GeoViewMatch: A Multi-Scale Feature-Matching Network for Cross-View Geo-Localization Using Swin-Transformer and Contrastive Learning

Cross-view geo-localization aims to locate street-view images by matching them with a collection of GPS-tagged remote sensing (RS) images. Due to the significant viewpoint and appearance differences between street-view images and RS images, this task is highly challenging. While deep learning-based methods have shown their dominance in the cross-view geo-localization task, existing models have difficulties in extracting comprehensive meaningful features from both domains of images. This limitation results in not establishing accurate and robust dependencies between street-view images and the corresponding RS images. To address the aforementioned issues, this paper proposes a novel and lightweight neural network for cross-view geo-localization. Firstly, in order to capture more diverse information, we propose a module for extracting multi-scale features from images. Secondly, we introduce contrastive learning and design a contrastive loss to further enhance the robustness in extracting and aligning meaningful multi-scale features. Finally, we conduct comprehensive experiments on two open benchmarks. The experimental results have demonstrated the superiority of the proposed method over the state-of-the-art methods

Predicted Range Aggregate Processing in Spatio-temporal Databases

Predicted range aggregate (PRA) query is an important researching issue in spatio-temporal databases. Recent studies have developed two major classes of PRA query methods: (1) accurate approaches, which search the common moving objects indexes to obtain an accurate result; and (2) estimate methods, which utilize approximate techniques to estimate the result with an acceptable error. In this paper, we present a novel accurate prediction index technique, named PRA-tree, for range aggregation of moving objects. PRA-tree takes into account both the velocity and space distribution of moving objects. First, the velocity domain is partitioned into different velocity buckets, and moving objects are classified into different velocity buckets by their velocities, thus objects in one bucket have similar velocities. Then we use aTPR-tree, which is based on the basic TPR-tree structure and added with aggregate information in intermediate nodes, to index objects in each bucket. PRA-tree is supplemented by a hash index on IDs of moving objects, and exploits bottom-up deletion algorithm, thus having a good dynamic performance and concurrency. Also new PRA query methods with a more precise branch-andbound searching strategy are developed for PRAtree. Extensive experiments confirm that the proposed methods are efficient and practical. 1

Guidelines for the use and interpretation of assays for monitoring autophagy

In 2008 we published the first set of guidelines for standardizing research in autophagy. Since then, research on this topic has continued to accelerate, and many new scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Accordingly, it is important to update these guidelines for monitoring autophagy in different organisms. Various reviews have described the range of assays that have been used for this purpose. Nevertheless, there continues to be confusion regarding acceptable methods to measure autophagy, especially in multicellular eukaryotes. A key point that needs to be emphasized is that there is a difference between measurements that monitor the numbers or volume of autophagic elements (e.g., autophagosomes or autolysosomes) at any stage of the autophagic process vs. those that measure flux through the autophagy pathway (i.e., the complete process); thus, a block in macroautophagy that results in autophagosome accumulation needs to be differentiated from stimuli that result in increased autophagic activity, defined as increased autophagy induction coupled with increased delivery to, and degradation within, lysosomes (in most higher eukaryotes and some protists such as Dictyostelium) or the vacuole (in plants and fungi). In other words, it is especially important that investigators new to the field understand that the appearance of more autophagosomes does not necessarily equate with more autophagy. In fact, in many cases, autophagosomes accumulate because of a block in trafficking to lysosomes without a concomitant change in autophagosome biogenesis, whereas an increase in autolysosomes may reflect a reduction in degradative activity. Here, we present a set of guidelines for the selection and interpretation of methods for use by investigators who aim to examine macroautophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes. These guidelines are not meant to be a formulaic set of rules, because the appropriate assays depend in part on the question being asked and the system being used. In addition, we emphasize that no individual assay is guaranteed to be the most appropriate one in every situation, and we strongly recommend the use of multiple assays to monitor autophagy. In these guidelines, we consider these various methods of assessing autophagy and what information can, or cannot, be obtained from them. Finally, by discussing the merits and limits of particular autophagy assays, we hope to encourage technical innovation in the field

Guidelines for the use and interpretation of assays for monitoring autophagy

In 2008 we published the first set of guidelines for standardizing research in autophagy. Since then, research on this topic has continued to accelerate, and many new scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Accordingly, it is important to update these guidelines for monitoring autophagy in different organisms. Various reviews have described the range of assays that have been used for this purpose. Nevertheless, there continues to be confusion regarding acceptable methods to measure autophagy, especially in multicellular eukaryotes. A key point that needs to be emphasized is that there is a difference between measurements that monitor the numbers or volume of autophagic elements (e.g., autophagosomes or autolysosomes) at any stage of the autophagic process vs. those that measure flux through the autophagy pathway (i.e., the complete process); thus, a block in macroautophagy that results in autophagosome accumulation needs to be differentiated from stimuli that result in increased autophagic activity, defined as increased autophagy induction coupled with increased delivery to, and degradation within, lysosomes (in most higher eukaryotes and some protists such as Dictyostelium) or the vacuole (in plants and fungi). In other words, it is especially important that investigators new to the field understand that the appearance of more autophagosomes does not necessarily equate with more autophagy. In fact, in many cases, autophagosomes accumulate because of a block in trafficking to lysosomes without a concomitant change in autophagosome biogenesis, whereas an increase in autolysosomes may reflect a reduction in degradative activity. Here, we present a set of guidelines for the selection and interpretation of methods for use by investigators who aim to examine macroautophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes. These guidelines are not meant to be a formulaic set of rules, because the appropriate assays depend in part on the question being asked and the system being used. In addition, we emphasize that no individual assay is guaranteed to be the most appropriate one in every situation, and we strongly recommend the use of multiple assays to monitor autophagy. In these guidelines, we consider these various methods of assessing autophagy and what information can, or cannot, be obtained from them. Finally, by discussing the merits and limits of particular autophagy assays, we hope to encourage technical innovation in the field