Statistical Estimation of Root Mean Square Crosstalk in SFP+ Cable Evaluations

A rigorous statistical estimation of the root mean square equation is proposed for near-end crosstalk simulation in SFP+ cable evaluations. This method employs the pulse response, the near-end output in the victim pair due to a single-pulse input of one bit long in the aggressor pair. This pulse response can be obtained from vector network analyzer (VNA) measurements. Thus SFP+ cable evaluations can be effectively performed using easier and more accurate frequency-domain measurements, instead of the time-domain ones defined in the specification. © 2011 IEEE

Using TWDP to Quantify Channel Performance with Frequency-Domain S-Parameter Data

This paper presents an approach to quantify channel performance using TWDP (Transmitter Waveform and Dispersion Penalty) with frequency-domain S-parameter data. TWDP is initially defined to characterize the performance of a transmitter in optical links. The same concept has been extended to quantify channel performance as well, especially in high-speed copper links. This paper focuses on channel characterization. Instead of using time-domain oscilloscope measurements as defined in the original approach, a new method is proposed by using the frequency-domain S-parameter data, obtained either from measurements or simulations. A parametric study on TWDP with respect to bit rate, number of samples per bit, rise/fall time, etc., is also presented with discussions

Granulocyte colony-stimulating factor affects the distribution and clonality of TRGV and TRDV repertoire of T cells and graft-versus-host disease

<p>Abstract</p> <p>Background</p> <p>The immune modulatory effect of granulocyte colony-stimulating factor (G-CSF) on T cells resulted in an unexpected low incidence of graft-versus-host disease (GVHD) in allogeneic peripheral blood stem cell transplantation (allo-PBSCT). Recent data indicated that gamma delta⁺T cells might participate in mediating graft-versus-host disease (GVHD) and graft-versus-leukemia (GVL) effect after allogeneic hematopoietic stem cell transplantation. However, whether G-CSF could influence the T cell receptors (TCR) of gamma delta⁺T cells (<it>TRGV </it>and <it>TRDV </it>repertoire) remains unclear. To further characterize this feature, we compared the distribution and clonality of <it>TRGV </it>and <it>TRDV </it>repertoire of T cells before and after G-CSF mobilization and investigated the association between the changes of TCR repertoire and GVHD in patients undergoing G-CSF mobilized allo-PBSCT.</p> <p>Methods</p> <p>The complementarity-determining region 3 (CDR3) sizes of three <it>TRGV </it>and eight <it>TRDV </it>subfamily genes were analyzed in peripheral blood mononuclear cells (PBMCs) from 20 donors before and after G-CSF mobilization, using RT-PCR and genescan technique. To determine the expression levels of <it>TRGV </it>subfamily genes, we performed quantitative analysis of <it>TRGV</it>I~III subfamilies by real-time PCR.</p> <p>Results</p> <p>The expression levels of three <it>TRGV </it>subfamilies were significantly decreased after G-CSF mobilization (<it>P </it>= 0.015, 0.009 and 0.006, respectively). The pattern of <it>TRGV </it>subfamily expression levels was <it>TRGV</it>II ><it>TRGV </it>I ><it>TRGV </it>III before mobilization, and changed to <it>TRGV </it>I ><it>TRGV </it>II ><it>TRGV </it>III after G-CSF mobilization. The expression frequencies of <it>TRGV </it>and <it>TRDV </it>subfamilies changed at different levels after G-CSF mobilization. Most <it>TRGV </it>and <it>TRDV </it>subfamilies revealed polyclonality from pre-G-CSF-mobilized and G-CSF-mobilized samples. Oligoclonality was detected in <it>TRGV </it>and <it>TRDV </it>subfamilies in 3 donors before mobilization and in another 4 donors after G-CSF mobilization, distributed in <it>TRGV</it>II, <it>TRDV</it>1, <it>TRDV</it>3 and <it>TRDV</it>6, respectively. Significant positive association was observed between the invariable clonality of <it>TRDV</it>1 gene repertoire after G-CSF mobilization and low incidence of GVHD in recipients (<it>P </it>= 0.015, <it>OR </it>= 0.047).</p> <p>Conclusions</p> <p>G-CSF mobilization not only influences the distribution and expression levels of <it>TRGV </it>and <it>TRDV </it>repertoire, but also changes the clonality of gamma delta⁺T cells. This alteration of <it>TRGV </it>and <it>TRDV </it>repertoire might play a role in mediating GVHD in G-CSF mobilized allo-PBSCT.</p

Fast Impedance Prediction for Power Distribution Network using Deep Learning

Modeling and simulating a power distribution network (PDN) for printed circuit boards with irregular board shapes and multi-layer stackup is computationally inefficient using full-wave simulations. This paper presents a new concept of using deep learning for PDN impedance prediction. A boundary element method (BEM) is applied to efficiently calculate the impedance for arbitrary board shape and stackup. Then over one million boards with different shapes, stackup, integrated circuits (IC) location, and decap placement are randomly generated to train a deep neural network (DNN). The trained DNN can predict the impedance accurately for new board configurations that have not been used for training. The consumed time using the trained DNN is only 0.1 s, which is over 100 times faster than the BEM method and 10 000 times faster than full-wave simulations

