A New Soil Sampling Design in Coastal Saline Region Using EM38 and VQT Method

Spatial sampling design based on the variability and distribution of soil properties is an important issue with the progress in precision agriculture and soil ecology. Electromagnetic induction (type EM38) and variance quad-tree (VQT) method were both applied to optimize the sampling scheme of soil salinity in a coastal reclamation field in north Jiangsu Province, China. Apparent soil electrical conductivity (ECa) measured with EM38 was used as an ancillary variable and the spatial distribution of ECa was used as priori information. The process and result of VQT algorithm analysis was illustrated and the obtained sampling strategy was validated using observed soil salinity. Then the spatial precision and sampling efficiency were evaluated. The result indicated that the spatial distribution of soil salinity produced with the VQT scheme was quite similar to that produced with total sampling sites, while sampling quantity of the former was reduced to approximately 1/2 of the latter. The spatial precision of VQT scheme was considerably higher than that of traditional grid method with respect to the same sampling number, and fewer samples were required for VQT scheme to obtain the same precision level. A 17.3% increase in sampling efficiency was achieved by VQT over grid method at the precision level of 90%. The VQT method was proved to be more efficient and economical because it can sample intensively or sparsely according to variation status in local areas. The associated application of EM38 and VQT method provides efficient tools and theoretical basis for saving sampling cost and improving sampling efficiency in coastal saline region and enriching soil ecology

Monitoring of deforestation events in the tropics using multidimensional features of Sentinel 1 radar data

Many countries and regions are currently developing new forest strategies to better address the challenges facing forest ecosystems. Timely and accurate monitoring of deforestation events is necessary to guide tropical forest management activities. Synthetic aperture radar (SAR) is less susceptible to weather conditions and plays an important role in high-frequency monitoring in cloudy regions. Currently, most SAR image-based deforestation identification uses manually supervised methods, which rely on high quality and sufficient samples. In this study, we aim to explore radar features that are sensitive to deforestation, focusing on developing a method (named 3DC) to automatically extract deforestation events using radar multidimensional features. First, we analyzed the effectiveness of radar backscatter intensity (BI), vegetation index (VI), and polarization feature (PF) in distinguishing deforestation areas from the background environment. Second, we selected the best-performing radar features to construct a multidimensional feature space model and used an unsupervised K-mean clustering method to identify deforestation areas. Finally, qualitative and quantitative methods were used to validate the performance of the proposed method. The results in Paraguay, Brazil, and Mexico showed that (1) the overall accuracy (OA) and F1 score (F1) of 3DC were 88.1–98.3% and 90.2–98.5%, respectively. (2) 3DC achieved similar accuracy to supervised methods without the need for samples. (3) 3DC matched well with Global Forest Change (GFC) maps and provided more detailed spatial information. Furthermore, we applied the 3DC to deforestation mapping in Paraguay and found that deforestation events occurred mainly in the second half of the year. To conclude, 3DC is a simple and efficient method for monitoring tropical deforestation events, which is expected to serve the restoration of forests after deforestation. This study is also valuable for the development and implementation of forest management policies in the tropics

Material Removal Mechanism and Force Model of Nanofluid Minimum Quantity Lubrication Grinding

Numerous researchers have developed theoretical and experimental approaches to force prediction in surface grinding under dry conditions. Nevertheless, the combined effect of material removal and plastic stacking on grinding force model has not been investigated. In addition, predominant lubricating conditions, such as flood, minimum quantity lubrication (MQL), and nanofluid minimum quantity lubrication (NMQL), have not been considered in existing force models. In this study, material removal mechanism under different lubricating conditions was researched. An improved theoretical force model that considers material removal and plastic stacking mechanisms was presented. Grain states, including cutting and ploughing, are determined by cutting efficiency (β). The influence of lubricating conditions was also considered in the proposed force model. Simulation was performed to obtain the cutting depth (a g) of each “dynamic active grain.” Parameter β was introduced to represent the plastic stacking rate and determine the force algorithms of each grain. The aggregate force was derived through the synthesis of each single-grain force. Finally, pilot experiments were conducted to test the theoretical model. Findings show that the model’s predictions were consistent with the experimental results, with average errors of 4.19% and 4.31% for the normal and tangential force components, respectively

Detection of mismatch repair gene germline mutation carrier among Chinese population with colorectal cancer

