An integrated molecular cytogenetic map of Cucumis sativus L. chromosome 2

<p>Abstract</p> <p>Background</p> <p>Integration of molecular, genetic and cytological maps is still a challenge for most plant species. Recent progress in molecular and cytogenetic studies created a basis for developing integrated maps in cucumber (<it>Cucumis sativus </it>L.).</p> <p>Results</p> <p>In this study, eleven fosmid clones and three plasmids containing 45S rDNA, the centromeric satellite repeat Type III and the pericentriomeric repeat CsRP1 sequences respectively were hybridized to cucumber metaphase chromosomes to assign their cytological location on chromosome 2. Moreover, an integrated molecular cytogenetic map of cucumber chromosomes 2 was constructed by fluorescence <it>in situ </it>hybridization (FISH) mapping of 11 fosmid clones together with the cucumber centromere-specific Type III sequence on meiotic pachytene chromosomes. The cytogenetic map was fully integrated with genetic linkage map since each fosmid clone was anchored by a genetically mapped simple sequence repeat marker (SSR). The relationship between the genetic and physical distances along chromosome was analyzed.</p> <p>Conclusions</p> <p>Recombination was not evenly distributed along the physical length of chromosome 2. Suppression of recombination was found in centromeric and pericentromeric regions. Our results also indicated that the molecular markers composing the linkage map for chromosome 2 provided excellent coverage of the chromosome.</p

Simulation of Fragmentation Characteristics of Projectile Jacket Made of Tungsten Alloy after Penetrating Metal Target Plate using SPH Method

A smooth particle hydrodynamics (SPH) model was used to simulate the fragmentation process of the jacket during penetrator with lateral efficiency (PELE) penetrating the metal target plate to study the fragmentation characteristics of PELE jacket made of tungsten alloy. The validity of the SPH model was verified by experimental results. Then the SPH model was used to simulate the jacket fragmentation under different impact velocity and thickness of target plate. The influence of impact velocity and thickness of target plate on the jacket fragmentation was obtained by analysing the mass distribution and quantity distribution of the fragments formed by the jacket. The results show that the dynamic fragmentation of tungsten alloy can be simulated effectively using the SPH model, Johnson-Cook strength model, maximum tensile stress failure criterion and stochastic failure model. When the thickness of target plate is fixed, the greater the impact velocity, the greater the pressure produced by the projectile impacting the target plate; with the increase of impact velocity, the mass of residual projectile decreases, the number of fragments formed by fragmentation of jacket increases linearly, and the average mass of fragments decreases exponentially. When the impact velocity is constant, the greater the thickness of the target plate, the longer the pressure duration by the projectile impacting the target plate; with the increase of the thickness of target plate, the mass of residual projectile decreases, the number of fragments formed by fragmentation of jacket increases linearly, and the average mass of fragments decreases exponentially. The numerical calculation model and research method adopted in this paper can be used to study the impact fragmentation of solid materials effectively

Fragmentation Behaviour of Radial Layered PELE Impacting Thin Metal Target Plates

The fragmentation mechanism of the penetrator with lateral effect (PELE) after perforating a thin target plate has been summarised and analysed firstly. Then the fragmentation of radial layered PELE was analysed qualitatively and verified by experiment. In the experiment, the target plates were made of 45# steel and 2A12 aluminium respectively. Qualitative analysis and experimental results show that: for normal PELE without layered, after perforating the thin metal target plate, from the bottom to the head of the projectile, the number of fragments formed by the jacket gradually increases, and the mass of the fragment decreases correspondingly. Compared with the normal PELE without layered, the radial layered PELE is less likely to break into fragments, when impacting the thin metal target plate with the same material and thickness under the same impact velocity. However, from the mechanism of the PELE, when the resistance of the target plate is large enough, and the duration of pressure is long enough, the radial layered PELE also can break into fragments with transverse velocity component. The resistance of the target plate plays an important role in the fragmentation of radial layered PELE. The radial layered PELE produced massive fragments with transverse velocity component when impacting the 45# steel plate with5 mm thickness under the impact velocity of 657.2 m/s