Complexation of Z-ligustilide with hydroxypropyl-β-cyclodextrin to improve stability and oral bioavailability

To improve the stability and oral bioavailability of Z-ligustilide (LIG), the inclusion complex of LIG with hydroxypropyl-β-cyclodextrin (HP-β-CD) was prepared by the kneading method and characterized by UV-Vis spectroscopy, differential thermal analysis (DTA) and Fourier transform infrared (FTIR) spectroscopy. LIG is capable of forming an inclusion complex with HP-β-CD and the stoichiometry of the complex was 1:1. Stability of the inclusion complex against temperature and light was greatly enhanced compared to that of free LIG. Further, oral bioavailability of LIG and the inclusion complex in rats were studied and the plasma drug concentration-time curves fitted well with the non-compartment model to estimate the absolute bioavailability, which was 7.5 and 35.9 %, respectively. In conclusion, these results show that LIG/HP-β-CD complexation can be of great use for increasing the stability and biological efficacy of LIG

Performance-Oriented Design for Intelligent Reflecting Surface Assisted Federated Learning

To efficiently exploit the massive amounts of raw data that are increasingly being generated in mobile edge networks, federated learning (FL) has emerged as a promising distributed learning technique. By collaboratively training a shared learning model on edge devices, raw data transmission and storage are replaced by the exchange of the local computed parameters/gradients in FL, which thus helps address latency and privacy issues. However, the number of resource blocks when using traditional orthogonal transmission strategies for FL linearly scales with the number of participating devices, which conflicts with the scarcity of communication resources. To tackle this issue, over-the-air computation (AirComp) has emerged recently which leverages the inherent superposition property of wireless channels to perform one-shot model aggregation. However, the aggregation accuracy in AirComp suffers from the unfavorable wireless propagation environment. In this paper, we consider the use of intelligent reflecting surfaces (IRSs) to mitigate this problem and improve FL performance with AirComp. Specifically, a performance-oriented design scheme that directly minimizes the optimality gap of the loss function is proposed to accelerate the convergence of AirComp-based FL. We first analyze the convergence behavior of the FL procedure with the absence of channel fading and noise. Based on the obtained optimality gap which characterizes the impact of channel fading and noise in different communication rounds on the ultimate performance of FL, we propose both online and offline approaches to tackle the resulting design problem. Simulation results demonstrate that such a performance-oriented design strategy can achieve higher test accuracy than the conventional isolated mean square error (MSE) minimization approach in FL.Comment: This work has been submitted to the IEEE for possible publicatio

Massive particles' Hawking radiation via tunneling from the G.H Dilaton black hole

In the past, Hawking radiation was viewed as a tunneling process and the barrier was just created by the outgoing particle itself. In this paper, Parikh's recent work is extended to the case of massive particles' tunneling. We investigate the behavior of the tunneling massive particles from a particular black hole solution-G.H Dilaton black hole which is obtained from the string theory, and calculate the emission rate at which massive particles tunnel across the event horizon. We obtain that the result is also consistent with an underlying unitary theory. Furthermore, the result takes the same functional form as that of massless particles.Comment: 6 pages, no figure, revtex

Intelligent Reflecting Surface Aided Multi-Tier Hybrid Computing

The Digital twin edge network (DITEN) aims to integrate mobile edge computing (MEC) and digital twin (DT) to provide real-time system configuration and flexible resource allocation for the sixth-generation network. This paper investigates an intelligent reflecting surface (IRS)-aided multi-tier hybrid computing system that can achieve mutual benefits for DT and MEC in the DITEN. For the first time, this paper presents the opportunity to realize the network-wide convergence of DT and MEC. In the considered system, specifically, over-the-air computation (AirComp) is employed to monitor the status of the DT system, while MEC is performed with the assistance of DT to provide low-latency computing services. Besides, the IRS is utilized to enhance signal transmission and mitigate interference among heterogeneous nodes. We propose a framework for designing the hybrid computing system, aiming to maximize the sum computation rate under communication and computation resources constraints. To tackle the non-convex optimization problem, alternative optimization and successive convex approximation techniques are leveraged to decouple variables and then transform the problem into a more tractable form. Simulation results verify the effectiveness of the proposed algorithm and demonstrate the IRS can significantly improve the system performance with appropriate phase shift configurations. Moreover, the results indicate that the DT assisted MEC system can precisely achieve the balance between local computing and task offloading since real-time system status can be obtained with the help of DT. This paper proposes the network-wide integration of DT and MEC, then demonstrates the necessity of DT for achieving an optimal performance in DITEN systems through analysis and numerical results

Massive uncharged and charged particles' tunneling from the Horowitz-Strominger Dilaton black hole

Originally, Parikh and Wilczek's work is only suitable for the massless particles' tunneling. But their work has been further extended to the cases of massive uncharged and charged particles' tunneling recently. In this paper, as a particular black hole solution, we apply this extended method to reconsider the tunneling effect of the H.S Dilaton black hole. We investigate the behavior of both massive uncharged and charged particles, and respectively calculate the emission rate at the event horizon. Our result shows that their emission rates are also consistent with the unitary theory. Moreover, comparing with the case of massless particles' tunneling, we find that this conclusion is independent of the kind of particles. And it is probably caused by the underlying relationship between this method and the laws of black hole thermodynamics.Comment: 6 pages, no figure, revtex 4, accepted by Int. J. Mod. Phys

