Advanced Geological Prediction

Due to the particularity of the tunnel project, it is difficult to find out the exact geological conditions of the tunnel body during the survey stage. Once it encounters unfavorable geological bodies such as faults, fracture zones, and karst, it will bring great challenges to the construction and will easily cause major problems, economic losses, and casualties. Therefore, it is necessary to carry out geological forecast work in the tunnel construction process, which is of great significance for tunnel safety construction and avoiding major disaster accident losses. This lecture mainly introduces the commonly used methods of geological forecast in tunnel construction, the design principles, and contents of geological forecast and combines typical cases to show the implementation process of comprehensive geological forecast. Finally, the development direction of geological forecast theory, method, and technology is carried out. Prospects provide a useful reference for promoting the development of geological forecast of tunnels

Rapid detection of porcine circovirus type 2 using a TaqMan-based real-time PCR

Porcine circovirus type 2 (PCV2) and the associated disease postweaning multisystemic wasting syndrome (PMWS) have caused heavy losses in global agriculture in recent decades. Rapid detection of PCV2 is very important for the effective prophylaxis and treatment of PMWS. To establish a sensitive, specific assay for the detection and quantitation of PCV2, we designed and synthesized specific primers and a probe in the open reading frame 2. The assay had a wide dynamic range with excellent linearity and reliable reproducibility, and detected between 102 and 1010 copies of the genomic DNA per reaction. The coefficient of variation for Ct values varied from 0.59% to 1.05% in the same assay and from 1.9% to 4.2% in 10 different assays. The assay did not cross-react with porcine circovirus type 1, porcine reproductive and respiratory, porcine epidemic diarrhea, transmissible gastroenteritis of pigs and rotavirus. The limits of detection and quantitation were 10 and 100 copies, respectively. Using the established real-time PCR system, 39 of the 40 samples we tested were detected as positive

Anomalous stopping of laser-accelerated intense proton beam in dense ionized matter

Ultrahigh-intensity lasers (10$^{18}$-10$^{22}$W/cm$^{2}$) have opened up new perspectives in many fields of research and application [1-5]. By irradiating a thin foil, an ultrahigh accelerating field (10$^{12}$ V/m) can be formed and multi-MeV ions with unprecedentedly high intensity (10$^{10}$A/cm$^2$) in short time scale ($\sim$ps) are produced [6-14]. Such beams provide new options in radiography [15], high-yield neutron sources [16], high-energy-density-matter generation [17], and ion fast ignition [18,19]. An accurate understanding of the nonlinear behavior of beam transport in matter is crucial for all these applications. We report here the first experimental evidence of anomalous stopping of a laser-generated high-current proton beam in well-characterized dense ionized matter. The observed stopping power is one order of magnitude higher than single-particle slowing-down theory predictions. We attribute this phenomenon to collective effects where the intense beam drives an decelerating electric field approaching 1GV/m in the dense ionized matter. This finding will have considerable impact on the future path to inertial fusion energy.Comment: 8 pages, 4 figure

Energy loss enhancement of very intense proton beams in dense matter due to the beam-density effect

Thoroughly understanding the transport and energy loss of intense ion beams in dense matter is essential for high-energy-density physics and inertial confinement fusion. Here, we report a stopping power experiment with a high-intensity laser-driven proton beam in cold, dense matter. The measured energy loss is one order of magnitude higher than the expectation of individual particle stopping models. We attribute this finding to the proximity of beam ions to each other, which is usually insignificant for relatively-low-current beams from classical accelerators. The ionization of the cold target by the intense ion beam is important for the stopping power calculation and has been considered using proper ionization cross section data. Final theoretical values agree well with the experimental results. Additionally, we extend the stopping power calculation for intense ion beams to plasma scenario based on Ohm's law. Both the proximity- and the Ohmic effect can enhance the energy loss of intense beams in dense matter, which are also summarized as the beam-density effect. This finding is useful for the stopping power estimation of intense beams and significant to fast ignition fusion driven by intense ion beams

The role of APOBEC3C in modulating the tumor microenvironment and stemness properties of glioma: evidence from pancancer analysis

