No requirement of TRPV1 in long-term potentiation or long-term depression in the anterior cingulate cortex

One major interest in the study of transient receptor potential vanilloid type 1 (TRPV1) in sensory system is that it may serve as a drug target for treating chronic pain. While the roles of TRPV1 in peripheral nociception and sensitization have been well documented, less is known about its contribution to pain-related cortical plasticity. Here, we used 64 multi-electrode array recording to examine the potential role of TRPV1 in two major forms of synaptic plasticity, long-term potentiation (LTP) and long-term depression (LTD), in the anterior cingulate cortex (ACC). We found that pharmacological blockade of TRPV1 with either [(E)-3-(4-t-Butylphenyl)-N-(2,3-dihydrobenzo[b][1,4]dioxin-6-yl)acrylamide] (AMG9810, 10 μM) or N-(3-methoxyphenyl)-4-chlorocinnamide (SB366791, 20 μM) failed to affect LTP induced by strong theta burst stimulation in the ACC of adult mice. Similarly, neither AMG9810 nor SB366791 blocked the cingulate LTD induced by low-frequency stimulation. Analysis of the results from different layers of the ACC obtained the same conclusions. Spatial distribution of LTP or LTD-showing channels among the ACC network was also unaltered by the TRPV1 antagonists. Since cortical LTP and LTD in the ACC play critical roles in chronic pain triggered by inflammation or nerve injury, our findings suggest that TRPV1 may not be a viable target for treating chronic pain, especially at the cortical level

EdgeYOLO: An Edge-Real-Time Object Detector

This paper proposes an efficient, low-complexity and anchor-free object detector based on the state-of-the-art YOLO framework, which can be implemented in real time on edge computing platforms. We develop an enhanced data augmentation method to effectively suppress overfitting during training, and design a hybrid random loss function to improve the detection accuracy of small objects. Inspired by FCOS, a lighter and more efficient decoupled head is proposed, and its inference speed can be improved with little loss of precision. Our baseline model can reach the accuracy of 50.6% AP50:95 and 69.8% AP50 in MS COCO2017 dataset, 26.4% AP50:95 and 44.8% AP50 in VisDrone2019-DET dataset, and it meets real-time requirements (FPS>=30) on edge-computing device Nvidia Jetson AGX Xavier. We also designed lighter models with less parameters for edge computing devices with lower computing power, which also show better performances. Our source code, hyper-parameters and model weights are all available at https://github.com/LSH9832/edgeyolo

4,4′,5,5′-Tetra­phenyl-3,3′-[methyl­idene­bis(sulfanedi­yl)]bis­(4H-1,2,4-triazole)

The asymmetric unit of the title compound, C29H22N6S2, contains one half-mol­ecule situated on a twofold rotational axis. The two triazole rings form a dihedral angle of 27.6 (2)°. In the crystal, weak inter­molecular C—H⋯N hydrogen bonds link the mol­ecules into ribbons extending along [001]

The Microsoft System for VoxCeleb Speaker Recognition Challenge 2022

In this report, we describe our submitted system for track 2 of the VoxCeleb Speaker Recognition Challenge 2022 (VoxSRC-22). We fuse a variety of good-performing models ranging from supervised models to self-supervised learning(SSL) pre-trained models. The supervised models, trained using VoxCeleb-2 dev data, consist of ECAPA-TDNN and Res2Net in a very deep structure. The SSL pre-trained models, wav2vec and wavLM, are trained using large scale unlabeled speech data up to million hours. These models are cascaded with ECAPA-TDNN and further fine-tuned in a supervised fashion to extract the speaker representations. All 13 models are applied with score normalization and calibration and then fused into the the submitted system. We also explore the audio quality measures in the calibration stage such as duration, SNR, T60, and MOS. The best submitted system achieves 0.073 in minDCF and 1.436% in EER on the VoxSRC-22 evaluation set.Comment: 3 pages, 3 tables, VoxSRC202

