Development of Probabilistic Corrosion Growth Models with Applications in Integrity Management of Pipelines

Metal-loss corrosion is a major threat to the structural integrity and safe operation of underground oil and gas pipelines worldwide. The reliability-based corrosion management program has been increasingly used in the pipeline industry, which typically includes three tasks, namely periodic high-resolution inline inspections (ILIs) to detect and size corrosion defects on a given pipeline, engineering critical assessment of the corrosion defects reported by the inspection tool and mitigation of defects. This study addresses the core involved in the reliability-based corrosion management program. First, the stochastic process in conjunction with the hierarchical Bayesian methodology is used to characterize the growth of defect depth using imperfect ILI data. The biases, random scattering errors as well as the correlations between the random scattering errors associated with the ILI tools are accounted for in the Bayesian inference. The Markov Chain Monte Carlo (MCMC) simulation techniques are employed to carry out the Bayesian updating and numerically evaluate the posterior distributions of the parameters in the growth model. Second, a simulation-based methodology is presented to evaluate the time-dependent system reliability of pressurized energy pipelines containing multiple active metal-loss corrosion defects using the developed growth models. Lastly, a probabilistic investigation is carried out to determine the optimal inspection interval for the newly-built onshore underground natural gas pipelines with respect to external metal-loss corrosion by considering the generation of corrosion defects over time and time-dependent growth of individual defects. The proposed methodology will facilitate the reliability-based corrosion management for corroding pipelines

The saturation number of K3,3K_{3,3}

A graph $G$ is called $F$-saturated if $G$ does not contain $F$ as a subgraph (not necessarily induced) but the addition of any missing edge to $G$ creates a copy of $F$. The saturation number of $F$, denoted by $sat(n,F)$, is the minimum number of edges in an $n$-vertex $F$-saturated graph. Determining the saturation number of complete partite graphs is one of the most important problems in the study of saturation number. The value of $sat(n,K_{2,2})$ was shown to be $\lfloor\frac{3n-5}{2}\rfloor$ by Ollmann, and a shorter proof was later given by Tuza. For $K_{2,3}$, there has been a series of study aiming to determine $sat(n,K_{2,3})$ over the years. This was finally achieved by Chen who confirmed a conjecture of Bohman, Fonoberova, and Pikhurko that $sat(n, K_{2,3})= 2n-3$ for all $n\geq 5$. In this paper, we prove a conjecture of Pikhurko and Schmitt that $sat(n, K_{3,3})=3n-9$ when $n \geq 9$

Hypoxic preacclimatization combining intermittent hypoxia exposure with physical exercise significantly promotes the tolerance to acute hypoxia

Background: Both hypoxia exposure and physical exercise before ascending have been proved to promote high altitude acclimatization, whether the combination of these two methods can bring about a better effect remains uncertain. Therefore, we designed this study to evaluate the effect of hypoxic preacclimatization combining intermittent hypoxia exposure (IHE) and physical exercise on the tolerance to acute hypoxia and screen the optimal preacclimatization scheme among the lowlanders.Methods: A total of 120 Han Chinese young men were enrolled and randomly assigned into four groups, including the control group and three experimental groups with hypoxic preacclimatization of 5-day rest, 5-day exercise, and 3-day exercise in a hypobaric chamber, respectively. Main physical parameters for hypoxia acclimatization, AMS incidence, physical and mental capacity were measured for each participant in the hypobaric chamber simulated to the altitude of 4500 m in the effect evaluation stage. The effect was compared between different schemes.Results: During the effect evaluation stage, SpO2 of the 5-day rest group and 5-day exercise group was significantly higher than that of the control group (p = 0.001 and p = 0.006, respectively). The participants with 5-day rest had significantly lower HR than the controls (p = 0.018). No significant differences of AMS incidence were found among the four groups, while the proportion of AMS headache symptom (moderate and severe vs. mild) was significantly lower in the 3-day exercise group than that in the control group (p = 0.002). The 5-day exercise group had significantly higher VO2max, than the other three groups (p = 0.033, p < 0.001, and p = 0.023, respectively). The 5-day exercise group also had significantly higher digital symbol and pursuit aiming test scores, while shorter color selection reaction time than the control group (p = 0.005, p = 0.005, and p = 0.004, respectively).Conclusion: Hypoxic preacclimatization combining IHE with physical exercise appears to be efficient in promoting the tolerance to acute hypoxia. Hypoxia duration and physical exercise of moderate intensity are helpful for improvement of SpO2 and HR, relief of AMS headache symptoms, and enhancement of mental and physical operation capacity

A secondary metabolite drives intraspecies antagonism in a gut symbiont that is inhibited by cell-wall acetylation

The mammalian microbiome encodes numerous secondary metabolite biosynthetic gene clusters; yet, their role in microbe-microbe interactions is unclear. Here, we characterized two polyketide synthase gene clusters (fun and pks) in the gut symbiont Limosilactobacillus reuteri. The pks, but not the fun, cluster encodes antimicrobial activity. Forty-one of 51 L. reuteri strains tested are sensitive to Pks products; this finding was independent of strains’ host origin. Sensitivity to Pks was also established in intraspecies competition experiments in gnotobiotic mice. Comparative genome analyses between Pks-resistant and -sensitive strains identified an acyltransferase gene (act) unique to Pks-resistant strains. Subsequent cell-wall analysis of wild-type and act mutant strains showed that Act acetylates cell-wall components, providing resistance to Pks-mediated killing. Additionally, pks mutants lost their competitive advantage, while act mutants lost their Pks resistance in in vivo competition assays. These findings provide insight into how closely related gut symbionts can compete and co-exist in the gastrointestinal tract