The Changing Stereotypes of Librarian in Chinese Movies

The representations of librarian in movies which is a main kind of media directly reflect and influence the audiences’ opinions on this profession. This paper analyzed the changing stereotypes of librarian from several aspects by the method of context analysis, and found out that with the information era coming, there presentations of librarian in the movie is also generally changing. These findings can help librarians explore and rethink the self-marketing and self-shaping

The influence of survivin shRNA on the cell cycle and the invasion of SW480 cells of colorectal carcinoma

<p>Abstract</p> <p>Background</p> <p>The objective was to understand the influence of Survivin plasmid with short hairpin RNA (shRNA) on the cell cycle, invasion, and the silencing effect of Survivin gene in the SW480 cell of colorectal carcinoma.</p> <p>Methods</p> <p>A eukaryotic expression vector, PGCH1/Survivin shRNA, a segment sequence of Survivin as target, was created and transfected into colorectal carcinoma cell line SW480 by the non-lipid method. The influence on the Survivin protein was analyzed by Western blotting, while the cell cycle, cell apoptosis were analyzed by flow cytometry, and invasion of the cell was analyzed by Transwell's chamber method.</p> <p>Results</p> <p>After the transfection of PGCH1/Survivin shRNA, the expression of Survivin protein in SW480 cells was dramatically decreased by 60.68%, in which the cells were stopped at G2/M phase, even though no apoptosis was detected. The number of transmembranous cells of the experimental group, negative control group, and blank control group were 14.46 ± 2.11, 25.12 ± 8.37, and 25.86 ± 7.45, respectively (P <<it>0.05</it>).</p> <p>Conclusion</p> <p>Survivin shRNA could significantly reduce the expression of Survivin protein and invasion of SW480 cells. Changes in cell cycle were observed, but no apoptosis was induced.</p

Improvement of Accuracy in Damage Localization Using Frequency Slice Wavelet Transform

Damage localization is a primary objective of damage identification. This paper presents damage localization in beam structure using impact-induced Lamb wave and Frequency Slice Wavelet Transform (FSWT). FSWT is a new time-frequency analysis method and has the adaptive resolution feature. The time-frequency resolution is a vital factor affecting the accuracy of damage localization. In FSWT there is a unique parameter controlling the time-frequency resolution. To improve the accuracy of damage localization, a generalized criterion is proposed to determine the parameter value for achieving a suitable time-frequency resolution. For damage localization, the group velocity dispersion curve (GVDC) of A0 Lamb waves in beam is first accurately estimated using FSWT, and then the arrival times of reflection wave from the crack for some individual frequency components are determined. An average operation on the calculated propagation distance is then performed to further improve the accuracy of damage localization

Frequency slice algorithm for modal signal separation and damping identification

The present study examined in case of papillary renal cell carcinoma (pRCC) whether there consists a correlation between kind and number of the cytogenetic changes and clinical progress, in order to win conclusions of the prognostic relevance of the cytogenetic. Material and methods: Between 1988 and 1997 altogether 31 pRCC of 30 patients were cytogenetically analyzed by short time cultures. The histomorphologic grading oriented itself at the classification of Thoenes et al. (1991) and at the WHO classification for epithelial RCC (Mostofi 1997). In 24 cases the samples have been preparated by radical nephrectomies, in 6 cases by partial resection and in one case the tissue originated from a lymph node. The follow-up data ended in 2001. Results: The cytogenetic dressing showed that both primary and secondary aberrations were clearly presented.. The chromosomes 4 and 10 were involved in no case of the 31 pRCC. There has been no remarkable association of the tumor size and aberration of certain chromosomes. Some authors describe a correlation between trisomy 16 and a higher malignant tendency of the tumor. This could not be verified in this study. With two exceptions all patients with well differentiated pRCC showed complete remission independent from the tumor size. In one case a local lesion after enukleation was observed. Seven of the 30 patients died, 2 of them reliably because of the pRCC. Nine of the 30 patients developed a malignant second desease. Discussion: The aberrations followed a chronological sequence, but did not present a correlation between tumor-size, quantity or quality of the aberrations and the clinical progress. The difficulty to evaluate the progress consisted of the fact that the patients immediately after diagnosis have been treated by surgery. This examination did not demonstrate that the cytogenetic obtains prognostic relevance

Redundancy Removing Aggregation Network with Distance Calibration for Video Face Recognition

Attention-based techniques have been successfully used for rating image quality, and have been widely employed for set-based face recognition. Nevertheless, for video face recognition, where the base convolutional neural network (CNN) trained on large-scale data already provides discriminative features, fusing features with only predicted quality scores to generate representation are likely to cause duplicate sample dominant problem, and degrade performance correspondingly. To resolve the problem mentioned above, we propose a redundancy removing aggregation network (RRAN) for video face recognition. Compared with other quality-aware aggregation schemes, RRAN can take advantage of similarity information to tackle the noise introduced by redundant video frames. By leveraging metric learning, RRAN introduces a distance calibration scheme to align distance distributions of negative pairs of different video representations, which improves the accuracy under a uniform threshold. A series of experiments is conductedon multiple realistic data sets to evaluate the performance of RRAN, including YouTube Faces, IJB-A, and IJB-C. In comprehensive experiments, we demonstrate that our method can diminish the overall influence of poor quality components with large proportion in the video and further improve the overall recognition performance with individual difference. Specifically, RRAN achieves a 96.84% accuracy on YouTube Face, outperforming all existing aggregation schemes.Peer reviewe

Induction of Apoptosis in Human Promyelocytic Leukemia HL60 Cells by Panaxynol and Panaxydol

Panaxynol and panaxydol are naturally occurring polyacetylenes, isolated from the lipophilic fractions of Panax notoginseng, that exert anti-proliferative effects against malignant cells. However, to the best of our knowledge, no study concerning the inhibitory effects of the two polyacetylenes on cell growth of human promyelocytic leukemia cells has been reported. In this paper, we examined the antiproliferation and proapoptotic effects of panaxynol and panaxydol on HL60 cells and investigated their mechanism of action. Cell growth inhibition of panaxynol and panaxydol were determined by trypan blue dye exclusion assays. Apoptosis of cells was revealed by morphological observation, analysis for nuclear DNA distribution and by annexin V-FITC/ PI staining using flow cytometry. It was found that panaxynol and panaxydol markedly inhibited proliferation of HL60 cells in a time- and dose-dependent manner via an apoptotic pathway. In concern with these findings, Western blot analysis showed proteolytic activation of PKCδ, caspase-3 activation and cleavage of poly (ADP [adenosine diphosphate]-ribose) polymerase in HL60 cells treated by panaxynol and panaxydol. In conclusion, panaxynol and panaxydol have profound effects on growth and apoptosis of HL60 cells, suggesting those substances are worthy of further exploration as potential anti-cancer agents