<p>Abstract</p> <p>Background</p> <p>Hereditary nonpolyposis colorectal cancer (HNPCC) is an autosomal dominant syndrome. The National Cancer Institute (NCI) has recommended the Revised Bethesda guidelines for screening HNPCC. There has been a great deal of research on the value of these tests in other countries. However, literature about the Chinese population is scarce. Our objective is to detect and study microsatellite instability (MSI) and mismatch repair (MMR) gene germline mutation carriers among a Chinese population with colorectal cancer.</p> <p>Methods</p> <p>In 146 prospectively recruited consecutive patients with clinically proven colorectal cancer, MSI carriers were identified by analysis of tumor tissue using multiplex fluorescence polymerase chain reaction (PCR) using the NCI recommended panel and classified into microsatellite instability-low (MSI-L), microsatellite instability-high (MSI-H) and microsatellite stable (MSS) groups. Immunohistochemical staining for MSH2, MSH6 and MLH1 on tissue microarrays (TMAs) was performed, and methylation of the MLH1 promoter was analyzed by quantitative methylation specific PCR (MSP). Germline mutation analysis of blood samples was performed for MSH2, MSH6 and MLH1 genes.</p> <p>Results</p> <p>Thirty-four out of the 146 colorectal cancers (CRCs, 23.2%) were MSI, including 19 MSI-H CRCs and 15 MSI-L CRCS. Negative staining for MSH2 was found in 8 CRCs, negative staining for MSH6 was found in 6 CRCs. One MSI-H CRC was negative for both MSH6 and MSH2. Seventeen CRCs stained negatively for MLH1. MLH1 promoter methylation was determined in 34 MSI CRCs. Hypermethylation of the MLH1 promoter occurred in 14 (73.7%) out of 19 MSI-H CRCs and 5 (33.3%) out of 15 MSI-L CRCs. Among the 34 MSI carriers and one MSS CRC with MLH1 negative staining, 8 had a MMR gene germline mutation, which accounted for 23.5% of all MSI colorectal cancers and 5.5% of all the colorectal cancers. Five patients harbored MSH2 germline mutations, and three patients harbored MSH6 germline mutations. None of the patients had an MLH1 mutation. Mutations were commonly located in exon 7 and 12 of MSH2 and exon 5 of MSH6. Right colonic lesions and mucinous carcinoma were not common in MSI carriers.</p> <p>Conclusion</p> <p>Our data may imply that the characteristics of HNPCC in the Chinese population are probably different from those of Western countries. Application of NCI recommended criteria may not be effective enough to identify Chinese HNPCC families. Further studies are necessary to echo or refute our results so as to make the NCI recommendation more universally applicable.</p

Optimal control for the multi-dimensional viscous Cahn-Hilliard equation

In this article, we study the multi-dimensional viscous Cahn-Hilliard equation. We prove the existence of optimal solutions and establish the optimality system

Induction of viable but nonculturable Escherichia coli O157:H7 by high pressure CO2 and its characteristics.

The viable but nonculturable (VBNC) state is a survival strategy adopted by many pathogens when exposed to harsh environmental stresses. In this study, we investigated for the first time that whether high pressure CO2 (HPCD), one of the nonthermal pasteurization techniques, can induce Escherichia coli O157:H7 into the VBNC state. By measuring plate counts, viable cell counts and total cell counts, E. coli O157:H7 in 0.85% NaCl solution (pH 7.0) was able to enter the VBNC state by HPCD treatment at 5 MPa and four temperatures (25°C, 31°C, 34°C and 37°C). Meanwhile, with the improvement of treatment temperature, the time required for E. coli O157:H7 to enter VBNC state would shorten. Enzymatic activities in these VBNC cells were lower than those in the exponential-phase cells by using API ZYM kit, which were also reduced with increasing the treatment temperature, but the mechanical resistance of the VBNC cells to sonication was enhanced. These results further confirmed VBNC state was a self-protection mechanism for some bacteria, which minimized cellular energetic requirements and increased the cell resistance. When incubated in tryptic soy broth at 37°C, the VBNC cells induced by HPCD treatment at 25°C, 31°C and 34°C achieved resuscitation, but their resuscitation capabilities decreased with increasing the treatment temperature. Furthermore, electron microscopy revealed changes in the morphology and interior structure of the VBNC cells and the resuscitated cells. These results demonstrated that HPCD could induce E. coli O157:H7 into the VBNC state. Therefore, it is necessary to detect if there exist VBNC microorganisms in HPCD-treated products by molecular-based methods for food safety