Multiple Conformations of Phosphodiesterase-5: IMPLICATIONS FOR ENZYME FUNCTION AND DRUG DEVELOPMENT

Phosphodiesterase-5 (PDE5) is the target for sildenafil, vardenafil, and tadalafil, which are drugs for treatment of erectile dysfunction and pulmonary hypertension. We report here the crystal structures of a fully active catalytic domain of unliganded PDE5A1 and its complexes with sildenafil or icarisid II. These structures together with the PDE5A1-isobutyl-1-methylxanthine complex show that the H-loop ( residues 660-683) at the active site of PDE5A1 has four different conformations and migrates 7-35 angstrom upon inhibitor binding. In addition, the conformation of sildenafil reported herein differs significantly from those in the previous structures of chimerically hybridized or almost inactive PDE5. Mutagenesis and kinetic analyses confirm that the H-loop is particularly important for substrate recognition and that invariant Gly(659), which immediately precedes the H-loop, is critical for optimal substrate affinity and catalytic activity

Study of Absorbing CO2 from Emissions Using a Spray Tower

In order to reduce the environmental impact caused by CO2 emissions from ships and achieve the goal of green shipping, a spray tower using NaOH solution for the absorption of CO2 has been established in this paper. Using the characteristics of a 6135G128ZCa marine diesel engine, the CO2 absorption system was designed and mathematical models of CO2 absorption efficiency were developed. The effects of the variation in engine exhaust gas temperature, the concentration of NaOH solution, the exhaust gas velocity, different load conditions, and different nozzle types on the absorption efficiency of CO2 were thoroughly investigated experimentally. Moreover, the mechanism of CO2 absorption was analyzed. The developed model was verified by comparing the test results with the simulation results. The results of the study proved that using NaOH solution to absorb CO2 from ship exhausts could reduce the level of CO2 emissions from ships by more than 20%, which indicates that this technology could be used in the future to reduce the level of CO2 emissions from ships

Facile Construction and Fabrication of a Superhydrophobic and Super Oleophilic Stainless Steel Mesh for Separation of Water and Oil

The fluoride-free fabrication of superhydrophobic materials for the separation of oil/water mixtures has received widespread attention because of frequent offshore oil exploration and chemical leakage. In recent years, oil/water separation materials, based on metal meshes, have drawn much attention, with significant advantages in terms of their high mechanical strength, easy availability, and long durability. However, it is still challenging to prepare superhydrophobic metal meshes with high-separation capacity, low costs, and high recyclability for dealing with oil–water separation. In this work, a superhydrophobic and super oleophilic stainless steel mesh (SSM) was successfully prepared by anchoring Fe2O3 nanoclusters (Fe2O3-NCs) on SSM via the in-situ flame synthesis method and followed by further modification with octadecyltrimethoxysilane (OTS). The as-prepared SSM with Fe2O3-NCs and OTS (OTS@Fe2O3-NCs@SSM) was confirmed by a field emission scanning electron microscope (FESEM), transmission electron microscope (TEM), energy dispersive spectrometer (EDS), X-ray photoelectron spectrometer (XPS), and X-ray diffractometer (XRD). The oil–water separation capacity of the sample was also measured. The results show that the interlaced and dense Fe2O3-NCs, composed of Fe2O3 nanoparticles, were uniformly coated on the surface of the SSM after the immerging-burning process. Additionally, a compact self-assembled OTS layer with low surface energy is coated on the surface of Fe2O3-NCs@SSM, leading to the formation of OTS@Fe2O3-NCs@SSM. The prepared OTS@Fe2O3-NCs@SSM shows excellent superhydrophobicity, with a water static contact angle of 151.3°. The separation efficiencies of OTS@Fe2O3-NCs@SSM for the mixtures of oil/water are all above 98.5%, except for corn oil/water (97.5%) because of its high viscosity. Moreover, the modified SSM exhibits excellent stability and recyclability. This work provides a facile approach for the preparation of superhydrophobic and super oleophilic metal meshes, which will lead to advancements in their large-scale applications on separating oil/water mixtures

Fabrication of Carbon Aerogels Derived from Metal-Organic Frameworks/Carbon Nanotubes/Cotton Composites as an Efficient Sorbent for Sustainable Oil–Water Separation

