Detection of recurrent cytogenetic aberrations in multiple myeloma: A comparison between MLPA and iFISH

Multiple myeloma (MM) is a genetically heterogeneous disease with diverse clinical characteristics and outcomes. Recently, multiplex ligation-dependent probe amplification (MLPA) has emerged as an effective and robust method for the detection of cytogenetic aberrations in MM patients. In the present study, MLPA analysis was applied to analyze cytogenetics of CD138 tumor cells of 59 MM samples, and its result was compared, retrospectively, with the interphase fluorescence in situ hybridization (iFISH) data. We firstly established the normal range of each of the 42 diagnostic probes using healthy donor samples. A total of 151 aberrations were detected in 59 patient samples, and 49/59 cases (83.1%) harbored at least one copy number variation. Overall, 0–7 aberrations were detected per case using MLPA, indicating the heterogeneity and complexity of MM cytogenetics. We showed the high efficiency of MLPA and the high congruency of the two methods to assess cytogenetic aberrations. Considering that MLPA analysis is not reliable when the aberration only exits in a small population of tumor cells, it is essential to use both MLPA and iFISH as complementary techniques for the diagnosis of MM

Increasing Bicycles’ Share of Commuter Trips in Atlanta Through Improved Trip Routing Methodologies

The paper investigates some of the many elements required for successful bicycling. It gives some suggestions to encourage people to choose bicycles as their trip mode. Due to limited data, the paper considers only bike-to-work trips. The report is split into three major sections: workplace incentives and facilities, land-use and bike facilities, and suggestions for bicycle trip routing. For workplace incentives, the analysis found that even though many employers generally offer showers and/or incentives for biking to work, this type of data is not being stored and is thus not widely available. For land-use and bike-facilities, the analysis found that, while multi-use paths and bike lanes generally serve destinations and origins equally, protected facilities are located in more destination- heavy areas. For the third section, the paper puts forward suggestions based on bicycle trip experience, aimed at producing reasonable results. Further, this section lays out the required process for routing bicycle trips. Finally, considering the current special Covid-19 situation, the paper includes some speculations about the future of bicycle use resulting from the epidemic conditions

Assessment of influencing factors on non-point source pollution critical source areas in an agricultural watershed

Critical Source Areas (CSAs) are areas that contribute disproportionate high levels of non-point source (NPS) pollution to receiving waters, and their occurrence is the result of the complex interaction between the factors related to the sources and transport processes of NPS pollution. A systematic understanding of how these influencing factors affect CSAs is essential for successful watershed management. In this study, we applied a statistical data mining technique boosted regression tree model to quantify the contribution of eight influencing factors (soil type, slope, elevation, RUSLE LS factor, RUSLE K factor, runoff, fertilizer application rate and land use) on two types of CSAs (TN-CSAs and TP-CSAs), as well as the marginal effects and potential thresholds of influencing factors on the occurrence of CSAs. Results show that land use (37.35%, 25.03%), fertilizer application (36.93%, 57.83%) and soil type (17.59%, 13.70%) have higher importance in determining the occurrence of TN-CSAs and TP-CSAs; and the incidence of TN-CSAs is positively correlated with most factors before the threshold for each influencing factor, after which the marginal effect largely leveled off or dropped slightly; TP-CSAs have essentially the same characteristics as TN-CSAs, but TP-CSAs are more likely to occur in areas with an annual runoff of around 244.92 mm. In addition, this study discussed the application of machine learning techniques in predicting CSAs under climate change without physical-based models, as well as a preliminary watershed management planning for NPS pollution control in the study watershed. These results provided important information for nutrient management regulations

Ginsenoside Rb3 Inhibits Pro-Inflammatory Cytokines via MAPK/AKT/NF-κB Pathways and Attenuates Rat Alveolar Bone Resorption in Response to Porphyromonas gingivalis LPS

