the human annulus in vivo: Cell harvest with laser capture microdissection and gene expression studies with microarray analysis

<p>Abstract</p> <p>Background</p> <p>Senescent cells are well-recognized in the aging/degenerating human disc. Senescent cells are viable, cannot divide, remain metabolically active and accumulate within the disc over time. Molecular analysis of senescent cells in tissue offers a special challenge since there are no cell surface markers for senescence which would let one use fluorescence-activated cell sorting as a method for separating out senescent cells.</p> <p>Methods</p> <p>We employed a novel laser capture microdissection (LCM) design to selectively harvest senescent and non-senescent annulus cells in paraffin-embedded tissue, and compared their gene expression with microarray analysis. LCM was used to separately harvest senescent and non-senescent cells from 11 human annulus specimens.</p> <p>Results</p> <p>Microarray analysis revealed significant differences in expression levels in senescent cells vs non-senescent cells: 292 genes were upregulated, and 321 downregulated. Genes with established relationships to senescence were found to be significantly upregulated in senescent cells vs. non-senescent cells: p38 (MPAK14), RB-Associated KRAB zinc finger, Discoidin, CUB and LCCL domain, growth arrest and DNA-damage inducible beta, p28ING5, sphingosine-1-phosphate receptor 2 and somatostatin receptor 3; cyclin-dependent kinase 8 showed significant downregulation in senescent cells. Nitric oxidase synthase 1, and heat shock 70 kDa protein 6, both of which were significantly down-regulated in senescent cells, also showed significant changes. Additional genes related to cytokines, cell proliferation, and other processes were also identified.</p> <p>Conclusions</p> <p>Our LCM-microarray analyses identified a set of genes associated with senescence which were significantly upregulated in senescent vs non-senescent cells in the human annulus. These genes include p38 MAP kinase, discoidin, inhibitor of growth family member 5, and growth arrest and DNA-damage-inducible beta. Other genes, including genes associated with cell proliferation, extracellular matrix formation, cell signaling and other cell functions also showed significant modulation in senescent vs non-senescent cells. The aging/degenerating disc undergoes a well-recognized loss of cells; understanding senescent cells is important since their presence further reduces the disc's ability to generate new cells to replace those lost to necrosis or apoptosis.</p

Modern state of irrigated soils at the south of the Volga upland

The goal is to assess the impact of 50-year irrigation by sprinkling on soil processes occurring in the light-chestnut soils (Luvic Kastanozem (Loamic, Aric, Protosodic, Bathysalic)) of the southern slopes of the Volga upland at the Volga-Don interfluve (FSUE “Oroshaemoe”, the Volgograd region) with deep ground water. Water for irrigation is supplied from the Varvarovsky reservoir of the Volga-Don Canal system. It is characterized by a total dissolved salts of about 1 g/l, a bicarbonate-chloride-sulfate compositionwith an increased sodium content. Detailed morphological description of soil profiles, granulometric composition, content of soluble salts in soils and sediments of the vadoze zone up to the depth of 3.5 m, dynamics of salts in the layer of 0-50 cm for 2011-2019 are presented. Until the autumn of 2015, the studied soils were deep saline, being no saline in the layer of 0-100 cm. In recent years, a weak salinity degree of soda-chloride sodium chemistry has been observed in the 0-50 cm layer as a result of gradual accumulation of irrigation water salts during irrigation organized according to water consumption of agricultural crops. Irrigated soils have acquired a complex of signs of secondary salinity: (1) the presence of light accumulations of sandy and silt mineral grains in the arable horizon, resulting from the destructive effect of irrigation water drops during sprinkling; (2) toxic alkalinity associated with sodium (residual sodium carbonate), according to water extraction 1 : 5 (soil : water), in the horizons from the depth of 10-20 to 60100 cm; (3) abundant humus-clay cutans on the lateral side faces of prismatic structural units in the undisturbed part of the soil profile from 30 to 100 cm

Fragmentation and dynamics of dense gas structures in the proximity of massive young stellar object W42-MME

We present an analysis of the dense gas structures in the immediate surroundings of the massive young stellar object (MYSO) W42-MME, using the high-resolution (0$''$.31$\times$0$''$.25) ALMA dust continuum and molecular line data. We performed a dendrogram analysis of H$^{13}$CO$^{+}$ (4-3) line data to study multi-scale structures and their spatio-kinematic properties, and analyzed the fragmentation and dynamics of dense structures down to $\sim$2000 AU scale. Our results reveal 19 dense gas structures, out of which 12 are leaves and 7 are branches in dendrogram terminology. These structures exhibit transonic-supersonic gas motions (1$<\mathcal{M}<5$) with overvirial states ($\alpha_{\rm vir}\geq2$). The non-thermal velocity dispersion-size relation ($\sigma_{\rm nt}-L$) of dendrogram structures shows a weak negative correlation, while the velocity dispersion across the sky ($\delta\mathit{V_{\rm lsr}}$) correlates positively with structure size ($L$). Velocity structure function ($S_{2}(l)^{1/2}$) analysis of H$^{13}$CO$^{+}$ data reveals strong power-law dependencies with lag ($l$) up to a scale length of $\lesssim$ 6000 AU. The mass-size ($M-R$) relation of dendrogram structures shows a positive correlation with power-law index of 1.73$\pm$0.23, and the leaf L17 hosting W42-MME meets the mass-size conditions for massive star formation. Blue asymmetry is observed in the H$^{12}$CO$^{+}$ (4-3) line profiles of most of the leaves, indicating infall. Overall, our results observationally support the hierarchical and chaotic collapse scenario in the proximity of the MYSO W42-MME.Comment: 16 pages, 13 figures, 1 Table, Accepted for publication in Monthly Notices of the Royal Astronomical Society (MNRAS) Journa

Histological Examination of Collagen and Proteoglycan Changes in Osteoarthritic Menisci

This study sought to examine collagen and proteoglycan changes in the menisci of patients with osteoarthritis (OA). Collagens were examined using picrosirius red, and hematoxylin and eosin. Proteoglycans were examined using safranin-O and alcian blue. Types I and II collagens and aggrecan were examined using immunochemistry. Severe loss of collagens was observed to occur in OA menisci, particularly in the middle and deep zones and collagen networks were less organized than those of normal menisci. In contrast, proteoglycan staining in the middle and deep zones of OA meniscus increased compared to normal control menisci. Immunohistochemistry indicated that types I and II collagens were co-localized and the loss of types I collagen in OA menisci appeared more severe in the middle and deep zones than that in the surface zones. The loss of type II collagen however was severe across all three zones. Immunohistochemistry also indicated elevated aggrecan staining in OA menisci. These findings together indicate that severe loss of collagens and intrameniscal degeneration are hallmarks of OA menisci and that extracellular matrix degeneration occurred in OA menisci follows a pathway different from that occurred in OA articular cartilage. These findings are not only important for a better understanding of the disease process but also important for the development of novel structure-modifying drugs for OA therapy