Long first exons and epigenetic marks distinguish conserved pachytene piRNA clusters from other mammalian genes

In the male germ cells of placental mammals, 26-30-nt-long PIWI-interacting RNAs (piRNAs) emerge when spermatocytes enter the pachytene phase of meiosis. In mice, pachytene piRNAs derive from ~100 discrete autosomal loci that produce canonical RNA polymerase II transcripts. These piRNA clusters bear 5\u27 caps and 3\u27 poly(A) tails, and often contain introns that are removed before nuclear export and processing into piRNAs. What marks pachytene piRNA clusters to produce piRNAs, and what confines their expression to the germline? We report that an unusually long first exon ( \u3e /= 10 kb) or a long, unspliced transcript correlates with germline-specific transcription and piRNA production. Our integrative analysis of transcriptome, piRNA, and epigenome datasets across multiple species reveals that a long first exon is an evolutionarily conserved feature of pachytene piRNA clusters. Furthermore, a highly methylated promoter, often containing a low or intermediate level of CG dinucleotides, correlates with germline expression and somatic silencing of pachytene piRNA clusters. Pachytene piRNA precursor transcripts bind THOC1 and THOC2, THO complex subunits known to promote transcriptional elongation and mRNA nuclear export. Together, these features may explain why the major sources of pachytene piRNA clusters specifically generate these unique small RNAs in the male germline of placental mammals

Averaged Behavior Model of Current-Mode Buck Converters for Transient Power Noise Analysis

Accurate Evaluation and Simulation of Power Noise is Critical in the Development of Modern Electronic Devices. However, the Widely Used Target Impedance Fails to Predict the Low-Frequency Noise Generated in a Device Due to the Existence of the Dc–dc Converter, Whose Output Impedance Can Change under Different Loading Conditions. a Physical Circuit Model is Then Desired to Replicate the Behavior of a Voltage Regulator Module, and the Average Technique is an Efficient Method to Estimate the Noise of a Pulse Width-Modulated (PWM) Converter. with the Emergence of Converters with Adaptive On-Time (AOT) Controllers, More Complex Averaging Methods Are Required, But None of Them Supports Transient Simulation. a General, Efficient, and Accurate Modeling Technique is Presented in This Article, Whose Framework Supports Both Current-Mode PWM and AOT Controllers. in Addition, a Novel Two-Step Parameter Extraction Method is Proposed, Which Can Be Used to Evaluate the Equivalent Values of Internal Feedback Parameters of an Encrypted Simulation Model or from Measurement. the Modeling Method is Validated by Both Simulation and Measurement

A Large Portal Vein: A Rare Finding of Recent Portal Vein Thrombosis

Acute portal vein thrombosis (PVT) is rarely encountered by clinicians. The most common manifestation of acute PVT is sudden onset of abdominal pain. A computed tomography scan without contrast often shows a high-density material in the portal vein. After injection of contrast agents, absence of luminal enhancement and enlargement of the obstructed portal vein are shown. In this case report, we demonstrated a rare computed tomography finding in which the diameter of the main portal vein was enormously distended to 3-fold that of the aorta in a patient with recent PVT. Despite thrombolysis and anticoagulation were immediately given, portal venous recanalization was not achieved in the patient. After 5 years, variceal bleeding and ascites occurred and liver function had persistently deteriorated. Finally, he died of progressive liver failure. Considering this case, we suggest that an early decision for invasive interventional treatment might be necessary to both increase the rate of portal venous recanalization and improve prognosis, as anticoagulation and thrombolysis therapy failed to recanalize recent PVT

Theoretical and experimental investigation on backward-pumped Yb(3+)-doped double-clad fiber lasers

Based on the rate equations under the steady-state condition, strongly backward-pumped Yb(3+)-doped double-clad fiber (YDCF) lasers are analyzed numerically. The effects of laser cavity feedbacks and length of YDCF on the laser performance and the dependency of the output laser power on the pump power are investigated. Using a 975 nm laser diode (LD) as the pump source, a backward-pumped YDCF laser with a slope efficiency of 82% is demonstrated experimentally. The output laser power at the wavelength of 1088 nm is up to 85 W. The experimental results are in good agreement with those by numerical simulations

The HP1 Homolog Rhino Anchors a Nuclear Complex that Suppresses piRNA Precursor Splicing

SummarypiRNAs guide an adaptive genome defense system that silences transposons during germline development. The Drosophila HP1 homolog Rhino is required for germline piRNA production. We show that Rhino binds specifically to the heterochromatic clusters that produce piRNA precursors, and that binding directly correlates with piRNA production. Rhino colocalizes to germline nuclear foci with Rai1/DXO-related protein Cuff and the DEAD box protein UAP56, which are also required for germline piRNA production. RNA sequencing indicates that most cluster transcripts are not spliced and that rhino, cuff, and uap56 mutations increase expression of spliced cluster transcripts over 100-fold. LacI::Rhino fusion protein binding suppresses splicing of a reporter transgene and is sufficient to trigger piRNA production from a trans combination of sense and antisense reporters. We therefore propose that Rhino anchors a nuclear complex that suppresses cluster transcript splicing and speculate that stalled splicing differentiates piRNA precursors from mRNAs