Astroprotective potential of extract from limonium gmelinii for stroke therapy

It's known that proinflammatory cytokines, such asTNF-a, produced by injured neurons and infiltrated leukocytes, involved in every aspect of stroke. Cytokines promote activation of astroglia, induce oxidative stress thereby exacerbating brain tissue damage. There is a number of data indicating that polyphenols can provide protection against neurodegenerative changes. It has been reported previously, that reach with polyphenols extract of Limonium gmelinii exerts a wide range of therapeutic actions. Here, we aimed to study antioxidant and anti-inflammatory potential of L. gmelinii under exposure of TNF-a in vitro

Methodology for the Study of Nationalist Discourse in a Multi-Ethnic Environment

This article reviews the main methodological approaches to the study of the discourse of nationalism in multi-ethnic societies that exist in international research practice.Aim. Purpose is to review the existing approaches to the study of nationalist discourse in modern research, their advantages and disadvantages.Tasks. Objectives are to determine what are the current methods for the study of nationalist discourse in the scientific environment, to examine the limitations of their implementation, to identify the most promising approaches, to specify what are the advantages and disadvantages of the used methodological approaches.Methods. The comparative analysis method was applied to determine the advantages and disadvantages of the methodological approaches to study the discourse of nationalism. The article also used the method of bibliographic data analysis in order to deepen the understanding of the existing methodological approaches to the study of the discourse of nationalism by searching and studying scientific materials and works of scientists.Results. The article deals with the quantitative and qualitative methods to study the nationalist discourse in multi-ethnic environment, the difficulties and limitations of the implementation of these or those methods, as well as their advantages and disadvantages. Special attention was paid to the method of content analysis of social networks and blogosphere, since today the study of sentiments in social networks is gaining importance, due to the increasing breadth of audience coverage in different countries as well as the emergence of new formats of citizen participation, such as online participation.Conclusions. Today, the discourse related to nationalist sentiments is most prevalent in internet, for a number of reasons, including the speed and ease of presenting information and the horizontal connectivity. Therefore, in multi-ethnic societies, great importance should be given to scrutinising content on social media including the use of ‘hate speech’. In this regard, the paper makes recommendations for analysing the information space and strengthening work in this direction

Numerical solution of a problem on bending oscillation of a rod

In article considered the problem of curved rod fluctuation with Jungs module. Shown the civility of the problem formulation. For solution used integro - interpolation method. Constructed implicit differential scheme, which realized by five - point sweep method. Conducted numerical calculations showed coincidence of theoretical calculation values of solution. Calculations conducted on the system of computer algebra Wolfram Mathematica. Results of calculations are given for two cases of fixing ends of the rod: both ends are fixed and one end is fixed other is free

A New Prenylated Isoflavonoid from Limonium leptophyllum

© 2019, Mary Ann Liebert, Inc., publishers 2019. Purpose: Cannabidiol (CBD), active component of plant Cannabis sativa, has anti-inflammatory properties that could potentially help treat diabetic retinopathy-induced pain and inflammation. However, CBD is a lipophilic molecule making its topical delivery to back of the eye challenging. This study aims at improving ocular penetration of CBD by means of analog derivatization. Methods: Analogs were designed using various ligands, such as amino acids (AAs) and dicarboxylic acids (DCAs) and their combinations. Select analogs were screened in vitro with respect to their stability in ocular tissue homogenates. Based on in vitro stability, analogs were selected for in rabbits testing. Formulations containing these compounds were tested in rabbits to determine ocular tissue disposition of CBD and the analogs after topical application. The rabbits were sacrificed 90 min post-topical application and the aqueous humor, vitreous humor (VH), iris-ciliary bodies (IC), and retina-choroid (RC) were analyzed for CBD and analog content. Results: CBD-divalinate-dihemisuccinate (CBD-Di-VHS) and CBD-divalinate (CBD-Di-Val) were stable in the ocular tissue homogenates. Post-topical application, CBD and CBD-Di-Val analog levels were detected only in RC. Dosing of CBD-Di-VHS nanoemulsion generated analog levels both in the VH and in the RC, respectively. In contrast, post dosing of CBD-monovalinate-monohemisuccinate (CBD-Mono-VHS), both the analog and CBD were detected in the IC and RC. Conclusion: The analogs demonstrated superior penetration into ocular tissues in comparison with CBD. CBD-Di-VHS and CBD-Mono-VHS exhibited better permeation properties, possibly due to improved stability and physicochemical characteristics imparted by AA and DCA combination derivatives

