Stem cells carrier scaffolds for tissue engineering

Mesenchymal Stem Cells (MSCs) can be isolated from several body tissues, including dental tissues. As a result of being capable of differentiating into a variety of cell types, it can be presumed that stem cell therapy has an advantage compared to other tissue repair methods. Objective: The aim of this paper is to provide a review about current and future materials for scaffolds to carry stem cells in tissue engineering in Dentistry, especially for bone tissue repair. Literature review: MSCs have great therapeutic potential in tissue engineering, they can be expanded in vitro , and combined with scaffolds to be inserted into defects to promote healing and tissue replacement. Conclusion: Stem cells from dental tissues have a real potential in Advanced Therapies. The combination of inductive scaffold materials with stem cells might optimize the approaches for bone regeneration. Although there are numerous available biomaterials potentially compatible to combine with MSCs, more studies need to be performed, due to the fact that for each indication there will be a more suitable material according to the defect’s biological and mechanical requirement

Le Fort II osteotomy for medium-face fracture sequel correction

Introduction: Fractures should be treated by a multi-professional team to minimize sequels. The surgery aims to establish a good maxillary, mandibular relationship to improve mastication and phonetics and may benefit esthetics. Objective: to report the surgical procedure with Le Fort II osteotomy for correction of class III dentofacial deformity and the nasomaxillary deficiency caused by trauma. Case report: Patient victim of aggression for 10 years suffered a Le Fort II fracture. The fracture was not treated and the patient developed a severe anteroposterior defect of the nasomaxillary complex andClass III occlusion. The surgery for correcting deformities was performed with the bicoronal, lower eyelid and intraoral accesses. Le Fort II pyramidal osteotomy was performed with a piezoelectric motor to advance 7 mm the nasomaxillary complex. Conclusion: Le Fort II osteotomy is considered satisfactory for advancing the nasomaxillary complex, improving the patient´s psychosocial function and development.Introduction: Fractures should be treated by a multi-professional team to minimize sequels. The surgery aims to establish a good maxillary, mandibular relationship to improve mastication and phonetics and may benefit esthetics. Objective: to report the surgical procedure with Le Fort II osteotomy for correction of class III dentofacial deformity and the nasomaxillary deficiency caused by trauma. Case report: Patient victim of aggression for 10 years suffered a Le Fort II fracture. The fracture was not treated and the patient developed a severe anteroposterior defect of the nasomaxillary complex andClass III occlusion. The surgery for correcting deformities was performed with the bicoronal, lower eyelid and intraoral accesses. Le Fort II pyramidal osteotomy was performed with a piezoelectric motor to advance 7 mm the nasomaxillary complex. Conclusion: Le Fort II osteotomy is considered satisfactory for advancing the nasomaxillary complex, improving the patient´s psychosocial function and development

Effect of the use of different periodontal curettes on the topography and roughness of root surface

Periodontal scaling is the treatment approach most used to remove dental calculus, plaque, and altered cementum from root surface. During root decontamination, the instruments used leave the root rougher and more irregular. Objective: To verify the root surface after mechanical scaling with different Gracey curettes steel through SEM and superficial roughness analyses. Material and methods: Twelve teeth were embedded in acrylic resin. The teeth were instrumented with new Gracey curettes Gracey 5/6 from different brands. The groups (n=2) were divided into: control, no instrumentation (GC); carbon steel (CSN); stainless steel Neumar (SSN); stainless steel Millenium (SSM); premium steel Neumar (PSN); Hu-Friedy (HF). An area measuring 3 x 3 mm2was marked on the distal surface of the root to guide the Reading of the root topography on SEM and rugosimeter. The data were analyzed by a single examiner previously calibrated. SEM analysis was based on scores of the root surface smoothness after scaling. We analyzed the parameters of mean roughness (Ra) and mean roughness deepness (Rz). SEM data were submitted to statistical analysis through Fisher’s exact test (p < 0.002) and roughness data by Anova followed by Student t test. Results: The quality of the active surface of the curette demonstrated by SEM and roughness analyses that it can exert difference in the result regarding to the homogeneity produced after the scaling of root surface. Group SSM demonstrated a homogenous root surface (score 0) in SEM and better smoothness in rugosimeter analysis. Conclusion: According to com the methodology used, the group of curettes that provided better smoothness of root surface after scaling was SSM

