Research on the ZigBee Network and Equipment Design Based on the CC2530

It is known to us all that embedded systems designed on the 51 chip microcomputer are mature. This research finished to plant the system from 51 chip microcomputer to the CC2530 chip of TI. After analyzing and mastering the ZigBee 2007 PRO protocol stack, a new set of wireless sensor network (WSN) products has been developed. In the system, the coordinator can establish the Mesh network on its own initiative and the child nodes can join the network. The coordinator can be connected to the personal computer or the embedded gateway. The gateway had the Ethernet port and can send the data from the sensors to the cable or wireless network. The operator can issue instructions automatically and manually. The information of the sensor nodes can be returned and displayed on the serial debugging assistant. The equipment has passed the test with different environments in which there are obstacles among the communicating sensors and can be applied in the traffic, environmental monitoring and other fields widely

N-terminal lid swapping contributes to the substrate specificity and activity of thermophilic lipase TrLipE

TrLipE is a thermophilic lipase that has potential commercial applications because of its catalytic ability under extreme conditions. Consistent with most lipases, the lid of TrLipE is located over the catalytic pocket, controls the substrate channel to the active center, and regulates the substrate specificity, activity, and stability of the enzyme through conformational changes. TrLipE from Thermomicrobium roseum has potential industrial applications, which is hindered by its weak enzymatic activity. Here, 18 chimeras (TrL1-TrL18) were reconstructed by N-terminal lid swapping between TrLipE and structurally similar enzymes. The results showed that the chimeras had a similar pH range and optimum pH as wild TrLipE but a narrower temperature range of 40–80°C, and TrL17 and the other chimeras showed lower optimum temperatures of 70°C and 60°C, respectively. In addition, the half-lives of the chimeras were lower than those of TrLipE under optimum temperature conditions. Molecular dynamics simulations indicated that chimeras had high RMSD, RMSF, and B-factor values. When p-nitrophenol esters with different chains were used as substrates, compared with TrLipE, most of the chimeras had a low Km and high kcat value. The chimeras TrL2, TrL3, TrL17, and TrL18 could specifically catalyze the substrate 4-nitrophenyl benzoate, with TrL17 showing the highest kcat/Km value of 363.88 ± 15.83 L⋅min–1⋅mmol–1. Mutants were then designed by investigating the binding free energies of TrL17 and 4-nitrophenyl benzoate. The results indicated that single, double, and triple substitution variants (M89W and I206N; E33W/I206M and M89W/I206M; and M89W/I206M/L21I and M89W/I206N/L21I, respectively) presented approximately 2- to 3-fold faster catalysis of 4-nitrophenyl benzoate than the wild TrL17. Our observations will facilitate the development of the properties and industrial applications of TrLipE

Exploring Proper Spacing Threshold of Non-Submerged Spur Dikes with Ipsilateral Layout

Concerning the clustering of spur dikes on river systems, the spacing thresholds of twin spur dikes are important parameters to influence the estimations on the impact scales of spur dike groups and the overall responses of river systems. In this study, both numerical investigations and experimental measurements are proceeded to quantify the influence of the spacing threshold of non-submerged twin spur dikes with ipsilateral and orthogonal layout in a straight rectangular channel. Through dimensional analysis, three normalized indices, i.e., Froude number Fr, ratios of channel width to dike length B/b, and ratios of channel width to water depth B/h are identified as the main influencing factors of the relative spacing threshold Sc/b, i.e., dike spacing threshold to dike length. The simulation results indicate that the similarity of mean velocity along the water depth nearby the tips of twin spur dikes is determined by the criterion of the spacing threshold of non-submerged twin spur dikes with ipsilateral and orthogonal layout in straight rectangular channel. The results also show that: Fr plays the least impact among the three influencing factors; with the fixed values of Fr and B/h, the relative threshold Sc/b sharply increases first and then decreases slightly as B/b factor increases, with which the relationship presents approximately convex quadratic function; while both Fr and B/b are fixing, the Sc/b changes oppositely, i.e., slightly increasing first and then sharply decreasing as B/h increases, which, again presents a convex quadratic function. Hence, the normalized empirical formula of spacing threshold can be deduced by multivariate regressions and verified by the corresponding measurements in good agreements. Such empirical formula further suggests that the reasonable spacing threshold ranges from 24b to 130b, which is wider than the recovery area scales found in literature. The outputs of this study provide foundation for the characterization of impact scales of spur dike groups

