Kidneys with small renal cancer after perfusion with 4°C UW solution.

<p>All three renal cancers were less than 4.8(ccCRC).</p

Proliferation of HK-2 and 7860 cells after cold-culture in UW solution and recovery in normal conditions.

<p>Cells were cold-cultured in UW solution for the indicated length of time and harvested. Harvested cells were plated onto 96-well plates at 3000 cells per well in complete medium and allowed to grow for 20 h in a 37°C, 5% CO₂ incubator. Subsequently, 10 µl of CCK solution was added to each well and plates were returned to the incubator for another 4 h before the absorbance at 450 nm was measured. *<i>p</i><0.05.</p

Expression of Ki67 following organ preservation.

<p>Protein samples were separated by SDS-PAGE, transferred onto membranes, and probed with anti-Ki67 antibodies. Stripped membranes were re-probed with anti-GAPDH antibodies as a loading control.</p

The differential inhibition of proliferation between HK-2 and 7860 cells in differing culture conditions.

<p>The differential inhibition rate is the difference between inhibition rate of proliferation of 7860 and HK-2 cells by different culture conditions. *<i>p</i><0.05.</p

Cellular proliferation under various experimental conditions.

<p>Proliferation of HK-2 and 7860 cells after: A, cold-culture (4°C) in UW solution; B, cold-culture in normal medium; and C, culture at 37°C in UW solution. Cells were plated onto 96-well plates at 3000 cells per well in complete medium and cultured for 24 h in a 37°C, 5% CO₂ incubator. The culture medium was then changed, and wells were divided equally into four groups. Groups 1, 2, 3, and 4 were cold-cultured in UW solution for 0, 6, 12, and 24 h, respectively, as indicated. Subsequently, 10 µl of CCK solution was added to each well, and plates were returned to the incubator for 4 h before the absorbance at 450 nm was measured. *<i>p</i><0.05.</p

Percentage of viable cells after cold-storage in UW solution or normal saline.

<p>After culture for the indicated length of time, viable and dead cells were counted by Trypan Blue exclusion and microscopy. *<i>p</i><0.05.</p