FBXW8-Dependent Degradation of MRFAP1 in Anaphase Controls Mitotic Cell Death

Mof4 family associated protein 1 (MRFAP1) is a 14 kDa nuclear protein, which involves in maintaining normal histone modification levels by negatively regulating recruitment of the NuA4 (nucleosome acetyltransferase of H4) histone acetyltransferase complex to chromatin. MRFAP1 has been identified as one of the most up-regulated proteins after NEDD8 (neural precursor cell expressed developmentally down-regulated 8) inhibition in multiple human cell lines. However, the biological function of MRFAP1 and the E3 ligase that targets MRFAP1 for destruction remain mysterious. Here we show, by using an immunoprecipitation-based proteomics screen, that MRFAP1 is an interactor of the F-box protein FBXW8. MRFAP1 is degraded by means of the ubiquitin ligase Cul7/FBXW8 during mitotic anaphase-telophase transition and accumulated in mitotic metaphase. Overexpression of FBXW8 increased the polyubiquitination and decreased the stability of MRFAP1, whereas knockdown of FBXW8 prolonged the half-life of MRFAP1. Moreover, forced expression of MRFAP1 in HeLa cells caused growth retardation and genomic instability, leading to severe mitotic cell death. Thus, Cul7/FBXW8-mediated destruction of MRFAP1 is a regulatory component monitoring the anaphase-telophase transition and preventing genomic instability

Overview of research progress on the association of dietary potassium intake with serum potassium and survival in hemodialysis patients, does dietary potassium restriction really benefit hemodialysis patients?

For the general population, increasing potassium intake can reduce the incidence of cardiovascular and cerebrovascular diseases. However, since hyperkalemia is a common and life-threatening complication in maintenance hemodialysis patients, which can increase the risk of malignant arrhythmia and sudden death, the current mainstream of management for hemodialysis patients is dietary potassium restriction in order to prevent hyperkalemia. Hemodialysis patients are usually advised to reduce dietary potassium intake and limit potassium-rich fruits and vegetables, but there is limited evidence to support this approach can reduce mortality and improve quality of life. There is still no consistent conclusion on the association between dietary potassium intake and serum potassium and survival in hemodialysis patients. According to the current small observational studies, there was little or even no association between dietary potassium intake and serum potassium in hemodialysis patients when assurance of adequate dialysis and specific dietary patterns (such as the plant-based diet mentioned in the article) are being followed, and excessive dietary potassium restriction may not benefit the survival of hemodialysis patients. Additionally, when assessing the effect of diet on serum potassium, researchers should not only focus on the potassium content of foods, but also consider the type of food and the content of other nutrients. However, more large-scale, multi-center clinical trials are required to provide high-quality evidence support. Besides, further research is also needed to determine the optimal daily potassium intake and beneficial dietary patterns for hemodialysis patients

The origin of ultrahigh piezoelectricity in relaxor-ferroelectric solid solution crystals

The discovery of ultrahigh piezoelectricity in relaxor-ferroelectric solid solution single crystals is a breakthrough in ferroelectric materials. A key signature of relaxor-ferroelectric solid solutions is the existence of polar nanoregions, a nanoscale inhomogeneity, that coexist with normal ferroelectric domains. Despite two decades of extensive studies, the contribution of polar nanoregions to the underlying piezoelectric properties of relaxor ferroelectrics has yet to be established. Here we quantitatively characterize the contribution of polar nanoregions to the dielectric/piezoelectric responses of relaxor-ferroelectric crystals using a combination of cryogenic experiments and phase-field simulations. The contribution of polar nanoregions to the room-temperature dielectric and piezoelectric properties is in the range of 50-80%. A mesoscale mechanism is proposed to reveal the origin of the high piezoelectricity in relaxor ferroelectrics, where the polar nanoregions aligned in a ferroelectric matrix can facilitate polarization rotation. This mechanism emphasizes the critical role of local structure on the macroscopic properties of ferroelectric materials