Developing Creative Potential of Future Teachers: Research and Results

AbstractThe total globalization and integration of national education into global educational space requires a higher quality of vocational training of future teachers whose competence and creative activeness significantly affects the level of intellectual potential of society. The relevance of this issue becomes more important given the tendency of decrease in students’ motivation to learn, to acquire basic academic school knowledge. This makes it necessary to find innovational approaches in educating of future teachers during the process of vocational training.One of these approaches employs a purposeful development of creative potential among future teachers through introduction of innovational interactive educative methods into the training process which would encourage maximal development of their self-actualization, self-realization and self-improvement. This article gives consideration to the main results in experimental work on purposeful maturation of preparedness of future teachers to creative pedagogical work in university

Study of the Chemical Composition of <i>Rosa beggeriana</i> Schrenk’s Fruits and Leaves

Rosa species are widely used in folk medicine in different countries of Asia and Europe, but not all species are studied in-depth. For instance, Rosa beggeriana Schrenk, a plant which grows in Central Asia, Iran, and some parts of China, is little described in articles. Column and thin-layer chromatography methods were used to isolate biologically active substances. From a study of fruits and leaves of Rosa beggeriana Schrenk, a large number of compounds were identified, seven of which were isolated: 3β,23-dihydroxyurs-12-ene (1), β-sitosterol (2), betulin (3), (+)-catechin (4), lupeol (5), ethyl linoleate (6), and ethyl linolenoate (7). Their structures were elucidated by 1H, DEPT and 13C NMR spectroscopy, mass spectrometry, and GC-MS (gas chromatography–mass spectrometry). The study also identified the structures of organic compounds, including volatile esters and acids. Consequently, comprehensive data were acquired concerning the chemical constitution of said botanical specimen

Adiponectin plasma levels in patients with age-related dementia

Adiponectin play a significant role in the regulation of type 2 diabetes, obesity, atherosclerosis, non-alcoholic fatty liver disease and neurodegenerative disorders. Evidence suggests that adiponectin may be an independent risk factor for all-cause dementia and Alzheimer's disease (AD) and the level of adiponectin in plasma reflects its level in cerebrospinal fluid (CSF). Studies demonstrated that elevated adiponectin level in blood was associated with an increased risk of dementia and AD in women and indicates that the sex dimorphism with regard to adiponectin levels association with Alzheimer's disease was clearly observe

Phytochemical Profiles and In Vitro Immunomodulatory Activities of Extracts Obtained from <i>Limonium gmelinii</i> Using Different Extraction Methods

Limonium (L.) gmelinii is a valuable pharmacopoeial Kazakhstani plant. Several studies have reported on the various biological activities of the plant. The purpose of our research was to study and compare the extraction yields, immunomodulatory activities, and chemical compositions of extracts from the above-ground parts of L. gmelinii obtained via conventional extraction (CE; Extract 1) and ultrasound-assisted extraction (UAE; Extract 2). The extracts were characterized by a considerable number of polyphenols and flavonoids: 378.1 ± 4.5 and 382.2 ± 3.3 GAE mg/g, and 90.22 ± 2.8 and 94.61 ± 1.9 QE mg/g in Extract 1 and Extract 2, respectively. Extract 2 had a slightly higher extraction yield (33.5 ± 2.4%) than Extract 1 (30.2 ± 1.6%). Liquid Chromatography–Diode-Array Detection–Electrospray Ionization–Quadrupole Time-of-Flight Mass Spectrometry (LC-QToF-MS) revealed the presence of 54 biologically active compounds in both extracts. It was shown that the studied extracts stimulate the secretion of TNF-α and IL-6 by intact mouse peritoneal macrophages and splenic lymphocytes, whilst they have an inhibitory effect on the secretion of these cytokines by activated immune cells. Both extracts demonstrated similar patterns of stimulation and inhibition in a splenocyte proliferation assay. Altogether, the L. gmelinii extracts obtained via CE and UAE might be suggested as effective immunomodulatory agents. The application of UAE for this purpose seems to be more efficient with a view of obtaining of a highly potent extract in a much shorter time