Preparation of Equine Immunoglobulin F(ab′) 2 against Smallpox and Evaluation of its Immunoprotective Effect

Smallpox, a severe infectious disease caused by the smallpox virus, causes a death rate as high as 30% within 15-20 days after infection. Therefore, development of anti-Smallpox product as a strategic reserve is urgently needed. We prepared and tested pepsin-digested F(ab′) 2 fragments of serum IgG from horses. Transmission electron microscopy indicated that the purified virus showed morphology consistent with VVTT. The titer was above 1.0 × 10 7 PFU/mL. The purity of the antigen exceeded 90%, according to HPLC. After purification and cleavage, the yield of the purified product F(ab′) 2 was approximately 1.3%, its purity exceeded 90%, and the neutralizing antibody titer exceeded 1:3200. F(ab′) 2 fragments had good preventive and therapeutic effects in mice at antibody doses of 5.2 mg/mL and 2.6 mg/mL. The viral loads of the drug-treated mice were suppressed to varying degrees, and the higher dose groups (5.2 and 2.6 mg/mL) showed a 2-3 fold lower viral load than that in the control group. A process for producing equine immunoglobulin F(ab′) 2 against VVTT was established. The prepared horse anti-smallpox immunoglobulin product had good neutralizing antibody effects on VVTT. The highly purified preparation may serve as a potential candidate for smallpox treatment

Recent Spatio-Temporal Variations of Suspended Sediment Concentrations in the Yangtze Estuary

Water and sediment are two of the most essential elements in estuaries. Their product, suspended sediment concentration (SSC), is involved in hydrology, geomorphology and ecology. This study was focused on the spatial and temporal variations of SSC in the Yangtze Estuary under new situations after the closure of ~50,000 dams in the Yangtze basin, including the Three Gorges Dam (TGD) in 2003. It was found that the SSC first exhibited an increasing and then a decreasing trend longitudinally from Xuliujing Station to the outer estuary with the Turbidity Maximum Zone located in the mouth bar area. Vertically, the SSC in the bottom layers averaged 0.96 kg/m3, about 2.4 times larger than the surface layers (0.40 kg/m3). During spring tides, the SSCs were always higher than those in neap tides, which was fit for the cognition law. As for the seasonal variations in the North Branch and mouth bar area, the SSCs in the dry season were higher than those in the flood season, while in the upper reach of the South Branch and outer estuary, the seasonal variation of SSCs reversed. This phenomenon primarily reflected the competition of riverine sediment flux and local resuspended sediment flux by wind-induced waves. As for the interannual changes, the SSCs demonstrated overall fluctuant downward trends, determined by riverine sediment flux and influenced by waves. This study revealed the new situation of SSC and can be a reference for other related researches in the Yangtze Estuary