559 research outputs found
A comparison of the Medieval Warm Period, Little Ice Age and 20th century warming simulated by the FGOALS climate system model
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The effect of horizontal resolution on the representation of the global monsoon annual cycle in Atmospheric General Circulation Models
The sensitivity of the representation of the global monsoon annual cycle to horizontal resolution is compared in three Atmospheric General Circulation Models (AGCMs): the Met Office Unified Model-Global Atmosphere 3.0 (MetUM-GA3), the Meteorological Research Institute AGCM3 (MRI-AGCM3) and Global High Resolution AGCM from the Geophysical Fluid Dynamics Laboratory (GFDL-HiRAM). For each model, we use two horizontal resolution configurations for the period 1998â2008. Increasing resolution consistently improves simulated precipitation and low-level circulation of the annual mean and the first two annual cycle modes, as measured by pattern correlation coefficient and Equitable Threat Score. Improvements in simulating the summer monsoon onset and withdrawal are region-dependent. No consistent response to resolution is found in simulating summer monsoon retreat. Regionally, increased resolution reduces the positive bias in simulated annual mean precipitation, the two annual-cycle modes over the West African monsoon and Northwestern Pacific monsoon. An overestimation of the solstitial mode and an underestimation of the equinoctial asymmetric mode of the East Asian monsoon are reduced in all high-resolution configurations. Systematic errors exist in lower-resolution models for simulating the onset and withdrawal of the summer monsoon. Higher resolution models consistently improve the early summer monsoon onset over East Asia and West Africa, but substantial differences exist in the responses over Indian monsoon region, where biases differ across the three low-resolution AGCMs. This study demonstrates the importance of a multi-model comparison when examining the added value of resolution and the importance of model physical parameterizations for the Indian monsoon simulation
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Added value of high resolution models in simulating global precipitation characteristics
Climate models tend to overestimate percentage of the contribution (to total precipitation) and frequency of light rainfall while underestimate the heavy rainfall. This article investigates the added value of high resolution of atmospheric general circulation models (AGCMs) in simulating the characteristics of global precipitation, in particular extremes. Three AGCMs, global high resolution atmospheric model from the Geophysical Fluid Dynamics Laboratory (GFDL-HiRAM), the Meteorological Research Institute-atmospheric general circulation model (MRI-AGCM) and the Met Office Unified Model (MetUM), each with one high and one low resolution configurations for the period 1998â2008 are used in this study. Some consistent improvements are found across all three AGCMs with increasing model resolution from 50â83 to 20â35 km. A reduction in global mean frequency and amount percentile of light rainfall (20 mm dayâ1) are shown in high resolution models of GFDL-HiRAM and MRI-AGCM, while the improvement in MetUM is not obvious. A consistent response to high resolution across the three AGCMs is seen from the increase of light rainfall frequency and amount percentile over the desert regions, particularly over the ocean desert regions. It suppresses the overestimation of CDD over ocean desert regions and makes a better performance in high resolution models of GFDL-HiRAM and MRI-AGCM, but worse in MetUM-N512. The impact of model resolution differs greatly among the three AGCMs in simulating the fraction of total precipitation exceeding the 95th percentile daily wet day precipitation. Inconsistencies among models with increased resolution mainly appear over the tropical oceans and in simulating extreme wet conditions, probably due to different reactions of dynamical and physical processes to the resolution, indicating their crucial role in high resolution modelling
Phytochrome B Negatively Affects Cold Tolerance by Regulating OsDREB1 Gene Expression through Phytochrome Interacting Factor-Like Protein OsPIL16 in Rice
Overexpression of Pear (Pyrus pyrifolia) CAD2 in Tomato Affects Lignin Content
PpCAD2 was originally isolated from the âWangkumbaeâ pear (Pyrus pyrifolia Nakai), and it encodes for cinnamyl alcohol dehydrogenase (CAD), which is a key enzyme in the lignin biosynthesis pathway. In order to verify the function of PpCAD2, transgenic tomato (Solanum lycopersicum) âMicro-Tomâ plants were generated using over-expression constructs via the agrobacterium-mediated transformation method. The results showed that the PpCAD2 over-expression transgenic tomato plant had a strong growth vigor. Furthermore, these PpCAD2 over-expression transgenic tomato plants contained a higher lignin content and CAD enzymatic activity in the stem, leaf and fruit pericarp tissues, and formed a greater number of vessel elements in the stem and leaf vein, compared to wild type tomato plants. This study clearly indicated that overexpressing PpCAD2 increased the lignin deposition of transgenic tomato plants, and thus validated the function of PpCAD2 in lignin biosynthesis
Sequential Star Formation in the filamentary structures of Planck Galactic cold clump G181.84+0.31
We present a multi-wavelength study of the Planck cold clump G181.84+0.31,
which is located at the northern end of the extended filamentary structure
S242. We have extracted 9 compact dense cores from the SCUBA-2 850 um map, and
we have identified 18 young stellar objects (YSOs, 4 Class I and 14 Class II)
based on their Spitzer, Wide-field Infrared Survey Explorer (WISE) and
Two-Micron All-Sky Survey (2MASS) near- and mid-infrared colours. The dense
cores and YSOs are mainly distributed along the filamentary structures of
G181.84 and are well traced by HCO(1-0) and NH(1-0)
spectral-line emission. We find signatures of sequential star formation