Due to the continuous occurrence of water pollution problems, practical separation methods for oil–water mixtures have attracted more and more attention. To date, different kinds of materials have been developed with good hydrophobic properties and strong separation ability. Carbon aerogels, as a promising ideal adsorbent for dealing with oil-spill accidents, have received extensive attention. In this work, zeolitic imidazolate frameworks (ZIFs), nanoparticles, and carbon nanotubes (CNTs) in the three-dimensional (3D) interconnected network structure of cotton balls (CBs) were successfully prepared by a simple and scalable process. The as-prepared carbonized CBs with carbonized ZIF-8 and CNTs (CZIF-8/CNTs/CCBs) were characterized. The oil–water separation performance of the composite was also measured. The results show that the ZIF-8 clusters intercalated with abundant CNTs are fully loaded into the porous structure of the CBs after the in situ synthesis process. Additionally, ZIF-8/CNTs/CBs was carbonized in nitrogen, leading to the formation of CZIF-8/CNTs/CCBs. The prepared material possesses excellent hydrophobicity with a water contact angle of 152.7°, showing good absorption capacities Q1 in the range of 48 to 84 times its original weight for oil and organic liquids. In addition, CZIF-8/CNTs/CCBs exhibits good recyclability in the absorption–distillation test. In summary, this study proposes a novel and simple method for the preparation of a superhydrophobic material that could have wide application in the separation of oil–water mixtures

Fabrication of Carbon Aerogels Derived from Metal-Organic Frameworks/Carbon Nanotubes/Cotton Composites as an Efficient Sorbent for Sustainable Oil–Water Separation

Due to the continuous occurrence of water pollution problems, practical separation methods for oil–water mixtures have attracted more and more attention. To date, different kinds of materials have been developed with good hydrophobic properties and strong separation ability. Carbon aerogels, as a promising ideal adsorbent for dealing with oil-spill accidents, have received extensive attention. In this work, zeolitic imidazolate frameworks (ZIFs), nanoparticles, and carbon nanotubes (CNTs) in the three-dimensional (3D) interconnected network structure of cotton balls (CBs) were successfully prepared by a simple and scalable process. The as-prepared carbonized CBs with carbonized ZIF-8 and CNTs (CZIF-8/CNTs/CCBs) were characterized. The oil–water separation performance of the composite was also measured. The results show that the ZIF-8 clusters intercalated with abundant CNTs are fully loaded into the porous structure of the CBs after the in situ synthesis process. Additionally, ZIF-8/CNTs/CBs was carbonized in nitrogen, leading to the formation of CZIF-8/CNTs/CCBs. The prepared material possesses excellent hydrophobicity with a water contact angle of 152.7°, showing good absorption capacities Q1 in the range of 48 to 84 times its original weight for oil and organic liquids. In addition, CZIF-8/CNTs/CCBs exhibits good recyclability in the absorption–distillation test. In summary, this study proposes a novel and simple method for the preparation of a superhydrophobic material that could have wide application in the separation of oil–water mixtures

LncRNA HOTAIR is a Prognostic Biomarker for the Proliferation and Chemoresistance of Colorectal Cancer via MiR-203a-3p-Mediated Wnt/ß-Catenin Signaling Pathway

Background/Aims: HOX transcript antisense RNA (HOTAIR) plays a vital role in carcinogenesis. However, its functional and regulatory roles remain unclear. In this study, we aimed to investigate its biological function and clinical significance in human colorectal cancer (CRC). Methods: We examined the expression levels of lncRNA HOTAIR and miR-203a-3p in CRC tissues and CRC cell lines by qRT-PCR. Gain and loss-of-function assays were performed to examine the effects of HOTAIR and miR-203a-3p on the proliferation and chemoresistance of CRC cells. The possible mechanisms of HOTAIR were also explored by fluorescence reporter assay and Western blot. Results: The expressions of HOTAIR were upregulated in CRC tissue tissues compared to adjacent control tissues. We also found HOTAIR was downregulated by miR-203a-3p in CRC cell lines. Both HOTAIR knockdown and miR-203a-3p overexpression in CRC cell lines led to inhibited cell proliferation and reduced chemoresistance. We also determined that β-catenin and GRG5 were inhibitory targets of miR-203a-3p, and that Wnt/β-catenin signaling was inhibited by both HOTAIR knockdown and miR-203a-3p overexpression. Significantly, we found that increased expression of miR-203a-3p is essential for cell proliferation repression, chemoresistance reduction, and Wnt/β-catenin signaling inhibition induced by HOTAIR knockdown. Conclusions: Our study demonstrated that the lncRNA HOTAIR could regulate the progression and chemoresistance of CRC via modulating the expression levels of miR-203a-3p and the activity of Wnt/β-catenin signaling pathway