BackgroundIt is now understood that APOBEC3 family proteins (A3s) are essential in tumor progression, yet their involvement in tumor immunity and stemness across diverse cancer types remains poorly understood.MethodsIn the present study, comprehensive genome-wide statistical and bioinformatic analyses were conducted to elucidate A3 family expression patterns, establishing clinically relevant correlations with prognosis, the tumor microenvironment(TME), immune infiltration, checkpoint blockade, and stemness across cancers. Different experimental techniques were applied, including RT–qPCR, immunohistochemistry, sphere formation assays, Transwell migration assays, and wound-healing assays, to investigate the impact of A3C on low-grade glioma (LGG) and glioblastoma multiforme (GBM), as well as its function in glioma stem cells(GSCs).ResultsDysregulated expression of A3s was observed in various human cancer tissues. The prognostic value of A3 expression differed across cancer types, with a link to particularly unfavorable outcomes in gliomas. A3s are associated with the the TME and stemness in multiple cancers. Additionally, we developed an independent prognostic model based on A3s expression, which may be an independent prognostic factor for OS in patients with glioma. Subsequent validation underscored a strong association between elevated A3C expression and adverse prognostic outcomes, higher tumor grades, and unfavorable histology in glioma. A potential connection between A3C and glioma progression was established. Notably, gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses implicated A3C in immune system-related diseases, with heightened A3C levels contributing to an immunosuppressive tumor microenvironment (TME) in glioma. Furthermore, in vitro experiments substantiated the role of A3C in sustaining and renewing glioma stem cells, as A3C deletion led to diminished proliferation, invasion, and migration of glioma cells.ConclusionThe A3 family exhibits heterogeneous expression across various cancer types, with its expression profile serving as a predictive marker for overall survival in glioma patients. A3C emerges as a regulator of glioma progression, exerting its influence through modulation of the tumor microenvironment and regulation of stemness

Target density effects on charge tansfer of laser-accelerated carbon ions in dense plasma

We report on charge state measurements of laser-accelerated carbon ions in the energy range of several MeV penetrating a dense partially ionized plasma. The plasma was generated by irradiation of a foam target with laser-induced hohlraum radiation in the soft X-ray regime. We used the tri-cellulose acetate (C$_{9}$H$_{16}$O$_{8}$) foam of 2 mg/cm$^{-3}$ density, and $1$-mm interaction length as target material. This kind of plasma is advantageous for high-precision measurements, due to good uniformity and long lifetime compared to the ion pulse length and the interaction duration. The plasma parameters were diagnosed to be T$_{e}$=17 eV and n$_{e}$=4 $\times$ 10$^{20}$ cm$^{-3}$. The average charge states passing through the plasma were observed to be higher than those predicted by the commonly-used semiempirical formula. Through solving the rate equations, we attribute the enhancement to the target density effects which will increase the ionization rates on one hand and reduce the electron capture rates on the other hand. In previsous measurement with partially ionized plasma from gas discharge and z-pinch to laser direct irradiation, no target density effects were ever demonstrated. For the first time, we were able to experimentally prove that target density effects start to play a significant role in plasma near the critical density of Nd-Glass laser radiation. The finding is important for heavy ion beam driven high energy density physics and fast ignitions.Comment: 7 pages, 4 figures, 35 conference

A novel approach for efficient Ni nanoparticle doping of MgB2 by liquid-assisted sintering

Ni nanoparticles were successfully introduced for preparing polycrystalline MgB2 samples by a novel reduction method. According to transmission electron microscopy, the obtained Ni nanoparticles with average grain size of 5 nm are distributed in the B matrix without agglomeration. The missing shift in X-ray diffraction peaks indicates that Ni nanoparticles could not substitute Mg sites in the lattice, but rather form the ternary compound MgNi 2.5B2 above 600 C. The low-melting eutectic liquid formed by MgNi at 506 C is responsible for the formation of plate-like MgB2 grains and the fast fabrication of MgB2 at low temperature. The sample doped with nano-Ni has better grain connectivity and the critical current density (Jc) with respect to the commercial Ni-doped sample, but there is no improvement compare with the pure MgB2 sample. A sintering model was accounted for understanding the reaction between Mg and B with the assistance of Ni nanoparticles. 2006 IEEE

Observation of flux jump in (MgB2)0.96Ni0.04 superconductor doped with milled Ni powders

Bulk (MgB 2) 0.96Ni 0.04 samples doping with premilled Ni powders were sintered at 750∈°C for 30 min. During sintering, liquid Mg-Ni phase prompts solid-solid reaction between Mg and B and the size of milled Ni powder determines the final distribution of the secondary MgNi 2.5B 2 phase in the sintered samples. A flux jump was observed in the (MgB 2) 0.96Ni 0.04 samples doping with Ni powders. Recognized from the measured superconductive properties, smaller-sized Ni powders can provide more effective flux pinning centers and thus improve the performance of the critical current density. 2011 Springer Science+Business Media, LLC

The improved superconductive properties of MgB2 bulks with minor Cu addition through reducing the MgO impurity

The minor Cu (\u3c3 \u3eat%) addition could significantly reduce the amount of MgO impurity in the in situ sintered MgB2 samples and thus improve the critical current density (Jc) at low field and the critical transition temperature (Tc). The formation of local Mg-Cu liquid at about 485 °C could wrap the neighboring Mg particles and thus protect them from the oxidation during the sintering process. Our results provide a possible route to further improve the superconductive properties of pure or doped MgB2 samples through decreasing the amount of MgO impurity with minor Cu addition. 2008 Elsevier B.V. All rights reserved