On Environmental Protection and Sustainable Utilization of Land Resources

In today’s era, with the rapid development of social economy and the rapid increase of urban population, the demand for land resources is becoming increasingly strong. However, as is well known, the land resources on Earth are limited. If we want to achieve long-term development, we must take the path of sustainable development and use land resources sustainably. At the same time, the sustainable use of land resources and environmental protection are also inseparable, and the two are complementary. At present, China should rely on the superiority of the socialist system, mobilize various forces, coordinate planning, highlight key points, and implement step by step, while following natural and economic laws, to achieve the goals of environmental improvement and sustainable land resource utilization as soon as possible

Main Technical Analysis of Sewage Treatment in Environmental Engineering

In recent years, China has developed rapidly and is gradually moving towards a moderately prosperous society in all respects. In the context of this era, increasingly validated environmental issues have always plagued China’s economic development. In the early stages of reform and opening up, while the economy developed rapidly, China’s attention to environmental protection was not high, resulting in increasing pressure on environmental protection. The sewage generated by most urban life directly affects the development of the urban environment. Urban sewage treatment can recycle water resources, which has a good effect on protecting the social and ecological environment. This paper analyzes the common problems of sewage treatment in Environmental engineering, and expounds the common technologies of sewage treatment in China at present for your reference

A New Algorithm for Bearings-Only Parametric Trajectory Tracking

AbstractFor the single sensor target tracking with bearings-only measurements, a novel trajectory invariable-information target tracking algorithm was proposed, and bearings-only target can be tracked by the parameter trajectory. For the measure frequency of sensor is high, the mathematic model of bearings-only tracking is analyzed by dividing the trajectory into many linearization parts. The tracking parameter of bearings-only target trajectory is deduced, so the bearings-only target can be tracked by the parameter trajectory. The simulation results show that the new algorithm has a favorable tracking precision

Upregulation of Mitochondrial Uncoupling Protein-2 by the AMP-Activated Protein Kinase in Endothelial Cells Attenuates Oxidative Stress in Diabetes

OBJECTIVE—Recent evidence suggests that the AMP-activated protein kinase (AMPK) is an important therapeutic target for diabetes. The present study was conducted to determine how AMPK activation suppressed tyrosine nitration of prostacyclin synthase in diabetes

Numerical analysis of electro-convection in dielectric liquids with residual conductivity

Injection-induced electro-convection (EC) of dielectric liquids is a fundamental problem in electrohydrodynamics. However, most previous studies with this type of EC assume that the liquid is perfectly insulating. By perfectly insulating, we mean an ideal liquid with zero conductivity, and in this situation, the free charges in the bulk liquid originate entirely from the injection of ions. In this study, we perform a numerical analysis with the EC of dielectric liquids with a certain residual conductivity based on a dissociation–injection model. The spatiotemporal distributions of the flow field, electric field, and positive/negative charge density in the parallel plate configuration are solved utilizing the finite volume method. It is found that the residual conductivity inhibits the onset of EC flow, as well as the strength of the flow field. The flow features and bifurcations are studied in various scenarios with three different injection strengths in the strong, medium, and weak regimes. Three distinct bifurcation sequences with abundant features are observed by continually increasing or decreasing the electric Reynolds number. The present study shows that the residual conductivity significantly affects the bifurcation process and the corresponding critical point of EC flows.Ministerio de Ciencia, Innovación y Universidades PGC2018-099217-B-I0

Regularly log-periodic functions and some applications

We prove a Tauberian theorem for the Laplace--Stieltjes transform and Karamata-type theorems in the framework of regularly log-periodic functions. As an application we determine the exact tail behavior of fixed points of certain type smoothing transforms