activities in G181.84: dense cores and YSOs located in the northern and
southern sub-structures are younger than those in the central region. We also
detect global velocity gradients of about 0.80.05 km spc and
1.00.05 km spc along the northern and southern
sub-structures, respectively, and local velocity gradients of 1.20.1 km
spc in the central substructure. These results may be due to the
fact that the global collapse of the extended filamentary structure S242 is
driven by an edge effect, for which the filament edges collapse first and then
further trigger star formation activities inward. We identify three
substructures in G181.84 and estimate their critical masses per unit length,
which are 10115 M pc, 568 M
pc and 284 M pc, respectively. These values are
all lower than the observed values ( 200 M pc),
suggesting that these sub-structures are gravitationally unstable.Comment: 20 pages, 17 figures, article, accepte
Hydrocode modeling of the spallation process during hypervelocity impacts: Implications for the ejection of Martian meteorites
Hypervelocity ejection of material by impact spallation is considered a
plausible mechanism for material exchange between two planetary bodies. We have
modeled the spallation process during vertical impacts over a range of impact
velocities from 6 to 21 km/s using both grid- and particle-based hydrocode
models. The Tillotson equations of state, which are able to treat the nonlinear
dependence of density on pressure and thermal pressure in the strongly shocked
matter, were used to study the hydrodynamic and thermodynamic response after
impacts. The effects of material strength and gravitational acceleration were
not considered. A two-dimensional time-dependent pressure field within a
1.5-fold projectile radius from the impact point was investigated in
cylindrical coordinates to address the generation of spalled material. A
resolution test was also performed to reject ejected materials with peak
pressures that were too low due to artificial viscosity. The relationship
between ejection velocity veject and peak pressure Ppeak was also derived. Our
approach shows that late stage acceleration in an ejecta curtain occurs due to
the compressible nature of the ejecta, resulting in an ejection velocity that
can be higher than the ideal maximum of the resultant particle velocity after
passage of a shock wave. We also calculate the ejecta mass that can escape from
a planet like Mars (i.e., veject higher than 5 km/s) that matches the
petrographic constraints from Martian meteorites, and which occurs when Ppeak
from 30-50 GPa. Although the mass of such ejecta is limited to from 0.1-1
percent of the projectile mass in vertical impacts, this is sufficient for
spallation to have been a plausible mechanism for the ejection of Martian
meteorites. Finally, we propose that impact spallation is a plausible mechanism
for the generation of tektites.Comment: 67 pages, 28 figures, accepted for publication in Icaru
Cloning of a gene encoding glycosyltransferase from Pueraria lobata (Wild.) Ohwi and its expression in Pichia pastoris
The key enzyme of puerarin biosynthesis in Pueraria lobata (Willd.) Ohwi was unclear but may involve glycosylation. To investigate the regulation of puerarin biosynthesis, a putative UDP-dependent glycosyltransferase (UGT) gene, PlUGT1 was isolated from P. lobata root, which contained abundant puerarin. PlUGT1 encoded 480 deduced amino acid residues with a conserved UDP-glucose-binding domain, which has 61 to 84% similarity to homologues from other plant species. SDS polyacrylamide gel electrophoresis and western blotting results showed that, fusion protein migrated as a single protein band with a molecular weight of 55 kDa. A yeast expression vector pPICZA-PlUGT1 was constructed and was transformed into Pichia pastoris strain GS115. Several recombinants containing multi-copy expression cassettes were obtained on the zeocin-YPD plate and confirmed by southern dot blotting. The yield of PlUGT1 attained 0.05 g/l when recombinant cells were cultured at pH 5.5, 30°C and induced with 0.5% methanol for 72 h. The expression of PlUGT1 protein correlates positively with the copy numbers of PlUGT1 in transformed yeast cells. These results suggest that, the PlUGT1 protein can be expressed efficiently in the P. pastoris expression system and may supply a new economic and convenient way for the production of PlUGT1 protein.Keywords: Pueraria lobata (Willd.) Ohwi, glycosyltransferase, cloning, expression, Pichia pastori
Molecular Cloning and Characterization of a P-Glycoprotein from the Diamondback Moth, \u3cem\u3ePlutella xylostella\u3c/em\u3e (Lepidoptera: Plutellidae)
Macrocyclic lactones such as abamectin and ivermectin constitute an important class of broad-spectrum insecticides. Widespread resistance to synthetic insecticides, including abamectin and ivermectin, poses a serious threat to the management of diamondback moth, Plutella xylostella (L.) (Lepidoptera: Plutellidae), a major pest of cruciferous plants worldwide. P-glycoprotein (Pgp), a member of the ABC transporter superfamily, plays a crucial role in the removal of amphiphilic xenobiotics, suggesting a mechanism for drug resistance in target organisms. In this study, PxPgp1, a putative Pgp gene from P. xylostella, was cloned and characterized. The open reading frame (ORF) of PxPgp1 consists of 3774 nucleotides, which encodes a 1257-amino acid peptide. The deduced PxPgp1 protein possesses structural characteristics of a typical Pgp, and clusters within the insect ABCB1. PxPgp1 was expressed throughout all developmental stages, and showed the highest expression level in adult males. PxPgp1 was highly expressed in midgut, malpighian tubules and testes. Elevated expression of PxPgp1 was observed in P. xylostella strains after they were exposed to the abamectin treatment. In addition, the constitutive expressions of PxPgp1 were significantly higher in laboratory-selected and field-collected resistant strains in comparison to their susceptible counterpart
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