Study on Spacing Threshold of Nonsubmerged Spur Dikes with Alternate Layout

This paper investigated the spacing threshold of nonsubmerged spur dikes with alternate layout to classify the impact scale of spur dikes. A mathematical model was built based on standard k-ε model, finite volume method (FVM), and rigid lid assumption and was verified by experimental data. According to dimensional analysis, three indices, that is, Fr (Froude number), B/b (channel width to dike length), and B/h (channel width to water depth), were identified as the influencing factors on the spacing threshold, based on which fifteen sets of conditions were simulated. The calculation results indicate that B/h is the most influencing parameter on Sc/b (spacing threshold to dike length), followed by B/b and Fr. A dimensionless empirical formula of spacing threshold is fitted by multivariate regression. The results of four sets of additional conditions illustrate that the generalization of empirical formula is satisfactory and the precision of interpolation is higher than that of extrapolation. Furthermore, the spacing threshold of alternate spur dikes is generally smaller than ipsilateral spur dikes

Design and Experiment of Low-Pressure Gas Supply System for Dual Fuel Engine

A low-pressure gas supply system for dual fuel engines was designed to transport liquid natural gas from a storage tank to a dual fuel engine and gasify it during transportation. The heat exchange area and pressure drop in the spiral- wound heat exchanger, the volume of the buffer tank and the pressure drop in the pipeline of the gas supply system were calculated by programming using Python. Experiments were carried out during the proces of starting and running the dual fuel engine using this gas supply system. Experimental data show that the gas supply system can supply gas stably during the process and ensure the stable operation of the dual fuel engine. The effects of the parameters of natural gas and ethylene glycol solution on the heat exchange area of the spiralwound heat exchanger and the volume of the buffer tank in the gas supply system were studied. The results show that the heat exchange area calculated according to pure methane can adapt to the case of non-pure methane. The temperature difference between natural gas and ethylene glycol solution should be increased in order to reduce the heat exchange area. The heat exchange area selected according to the high pressure of natural gas can adapt to the low pressure of natural gas. The volume of the buffer tank should be selected according to the situation of the minimum methane content to adapt to the situation of high methane content. The main influencing factor in selecting the volume of the buffer tank is the natural gas flow. The results can provide guidance for the design of the gas supply system for dual fuel engines

Design and Experiment of Low-Pressure Gas Supply System for Dual Fuel Engine

A low-pressure gas supply system for dual fuel engines was designed to transport liquid natural gas from a storage tank to a dual fuel engine and gasify it during transportation. The heat exchange area and pressure drop in the spiral- wound heat exchanger, the volume of the buffer tank and the pressure drop in the pipeline of the gas supply system were calculated by programming using Python. Experiments were carried out during the process of starting and running the dual fuel engine using this gas supply system. Experimental data show that the gas supply system can supply gas stably during the process and ensure the stable operation of the dual fuel engine. The effects of the parameters of natural gas and ethylene glycol solution on the heat exchange area of the spiral-wound heat exchanger and the volume of the buffer tank in the gas supply system were studied. The results show that the heat exchange area calculated according to pure methane can adapt to the case of non-pure methane. The temperature difference between natural gas and ethylene glycol solution should be increased in order to reduce the heat exchange area. The heat exchange area selected according to the high pressure of natural gas can adapt to the low pressure of natural gas. The volume of the buffer tank should be selected according to the situation of the minimum methane content to adapt to the situation of high methane content. The main influencing factor in selecting the volume of the buffer tank is the natural gas flow. The results can provide guidance for the design of the gas supply system for dual fuel engines

Channel Access and Power Control for Mobile Crowdsourcing in Device-to-Device Underlaid Cellular Networks

