503 research outputs found

    Construction of Sly-miR393 Over-Expression Vector and Verification of Its Target Genes in Tomato

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    To understand the function of Sly-miR393 in tomato, the precursor sequences and potential target genes of Sly-miR393 were identificated from tomato genome database by computational homology search method. The Sly-miR393 gene was amplified from the genomic DNA by PCR and cloned into plant expression vector pLP35s-100. Sly-miR393 guided-cleavage to putative target  transcripts was validated u sing 5RACE RT-PCR. In this study, our results indicated that the precursor sequence of Sly-miR393 contains the complete hairpin  structure. TIR1/AFB auxin receptor genes contain recognition sites with high complementarities to Sly-miR393 sequence. In tomato, Sly-miR393 directs the cleavage of SlTIR1,SlTIR1-like1 and SlAFB mRNA, then auxin receptor homologous was validated to be as target of Sly-miR393. The pLP35s-pre-SlymiR393 vector containing Sly-miR393 gene was successfully constructed, which would provide significant evidence for further study of Sly-miR393 function in auxin signaling pathway in tomato

    Silencing Sl-EBF1 and Sl-EBF2 expression causes constitutive ethylene response phenotype, accelerated plant senescence, and fruit ripening in tomato

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    The hormone ethylene regulates a wide range of plant developmental processes and EBF (EIN3-binding F-box) proteins were shown to negatively regulate the ethylene signalling pathway via mediating the degradation of EIN3/EIL proteins. The present study reports on the identification of two tomato F-box genes, Sl-EBF1 and Sl-EBF2 from the EBF subfamily. The two genes display contrasting expression patterns in reproductive and vegetative tissues and in response to ethylene and auxin treatment. Sl-EBF1 and Sl-EBF2 genes are actively regulated at crucial stages in the development of the reproductive organs. Their dynamic expression in flowers during bud-to-anthesis and anthesis-to-post-anthesis transitions, and at the onset of fruit ripening, suggests their role in situations where ethylene is required for stimulating flower opening and triggering fruit ripening. VIGS-mediated silencing of a single tomato EBF gene uncovered a compensation mechanism that tends to maintain a threshold level of Sl-EBF expression via enhancing the expression of the second Sl-EBF gene. In line with this compensation, tomato plants silenced for either of the Sl-EBF genes were indistinguishable from control plants, indicating functional redundancy among Sl-EBF genes. By contrast, co-silencing of both Sl-EBFs resulted in ethylene-associated phenotypes. While reports on EBF genes to date have focused on their role in modulating ethylene responses in Arabidopsis, the present study uncovered their role in regulating crucial stages of flower and fruit development in tomato. The data support the hypothesis that protein degradation via the ubiquitin/26S proteasome pathway is a control point of fruit ripening and open new leads for engineering fruit quality

    Intensity Mapping Functions For HDR Panorama Imaging: Weighted Histogram Averaging

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    It is challenging to stitch multiple images with different exposures due to possible color distortion and loss of details in the brightest and darkest regions of input images. In this paper, a novel intensity mapping algorithm is first proposed by introducing a new concept of weighted histogram averaging (WHA). The proposed WHA algorithm leverages the correspondence between the histogram bins of two images which are built up by using the non-decreasing property of the intensity mapping functions (IMFs). The WHA algorithm is then adopted to synthesize a set of differently exposed panorama images. The intermediate panorama images are finally fused via a state-of-the-art multi-scale exposure fusion (MEF) algorithm to produce the final panorama image. Extensive experiments indicate that the proposed WHA algorithm significantly surpasses the related state-of-the-art intensity mapping methods. The proposed high dynamic range (HDR) stitching algorithm also preserves details in the brightest and darkest regions of the input images well. The related materials will be publicly accessible at https://github.com/yilun-xu/WHA for reproducible research.Comment: 11 pages, 5 figure
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