Reconstruction of atrophic maxilla with the use of allogenic bone graft and platelet concentrate – clinical case report

Introduction: The use of allogenic bone has the advantage of not producing donor area in the patient, which can decrease the morbidity. However, there is an important vascularization demand for this type of graft, which can be increased with the use of platelet concentrate. Objective: To report a case of atrophic maxilla reconstruction with particle allogenic bone associated with Platelet Rich Fibrin (PRF). Case report: A female patient, who presented a maxilla with a reduced alveolar ridge and pneumatization of the maxillary sinuses, underwent surgery to lift the maxillary sinuses and reconstruct thealveolar ridge, with macro and micro particulate allogenic bone associated with fluid/injectable PRF (i-PRF), and total covering of the graft with PRF membranes, for better accommodation and graft protection. The graft was osseointegrated and able to receive the implants. Conclusion: The technique was effective, presenting several advantages in relation to reconstruction with autologous bone, such as shorter surgical time, amount of material for the graft, and less surgical trauma.Introduction: The use of allogenic bone has the advantage of not producing donor area in the patient, which can decrease the morbidity. However, there is an important vascularization demand for this type of graft, which can be increased with the use of platelet concentrate. Objective: To report a case of atrophic maxilla reconstruction with particle allogenic bone associated with Platelet Rich Fibrin (PRF). Case report: A female patient, who presented a maxilla with a reduced alveolar ridge and pneumatization of the maxillary sinuses, underwent surgery to lift the maxillary sinuses and reconstruct thealveolar ridge, with macro and micro particulate allogenic bone associated with fluid/injectable PRF (i-PRF), and total covering of the graft with PRF membranes, for better accommodation and graft protection. The graft was osseointegrated and able to receive the implants. Conclusion: The technique was effective, presenting several advantages in relation to reconstruction with autologous bone, such as shorter surgical time, amount of material for the graft, and less surgical trauma

Clinical setting influences physiological responses in dental implant patients

is often observed in dental procedures and may cause promote alteration in the physiological responses during implant surgeries. Aim: To evaluate changes in blood pressure and heart rate in patients undergoing dental implant procedures, considering the dental setting as the main variable. Methods: Fifty-five patients who underwent dental implant surgery were evaluated. Thirty-seven were treated at a university clinic and 18 were treated at a private office. Blood pressure and heart rate were measured at the following time-points: at the appointment prior to surgery (T0), immediately before the surgical procedure (T1), during anesthesia (T2), during implant installation (T3), immediately after the surgical procedure (T4) and at the first follow-up appointment after surgery (T5). The data were analyzed by two-way analysis of variance and Students t-test. Results: The university clinic patients had an increase in heart rate at T5 (t53=2.62, p<0.05) compared with private office patients. Systolic blood pressure in university clinic patients was higher at T2 (t53=2.86, p<0.01), T3 (t53=2.64, p<0.05), and T4 (t53=3.15, p<0.01). Diastolic blood pressure at T2 (t53=3.15, p<0.01) and T3 (t53=3.86, p<0.01) were also higher in university clinic patients. Conclusions: These results suggest that the dental setting is a relevant fator when planning dental implant surgery

