7-[(5,5-Dimethyl-2-oxido-1,3,2-dioxaphosphinan-2-yl)­oxy]-4-methyl-2H-chromen-2-one

The title compound, C15H17O6P, was obtained from a reaction of 4-methyl-7-hy­droxy­coumarin and 2-chloro-5,5-dimethyl-1,3,2-dioxaphosphinane 2-oxide. There are two mol­ecules in the asymmetric unit in which the benzopyran ring system is almost planar [r.m.s. deviation for each molecule = 0.003 Å]. In the crystal, C—H⋯O hydrogen bonds and π–π stacking inter­actions [with centroid–centroid distances of 3.743 (3) and 3.727 (3) Å] link the two mol­ecules. The dioxaphospho­rinane ring adopts a chair conformation in both asymmetric molecules

5 GHz TMRT observations of 71 pulsars

We present integrated pulse profiles at 5~GHz for 71 pulsars, including eight millisecond pulsars (MSPs), obtained using the Shanghai Tian Ma Radio Telescope (TMRT). Mean flux densities and pulse widths are measured. For 19 normal pulsars and one MSP, these are the first detections at 5~GHz and for a further 19, including five MPSs, the profiles have a better signal-to-noise ratio than previous observations. Mean flux density spectra between 400~MHz and 9~GHz are presented for 27 pulsars and correlations of power-law spectral index are found with characteristic age, radio pseudo-luminosity and spin-down luminosity. Mode changing was detected in five pulsars. The separation between the main pulse and interpulse is shown to be frequency independent for six pulsars but a frequency dependence of the relative intensity of the main pulse and interpulse is found. The frequency dependence of component separations is investigated for 20 pulsars and three groups are found: in seven cases the separation between the outmost leading and trailing components decreases with frequency, roughly in agreement with radius-to-frequency mapping; in eleven cases the separation is nearly constant; in the remain two cases the separation between the outmost components increases with frequency. We obtain the correlations of pulse widths with pulsar period and estimate the core widths of 23 multi-component profiles and conal widths of 17 multi-component profiles at 5.0~GHz using Gaussian fitting and discuss the width-period relationship at 5~GHz compared with the results at at 1.0~GHz and 8.6~GHz.Comment: 46 pages, 14 figures, 8 Tables, accepted by Ap

The relationship between hepatic resistin overexpression and inflammation in patients with nonalcoholic steatohepatitis

BACKGROUND: The relationship between resistin and non-alcoholic steatohepatitis (NASH) is not clear, some studies claimed that serum resistin levels were associated with neither the presence of NASH nor its severity, others declared that serum resistin was related with inflammation and fibrosis in NASH. Our animal study verified that the distribution of resistin in the liver is correlated with inflammation in NASH. However, there is no pertinent study in humans. METHODS: Thirty patients with NASH, 28 simple steatosis, and 43 controls were recruited. Blood was collected for resistin, liver chemistries, fasting insulin and some metabolic parameters. Liver histology was scored according to NAFLD activity scoring system. Hepatic resistin expression was examined by real-time polymerase chain reaction, immunohistochemistry. Resistin protein expression was confirmed by western blotting in 13 patients with concomitant NAFLD and gallstone. RESULTS: Serum resistin was significantly elevated in both NASH and simple steatotic subjects compared with controls (all P < 0.05). Hepatic resistin was significantly increased in NASH patients in both mRNA and protein levels than those in simple steatosis and control subjects (all P < 0.05). Both serum and hepatic resistin had a correlation with obesity, but not with insulin resistance. The distribution of resistin positive cells was predominantly in perisinusoidal cells (such as Kupffer cells and hepatic stellate cells) in human NASH. Multivariate analysis revealed that waist-hip ratio, higher serum triglyceride, and hyperresistinemia were independent factors related to higher grade of steatosis; whereas hepatic resistin and serum cytokeratin predict NASH and severity of liver fibrosis. CONCLUSIONS: Hepatic resistin overexpression in NASH patients is associated with the severity of liver inflammation and fibrosis. Liver-derived resistin may be involved in the pathogenesis of human NASH

Bis(7-amino-2,4-dimethyl-1,8-naphthyridine)dinitratocadmium(II)

In the title compound, [Cd(NO3)2(C10H11N3)2], two naph­thyridine ring systems are coordinated to the Cd ion through the two N atoms in a bidentate chelating mode, whereas the remaining coordination sites are occupied by two O atoms from two different nitrate groups to complete the octahedral geometry. Inter­moleular N—H⋯O hydrogen bonds link the mol­ecules to form a one-dimensionnal sheet parallel to the ac plane. Weak slipped π–π stacking involving the naphthyridine ring systems stabilizes the structure

Decreased NPC1L1 expression in the liver from Chinese female gallstone patients

<p>Abstract</p> <p>Background</p> <p>Cholesterol gallstone disease is a very common disease in both industrialized and developing countries. Many studies have found that cholesterol gallstones are more common in women than men. The molecular mechanisms underlying the relationship between female gallstone disease and hepatic sterol transporters are still undergoing definition and have not been evaluated in humans.</p> <p>Aims</p> <p>The aim of this study is to probe for underlying hepatic molecular defects associated with development of gallstones in female.</p> <p>Methods/Results</p> <p>Fifty-seven nonobese, normolipidemic Chinese female gallstone patients (GS) were investigated with 12 age- and body mass index-matched female gallstone-free controls (GSF). The bile from the female GS had higher cholesterol saturation than that from the female GSF. The hepatic NPC1L1 mRNA levels were lower in female GS, correlated with SREBP2 mRNA. NPC1L1 downregulation was confirmed at protein levels. Consistently, immunohistochemistry showed decreased NPC1L1 expression in female GS.</p> <p>Conclusions</p> <p>The decreased hepatic NPC1L1 levels in female GS might indicate a downregulated reabsorption of biliary cholesterol in the liver, which, in turn, leads to the cholesterol supersaturation of bile. Our data are consistent with the possibility that hepatic NPC1L1 may be mediated by SREBP2.</p