Conventional treatments for chronic periodontitis are less effective in controlling inflammation and often relapse. Therefore, it is necessary to explore an immunomodulatory medication as an adjuvant. Ginsenoside Rb3 (Rb3), one of the most abundant active components of ginseng, has been found to possess anti-inflammatory and immunomodulatory properties. Here, we detected the anti-inflammatory effect of Rb3 on Porphyromonas gingivalis LPS-stimulated human periodontal ligament cells and experimental periodontitis rats for the first time. We found that the expression of pro-inflammatory mediators, including IL-1β, IL-6 and IL-8, upregulated by lipopolysaccharide (LPS) stimulation was remarkably downregulated by Rb3 treatment in a dose-dependent manner at both transcriptional and translational levels. Network pharmacological analysis of Rb3 showed that the mitogen-activated protein kinase (MAPK) signaling pathway had the highest richness and that p38, JNK, and ERK molecules were potential targets of Rb3 in humans. Western blot analysis revealed that Rb3 significantly suppressed the phosphorylation of p38 MAPK and p65 NF-κB, as well as decreased the expression of total AKT. In experimental periodontitis rat models, reductions in alveolar bone resorption and osteoclast generation were observed in the Rb3 treatment group. Thus, we can conclude that Rb3 ameliorated Porphyromonas gingivalis LPS-induced inflammation by inhibiting the MAPK/AKT/NF-κB signaling pathways and attenuated alveolar bone resorption in experimental periodontitis rats

Comparative study on the properties and microscopic mechanism of Ti coating and W coating diamond-copper composites

Interface plays a decisive role in metal matrix composites, and the effects of Ti coating and W coating on the properties and microscopic mechanism of diamond-copper composites are compared in this paper. Ti-coated diamond with 50 nm, 100 nm and 150 nm plating thickness and W-coated diamond with 50 nm plating thickness are prepared by using magnetron sputtering. Then infiltration method is carried out to prepare diamond copper composites. SEM, EDS and XRD are used to material microstructure. Three-point bending experiment and flash method are used to test the bending strength and thermal conductivity of the composite material. The study found that, as the thickness of the Ti coating increases, the bending strength of the composites gradually increases, but the thermal conductivity first increases and then decreases. The thermal conductivity of W coated diamond copper composites is higher than that of Ti coated diamond copper composites with the same coating thickness. But bonding strength shows the opposite law. The reason for the above phenomenon is that the mechanism of action between the Ti coating and the W coating and the copper substrate is different at the micro interface of the composites. The research work has important reference value for the interface modification of diamond copper composites

Effect of trace La on microstructure and properties of Cu–Cr–In alloys

The strength and conductivity of Cu–Cr–In alloys with trace La and different processing states were compared. Then, the influence of La on the structure and properties of the Cu–Cr–In alloy and the associated mechanisms were analyzed. The mechanical properties and microstructural evolution of the alloy were investigated via scanning electron microscopy, transmission electron microscopy, and tensile strength measurements. The results showed that the addition of 0.07wt% La to the Cu–Cr–In alloy had the effect of purifying the alloy melt and removing impurities, which improved the electrical conductivity of the alloy. However, the formation of rare earth intermetallic compounds reduced the tensile strength of the Cu–Cr–In–La alloy. After thermomechanical treatment, the Cu–Cr–In–La alloy reached a peak strength of 457 MPa and a conductivity of 85% IACS

Genome-Wide Analysis of Type-III Polyketide Synthases in Wheat and Possible Roles in Wheat Sheath-Blight Resistance

The enzymes in the chalcone synthase family, also known as type-III polyketide synthases (PKSs), play important roles in the biosynthesis of various plant secondary metabolites and plant adaptation to environmental stresses. There have been few detailed reports regarding the gene and tissue expression profiles of the PKS (TaPKS) family members in wheat (Triticum aestivum L.). In this study, 81 candidate TaPKS genes were identified in the wheat genome, which were designated as TaPKS1–81. Phylogenetic analysis divided the TaPKS genes into two groups. TaPKS gene family expansion mainly occurred via tandem duplication and fragment duplication. In addition, we analyzed the physical and chemical properties, gene structures, and cis-acting elements of TaPKS gene family members. RNA-seq analysis showed that the expression of TaPKS genes was tissue-specific, and their expression levels differed before and after infection with Rhizoctonia cerealis. The expression levels of four TaPKS genes were also analyzed via qRT-PCR after treatment with methyl jasmonate, salicylic acid, abscisic acid, and ethylene. In the present study, we systematically identified and analyzed TaPKS gene family members in wheat, and our findings may facilitate the cloning of candidate genes associated with resistance to sheath blight in wheat