Clinical setting influences physiological responses in dental implant patients

Abstract Anxiety is often observed in dental procedures and may cause promote alteration in the physiological responses during implant surgeries. Aim: To evaluate changes in blood pressure and heart rate in patients undergoing dental implant procedures, considering the dental setting as the main variable. Methods: Fifty-five patients who underwent dental implant surgery were evaluated. Thirty-seven were treated at a university clinic and 18 were treated at a private office. Blood pressure and heart rate were measured at the following time-points: at the appointment prior to surgery (T0), immediately before the surgical procedure (T1), during anesthesia (T2), during implant installation (T3), immediately after the surgical procedure (T4) and at the first follow-up appointment after surgery (T5). The data were analyzed by two-way analysis of variance and Student&apos;s t-test. Results: The university clinic patients had an increase in heart rate at T5 (t 53 =2.62, p&lt;0.05) compared with private office patients. Systolic blood pressure in university clinic patients was higher at T2 (t 53 =2.86, p&lt;0.01), T3 (t 53 =2.64, p&lt;0.05), and T4 (t 53 =3.15, p&lt;0.01). Diastolic blood pressure at T2 (t 53 =3.15, p&lt;0.01) and T3 (t 53 =3.86, p&lt;0.01) were also higher in university clinic patients. Conclusions: These results suggest that the dental setting is a relevant factor when planning dental implant surgery

Oral and masticatory rehabilitation using osseointegrated dental implants after resective treatment of multicystic ameloblastoma in the lower jaw with a fibula graft

Ameloblastoma is an aggressive odontogenic tumor which typically occurs between third and fourth decade of life that often needs resective approach. Immediate reconstruction may show better results. The treatment of multicystic ameloblastoma in the mandible being a rare case that occurred in the late second decade of life, which was surgically removed along with the affected teeth with safety margins, and the region was immediately reconstructed using a vascularized graft, removed from the fibula. Its integration, in combination with osseointegrated dental implants and fixed implant-supported prostheses, restored chewing function and esthetics. After 6 years from fibular graft and 24 months of dental implants, an excellent outcome was observed, with oral health and normal functions properly restored, and the immediate reconstruction of the mandible in resective cases, associated with oral rehabilitation with dental implants, may be considered a suitable treatment option

Reconstruction of atrophic maxilla with the use of allogenic bone graft and platelet concentrate – clinical case report

Introduction: The use of allogenic bone has the advantage of not producing donor area in the patient, which can decrease the morbidity. However, there is an important vascularization demand for this type of graft, which can be increased with the use of platelet concentrate. Objective: To report a case of atrophic maxilla reconstruction with particle allogenic bone associated with Platelet Rich Fibrin (PRF). Case report: A female patient, who presented a maxilla with a reduced alveolar ridge and pneumatization of the maxillary sinuses, underwent surgery to lift the maxillary sinuses and reconstruct thealveolar ridge, with macro and micro particulate allogenic bone associated with fluid/injectable PRF (i-PRF), and total covering of the graft with PRF membranes, for better accommodation and graft protection. The graft was osseointegrated and able to receive the implants. Conclusion: The technique was effective, presenting several advantages in relation to reconstruction with autologous bone, such as shorter surgical time, amount of material for the graft, and less surgical trauma.Introduction: The use of allogenic bone has the advantage of not producing donor area in the patient, which can decrease the morbidity. However, there is an important vascularization demand for this type of graft, which can be increased with the use of platelet concentrate. Objective: To report a case of atrophic maxilla reconstruction with particle allogenic bone associated with Platelet Rich Fibrin (PRF). Case report: A female patient, who presented a maxilla with a reduced alveolar ridge and pneumatization of the maxillary sinuses, underwent surgery to lift the maxillary sinuses and reconstruct thealveolar ridge, with macro and micro particulate allogenic bone associated with fluid/injectable PRF (i-PRF), and total covering of the graft with PRF membranes, for better accommodation and graft protection. The graft was osseointegrated and able to receive the implants. Conclusion: The technique was effective, presenting several advantages in relation to reconstruction with autologous bone, such as shorter surgical time, amount of material for the graft, and less surgical trauma