Single-cell RNA sequencing reveals the transcriptomic characteristics of peripheral blood mononuclear cells in hepatitis B vaccine non-responders

The emergence of a vaccine against hepatitis B has proven to be an important milestone in the prevention of this disease; however, 5%–10% of vaccinated individuals do not generate an immune response to the vaccine, and its molecular mechanism has not been clarified. In this study, single-cell RNA sequencing was performed on peripheral blood mononuclear cells (PBMCs) from three volunteers with a high immune response (HR) and three with no immune response (NR) to the hepatitis B vaccine. We found that the antigen-presenting activity scores of various antigen-presenting cells, the mitogen-activated protein kinase (MAPK) pathway activity scores of naive B cells, and the cell activity scores of three types of effector T cells were significantly decreased, whereas the cytotoxicity scores of CD3highCD16lowKLRG1high natural killer T (NKT) cells were significantly increased in the NR group compared with those in the HR group. Additionally, the expression levels of some classical molecules associated with distinct signaling pathways—including HLA-B, HLA-DRB5, BLNK, BLK, IL4R, SCIMP, JUN, CEBPB, NDFIP1, and TXNIP—were significantly reduced in corresponding subsets of PBMCs from the NR group relative to those of the HR group. Furthermore, the expression of several cytotoxicity-related effector molecules, such as GNLY, NKG7, GZMB, GZMM, KLRC1, KLRD1, PRF1, CST7, and CTSW, was significantly higher in CD3highCD16lowKLRG1high NKT cells derived from non-responders. Our study provides a molecular basis for the lack of response to the hepatitis B vaccine, including defective antigen presentation, decreased T cell activity, and reduced IL-4 secretion, as well as novel insight into the role of NKT cells in the immune response to the hepatitis B vaccine

MALT1 regulates Th2 and Th17 differentiation via NF-κB and JNK pathways, as well as correlates with disease activity and treatment outcome in rheumatoid arthritis

ObjectiveMALT1 regulates immunity and inflammation in multiple ways, while its role in rheumatoid arthritis (RA) is obscure. This study aimed to investigate the relationship of MALT1 with disease features, treatment outcome, as well as its effect on Th1/2/17 cell differentiation and underlying molecule mechanism in RA.MethodsTotally 147 RA patients were enrolled. Then their blood Th1, Th2, and Th17 cells were detected by flow cytometry. Besides, PBMC MALT1 expression was detected before treatment (baseline), at week (W) 6, W12, and W24. PBMC MALT1 in 30 osteoarthritis patients and 30 health controls were also detected. Then, blood CD4+ T cells were isolated from RA patients, followed by MALT1 overexpression or knockdown lentivirus transfection and Th1/2/17 polarization assay. In addition, IMD 0354 (NF-κB antagonist) and SP600125 (JNK antagonist) were also added to treat CD4+ T cells.ResultsMALT1 was increased in RA patients compared to osteoarthritis patients and healthy controls. Meanwhile, MALT1 positively related to CRP, ESR, DAS28 score, Th17 cells, negatively linked with Th2 cells, but did not link with other features or Th1 cells in RA patients. Notably, MALT1 decreased longitudinally during treatment, whose decrement correlated with RA treatment outcome (treatment response, low disease activity, or disease remission). In addition, MALT1 overexpression promoted Th17 differentiation, inhibited Th2 differentiation, less affected Th1 differentiation, activated NF-κB and JNK pathways in RA CD4+ T cells; while MALT1 knockdown exhibited the opposite effect. Besides, IMD 0354 and SP600125 addition attenuated MALT1’s effect on Th2 and Th17 differentiation.ConclusionMALT1 regulates Th2 and Th17 differentiation via NF-κB and JNK pathways, as well as correlates with disease activity and treatment outcome in RA

Raffinose degradation-related gene GhAGAL3 was screened out responding to salinity stress through expression patterns of GhAGALs family genes

A-galactosidases (AGALs), the oligosaccharide (RFO) catabolic genes of the raffinose family, play crucial roles in plant growth and development and in adversity stress. They can break down the non-reducing terminal galactose residues of glycolipids and sugar chains. In this study, the whole genome of AGALs was analyzed. Bioinformatics analysis was conducted to analyze members of the AGAL family in Gossypium hirsutum, Gossypium arboreum, Gossypium barbadense, and Gossypium raimondii. Meanwhile, RT-qPCR was carried out to analyze the expression patterns of AGAL family members in different tissues of terrestrial cotton. It was found that a series of environmental factors stimulated the expression of the GhAGAL3 gene. The function of GhAGAL3 was verified through virus-induced gene silencing (VIGS). As a result, GhAGAL3 gene silencing resulted in milder wilting of seedlings than the controls, and a significant increase in the raffinose content in cotton, indicating that GhAGAL3 responded to NaCl stress. The increase in raffinose content improved the tolerance of cotton. Findings in this study lay an important foundation for further research on the role of the GhAGAL3 gene family in the molecular mechanism of abiotic stress resistance in cotton