239 research outputs found
Assessment of Express Delivery Service Based on SERVQUAL Model
With the rapid development of electronic commerce, the public demand for express delivery is increasing, however, there are still lack of service quality and high service complaint ratein the process of rapid development. Based on the SERVQUAL model, this paper analyzes the current situation of the whole express service industry quality, From five aspects: tangibles, reliability, responsiveness, assurance, empathy , constructs the express service quality model based on the SERVQUAL model, and use the integration of AHP and fuzzy comprehensive evaluation to evaluate the model construction. Finally, this paper takes the express company as an example to make an empirical study, and puts forward the corresponding improvement strategy.The research has an important reference value for the improvement of the service quality of the major express delivery companies
A 2-Cys Peroxiredoxin in Response to Oxidative Stress in the Pine Wood Nematode, \u3cem\u3eBursaphelenchus xylophilus\u3c/em\u3e
The pine wood nematode, Bursaphelenchus xylophilus, is the causal agent of pine wilt disease that has devastated pine forests in Asia. Parasitic nematodes are known to have evolved antioxidant stress responses that defend against host plant defenses. In this study, the infestation of whitebark pine, Pinus bungean, with B. xylophilus led to a significant increase in plant hydrogen peroxide (H2O2) and salicylic acid levels. Correspondingly, the expression of an antioxidative enzyme, 2-Cysteine peroxiredoxin (BxPrx), was elevated in B. xylophilus following the H2O2 treatments. Recombinant BxPrx, a thermal stabile and pH tolerant enzyme, exhibited high level of antioxidant activity against H2O2, suggesting that it is capable of protecting cells from free radical attacks. Immunohistochemical localization study showed that BxPrx was broadly expressed across different tissues and could be secreted outside the nematode. Finally, the number of BxPrx homologs in both dauer-like and fungi-feeding B. xylophilus were comparable based on bioinformatics analysis of existing EST libraries, indicating a potential role of BxPrx in both propagative and dispersal nematodes. These combined results suggest that BxPrx is a key genetic factor facilitating the infestation and distribution of B. xylophilus within pine hosts, and consequently the spread of pine wilt disease
Mutational analysis of Brh2 reveals requirements for compensating mediator functions
This is the peer-reviewed version of the article: Kojic, M., Zhou, Q., Fan, J., & Holloman, W. K. (2011). Mutational analysis of Brh2 reveals requirements for compensating mediator functions. Molecular Microbiology, 79(1), 180–191.[ https://doi.org/10.1111/j.1365-2958.2010.07440.x]Published version: [https://imagine.imgge.bg.ac.rs/handle/123456789/476
Reference Gene Selection for Transcriptional Profiling in \u3cem\u3eCryptocercus punctulatus\u3c/em\u3e, an Evolutionary Link between Isoptera and Blattodea
The subsocial life style and wood-feeding capability of Cryptocercus gives us an evolutionary key to unlock some outstanding questions in biology. With the advent of the Genomics Era, there is an unprecedented opportunity to address the evolution of eusociality and the acquisition of lignocellulases at the genetic level. However, to quantify gene expression, an appropriate normalization strategy is warranted to control for the non-specific variations among samples across different experimental conditions. To search for the internal references, 10 housekeeping genes from a gut transcriptome of a wood-feeding cockroach, Cryptocercus punctulatus, were selected as the candidates for the RT-qPCR analysis. The expression profiles of these candidates, including ACT, EF1α, GAPDH, HSP60, HSP70, αTUB, UBC, RPS18, ATPase and GST, were analyzed using a panel of analytical tools, including geNorm, NormFinder, BestKeeper, and comparative ΔCT method. RefFinder, a comprehensive ranking system integrating all four above-mentioned algorithms, rated ACT as the most stable reference gene for different developmental stages and tissues. Expression analysis of the target genes, Hex-1 and Cell-1, using the most or the least appropriate reference genes and a single or multiple normalizers signified this research. Our finding is the first step toward establishing a standardized RT-qPCR analysis in Cryptocercus
High-Efficiency Resonant Beam Charging and Communication
With the development of Internet of Things (IoT), demands of power and data
for IoT devices increase drastically. In order to resolve the supply-demand
contradiction, simultaneous wireless information and power transfer (SWIPT) has
been envisioned as an enabling technology by providing high-power energy
transfer and high-rate data delivering concurrently. In this paper, we
introduce a high-efficiency resonant beam (RB) charging and communication
scheme. The scheme utilizes the semiconductor materials as gain medium, which
has a better energy absorption capacity compared with the traditional
solid-state one. Moreover, to match the gain size and reduce the transmission
loss, the telescope internal modulator (TIM) are adopted in the scheme, which
can concentrate beams.To evaluate the scheme SWIPT performance, we establish an
analytical model and study the influence factors of its beam transmission,
energy conversion, output power, and spectral efficiency. Numerical results
shows that the proposed RB system can realize 16 W electric power output with
11 % end-to-end conversion efficiency, and support 18 bit/s/Hz spectral
efficiency for communication
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