90 research outputs found
Hybridized magnonic materials for THz frequency applications
The capability of magnons to hybridize and strongly couple with diverse
excitations offers a promising avenue for realizing and controlling emergent
properties that hold significant potential for applications in devices,
circuits, and information processing. In this letter, we present recent
theoretical and experimental developments in magnon-based hybrid systems,
focusing on the combination of magnon excitation in an antiferromagnet with
other excitations, namely plasmons in a topological insulator, phonons in a 2D
AFM, and photons. The existence of THz frequency magnons, plasmons, and phonons
makes magnon-based hybrid systems particularly appealing for
high-operating-speed devices. In this context, we explore several directions to
advance magnon hybrid systems, including strong coupling between a surface
plasmon and magnon polariton in a TI/AFM bilayer, a giant spin Nernst effect
induced by magnon phonon coupling in 2D AFMs, and control of magnon-photon
coupling using spin torque
MicroRNA miR-378 Regulates Nephronectin Expression Modulating Osteoblast Differentiation by Targeting GalNT-7
MicroRNAs (miRNAs) are small fragments of single-stranded RNA containing 18-24 nucleotides, and are generated from endogenous transcripts. MicroRNAs function in post-transcriptional gene silencing by targeting the 3′-untranslated region (UTR) of mRNAs, resulting in translational repression. We have developed a system to study the role of miRNAs in cell differentiation. We have found that one of the miRNAs tested in our system (miR-378, also called miR-378*) plays a role in modulating nephronectin-mediated differentiation in the osteoblastic cell line, MC3T3-E1. Nephronectin is an extracellular matrix protein, and we have demonstrated that its over-expression enhanced osteoblast differentiation and bone nodule formation. Furthermore, we found that the nephronectin 3′-untranslated region (3′UTR) contains a binding site for miR-378. Stable transfection of MC3T3-E1 cells with miR-378 inhibited cell differentiation. MC3T3-E1 cells stably transfected with nephronectin exhibited higher rates of differentiation and nodule formation as compared with cells transfected with nephronectin containing the 3′UTR in the early stages of development, suggesting that endogenous miR-378 is present and active. However, in the later stages of MC3T3-E1 development, the differentiation rates were opposite, with higher rates of differentiation and nodule formation in the cells over-expressing the 3′UTR of nephronectin. This appeared to be the consequence of competition between nephronectin and UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 7 (GalNAc-T7 or GalNT7) for miR-378 binding, resulting in increased GalNT7 activity, which in turn lead to increased nephronectin glycosylation and product secretion, thereby resulting in a higher rate of osteoblast differentiation
Transcriptome analysis of Sporisorium scitamineum reveals critical environmental signals for fungal sexual mating and filamentous growth
BACKGROUND: Sporisorium scitamineum causes the sugarcane smut disease, one of the most serious constraints to global sugarcane production. S. scitamineum possesses a sexual mating system composed of two mating-type loci, a and b locus. We previously identified and deleted the b locus in S. scitamineum, and found that the resultant SsΔMAT-1b mutant was defective in mating and pathogenicity. RESULTS: To further understand the function of b-mating locus, we carried out transcriptome analysis by comparing the transcripts of the mutant strain SsΔMAT-1b, from which the SsbE1 and SsbW1 homeodomain transcription factors have previously been deleted, with those from the wild-type MAT-1 strain. Also the transcripts from SsΔMAT-1b X MAT-2 were compared with those from wild-type MAT-1 X MAT-2 mating. A total of 209 genes were up-regulated (p < 0.05) in the SsΔMAT-1b mutant, compared to the wild-type MAT-1 strain, while 148 genes down-regulated (p < 0.05). In the mixture, 120 genes were up-regulated (p < 0.05) in SsΔMAT-1b X MAT-2, which failed to mate, compared to the wild-type MAT-1 X MAT-2 mating, and 271 genes down-regulated (p < 0.05). By comparing the up- and down-regulated genes in these two sets, it was found that 15 up-regulated and 37 down-regulated genes were common in non-mating haploid and mating mixture, which indeed could be genes regulated by b-locus. Furthermore, GO and KEGG enrichment analysis suggested that carbon metabolism pathway and stress response mediated by Hog1 MAPK signaling pathway were altered in the non-mating sets. CONCLUSIONS: Experimental validation results indicate that the bE/bW heterodimeric transcriptional factor, encoded by the b-locus, could regulate S. scitamineum sexual mating and/or filamentous growth via modulating glucose metabolism and Hog1-mediating oxidative response. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12864-016-2691-5) contains supplementary material, which is available to authorized users
The Acyl-Homoserine Lactone-Type Quorum-Sensing System Modulates Cell Motility and Virulence of Erwinia chrysanthemi pv. zeae▿
Erwinia chrysanthemi pv. zeae is one of the Erwinia chrysanthemi pathovars that infects on both dicotyledons and monocotyledons. However, little is known about the molecular basis and regulatory mechanisms of its virulence. By using a transposon mutagenesis approach, we cloned the genes coding for an E. chrysanthemi pv. zeae synthase of acyl-homoserine lactone (AHL) quorum-sensing signals (expIEcz) and a cognate response regulator (expREcz). Chromatography analysis showed that expIEcz encoded production of the AHL signal N-(3-oxo-hexanoyl)-homoserine lactone (OHHL). Null mutation of expIEcz in the E. chrysanthemi pv. zeae strain EC1 abolished AHL production, increased bacterial swimming and swarming motility, disabled formation of multicell aggregates, and attenuated virulence of the pathogen on potato tubers. The mutation also marginally reduced the inhibitory activity of E. chrysanthemi pv. zeae on rice seed germination. The mutant phenotypes were rescued by either exogenous addition of AHL signal or in trans expression of expIEcz. These data demonstrate that the AHL-type QS signal plays an essential role in modulation of E. chrysanthemi pv. zeae cell motility and the ability to form multicell aggregates and is involved in regulation of bacterial virulence
Giant spin Nernst effect in a two-dimensional antiferromagnet due to magnetoelastic coupling-induced gaps and interband transitions between magnon-like bands
We analyze theoretically the origin of the spin Nernst and thermal Hall
effects in FePS3 as a realization of two-dimensional antiferromagnet (2D AFM).
We find that a strong magnetoelastic coupling, hybridizing magnetic excitation
(magnon) and elastic excitation (phonon), combined with
time-reversal-symmetry-breaking, results in a Berry curvature hotspots in the
region of anticrossing between the two distinct hybridized bands. Furthermore,
large spin Berry curvature emerges due to interband transitions between two
magnon-like bands, where a small energy gap is induced by magnetoelastic
coupling between such bands that are energetically distant from anticrossing of
hybridized bands. These nonzero Berry curvatures generate topological
transverse transport (i.e., the thermal Hall effect) of hybrid excitations,
dubbed magnon-polaron, as well as of spin (i.e., the spin Nernst effect)
carried by them, in response to applied longitudinal temperature gradient. We
investigate the dependence of the spin Nernst and thermal Hall conductivities
on the applied magnetic field and temperature, unveiling very large spin Nernst
conductivity even at zero magnetic field. Our results suggest FePS3 AFM, which
is already available in 2D form experimentally, as a promising platform to
explore the topological transport of the magnon-polaron quasiparticles at THz
frequencies.Comment: 12 pages, 7 figures; Supplemental Materials is available from
https://mrsec.udel.edu/publications
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