With the access of a myriad of smart handheld devices in cellular networks, mobile crowdsourcing becomes increasingly popular, which can leverage omnipresent mobile devices to promote the complicated crowdsourcing tasks. Device-to-device (D2D) communication is highly desired in mobile crowdsourcing when cellular communications are costly. The D2D cellular network is more preferable for mobile crowdsourcing than conventional cellular network. Therefore, this paper addresses the channel access and power control problem in the D2D underlaid cellular networks. We propose a novel semidistributed network-assisted power and a channel access control scheme for D2D user equipment (DUE) pieces. It can control the interference from DUE pieces to the cellular user accurately and has low information feedback overhead. For the proposed scheme, the stochastic geometry tool is employed and analytic expressions are derived for the coverage probabilities of both the cellular link and D2D links. We analyze the impact of key system parameters on the proposed scheme. The Pareto optimal access threshold maximizing the total area spectral efficiency is obtained. Unlike the existing works, the performances of the cellular link and D2D links are both considered. Simulation results show that the proposed method can improve the total area spectral efficiency significantly compared to existing schemes

Laboratory Studies on Nearshore Density-Driven Exchange Flow over a Partly Vegetated Slope

Density-driven exchange flows, which are important to the transport of nutrients, pollutants and chemical substances without external forcing, were studied through laboratory lock-exchange experiments. Rigid and emergent cylinders were placed in one of two reservoirs in a partitioned wedge-shaped tank to simulate a partly vegetated slope. The experimental results found that cylinders placed on only one side of the tank lead to different current speeds in the current head and tail that subsequently create various flow patterns and significantly affect the downslope current motions. By fitting with the experimental data, some unknown coefficients can be obtained in the theoretical formulae that are able to predict the intrusion length and exchange flowrate in real field systems. Compared to the flat bed cases, the total exchange discharge over a steep slope decreases by up to 4% for vegetation distributed in shallow water and increases by 14% for vegetation distributed in deeper regions. These results suggest that bed slope and vegetation distribution are crucial to the density-driven exchange flows in the flushing of nearshore regions

Numerical investigation of silted-up dam-break flow with different silted-up sediment heights

The silted-up sediment in the reservoir may have a significant influence on the propagation of dam-break flows. In this paper, a three-dimensional numerical simulation of the silted-up dam-break flow is carried out. In this paper, simulations of three-dimensional silted-up dam-break flow are carried out. A kind of Eulerian–Eulerian two-fluid model (TFM), coupled level set and volume of fluid (CLSVOF) methods, is presented. In order to calculate the motions of the air–water interface and the sediment simultaneously, kinetic particle theory (KPT) and computational fluid dynamics (CFD) are combined. The rheology-based constitutive equations of sediment are also considered to simulate scouring and deposition. In addition, a partial-slip boundary condition (BC) for the velocity of the sediment phase at stationary walls is implemented. The simulation results of the benchmark case demonstrate that the proposed model can effectively simulate the silted-up dam-break flow while taking into account multi-interface capturing problems. Subsequently, the simulations of the silted-up dam-break flow over dry are investigated numerically in a three-dimensional long channel. The simulated results reveal that, in the dam-break flows, the silted-up sediment height has a significant influence on wave propagation, dynamic pressure loads, sediment transport, and sediment deposition. HIGHLIGHTS Three-dimensional simulation of silted-up dam-break flow is carried out.; The air–water interface movement is captured by CLSVOF method.; A Eulerian–Eulerian multiphase model coupling kinetic particle theory and computational fluid dynamics is used.; The rheology-based constitutive equations of sediment are considered.; The effect of the silted-up sediment height during a dam-break flow is investigated.

A Novel Regulator Participating in Nitrogen Removal Process of Bacillus subtilis JD-014

Aerobic denitrification is considered as a promising biological method to eliminate the nitrate contaminants in waterbodies. However, the molecular mechanism of this process varies in different functional bacteria. In this study, the nitrogen removal characteristics for a newly isolated aerobic denitrifier Bacillus subtilis JD-014 were investigated, and the potential functional genes involved in the aerobic denitrification process were further screened through transcriptome analysis. JD-014 exhibited efficient denitrification performance when having sodium succinate as the carbon source with the range of nitrate concentration between 50 and 300 mg/L. Following the transcriptome data, most of the up-regulated differentially expressed genes (DEGs) were associated with cell motility, carbohydrate metabolism, and energy metabolism. Moreover, gene nirsir annotated as sulfite reductase was screened out and further identified as a regulator participating in the nitrogen removal process within JD-014. The findings in present study provide meaningful information in terms of a comprehensive understanding of genetic regulation of nitrogen metabolism, especially for Bacillus strains