Inhibitory Effect of Cinobufagin on L-Type C a

Cinobufagin (CBG), a major bioactive ingredient of the bufanolide steroid compounds of Chan Su, has been widely used to treat coronary heart disease. At present, the effect of CBG on the L-type Ca2+ current (ICa-L) of ventricular myocytes remains undefined. The aim of the present study was to characterize the effect of CBG on intracellular Ca2+ ([Ca2+]i) handling and cell contractility in rat ventricular myocytes. CBG was investigated by determining its influence on ICa-L, Ca2+ transient, and contractility in rat ventricular myocytes using the whole-cell patch-clamp technique and video-based edge-detection and dual-excitation fluorescence photomultiplier systems. The dose of CBG (10−8 M) decreased the maximal inhibition of CBG by 47.93%. CBG reduced ICa-L in a concentration-dependent manner with an IC50 of 4 × 10−10 M, upshifted the current-voltage curve of ICa-L, and shifted the activation and inactivation curves of ICa-L leftward. Moreover, CBG diminished the amplitude of the cell shortening and Ca2+ transients with a decrease in the time to peak (Tp) and the time to 50% of the baseline (Tr). CBG inhibited L-type Ca2+ channels, and reduced [Ca2+]i and contractility in adult rat ventricular myocytes. These findings contribute to the understanding of the cardioprotective efficacy of CBG

Stratification of HPV-induced cervical pathology using the virally encoded molecular marker E4 in combination with p16 or MCM.

High-risk human papillomavirus (HPV) types cause cervical lesions of varying severity, ranging from transient productive infections to high-grade neoplasia. Disease stratification requires the examination of lesional pathology, and possibly also the detection of biomarkers. P16(INK4a) and MCM are established surrogates of high-risk HPV E6/E7 activity, and can be extensively expressed in high-grade lesions. Here we have combined these two cellular biomarkers with detection of the abundant HPV-encoded E4 protein in order to identify both productive and transforming lesions. This approach has allowed us to distinguish true papillomavirus infections from similar pathologies, and has allowed us to divide the heterogeneous CIN2 category into those that are CIN1-like and express E4, and those that more closely resemble nonproductive CIN3. To achieve this, 530 lesional areas were evaluated according to standard pathology criteria and by using a multiple staining approach that allows us to superimpose biomarker patterns either singly or in combination onto an annotated hematoxylin and eosin (H&E) image. Conventional grading of neoplasia was established by review panel, and compared directly with the composite molecular pathology visualized on the same tissue section. The detection of E4 coincided with the onset of vacuolation, becoming abundant in koilocytes as the MCM marker declined and cells lost their defined nuclear margins as visualized by standard H&E staining. Of the dual marker approaches, p16(INK4a) and E4 appeared most promising, with E4 generally identifying areas of low-grade disease even when p16(INK4a) was present. Extensive p16(INK4a) expression usually coincided with an absence of E4 expression or its focal retention in sporadic cells within the lesion. Our results suggest that a straightforward molecular evaluation of HPV life-cycle deregulation in cervical neoplasia may help improve disease stratification, and that this can be achieved using complementary molecular biomarker pairs such as MCM/E4 or, more promisingly, p16(INK4a)/E4 as an adjunct to conventional pathology.JD, HG, YS, and ZW were funded by the UK Medical Research Council. RvB was funded by the Stichting Pathologie Ontwikkeling en Onderzoek (SPOO) Foundation, The Netherlands.This is the author accepted manuscript. The final version is available via NPG at http://www.nature.com/modpathol/journal/vaop/ncurrent/full/modpathol201552a.html#ack

USP1 Promotes Cholangiocarcinoma Progression by Deubiquitinating PARP1 To Prevent Its Proteasomal Degradation

Despite its involvement in various cancers, the function of the deubiquitinase USP1 (ubiquitin-specific protease 1) is unexplored in cholangiocarcinoma (CCA). In this study, we provide evidence that USP1 promotes CCA progression through the stabilization of Poly (ADP-ribose) polymerase 1 (PARP1), consistent with the observation that both USP1 and PARP1 are upregulated in human CCA. Proteomics and ubiquitylome analysis of USP1-overexpressing CCA cells nominated PARP1 as a top USP1 substrate. Indeed, their direct interaction was validated by a series of immunofluorescence, co-immunoprecipitation (CO-IP), and GST pull-down assays, and their interaction regions were identified using deletion mutants. Mechanistically, USP1 removes the ubiquitin chain at K197 of PARP1 to prevent its proteasomal degradation, with the consequent PARP1 stabilization being necessary and sufficient to promote the growth and metastasis of CCA in vitro and in vivo. Additionally, we identified the acetyltransferase GCN5 as acetylating USP1 at K130, enhancing the affinity between USP1 and PARP1 and further increasing PARP1 protein stabilization. Finally, both USP1 and PARP1 are significantly associated with poor survival in CCA patients. These findings describe PARP1 as a novel deubiquitination target of USP1 and a potential therapeutic target for CCA