Effect of PAS-LuxR Family Regulators on the Secondary Metabolism of Streptomyces

With the development of sequencing technology and further scientific research, an increasing number of biosynthetic gene clusters associated with secondary Streptomyces metabolites have been identified and characterized. The encoded genes of a family of regulators designated as PAS-LuxR are gradually being discovered in some biosynthetic gene clusters of polyene macrolide, aminoglycoside, and amino acid analogues. PAS-LuxR family regulators affect secondary Streptomyces metabolites by interacting with other family regulators to regulate the transcription of the target genes in the gene cluster. This paper provides a review of the structure, function, regulatory mechanism, and application of these regulators to provide more information on the regulation of secondary metabolite biosynthesis in Streptomyces, and promote the application of PAS-LuxR family regulators in industrial breeding and other directions

Traffic Context Aware Data Augmentation for Rare Object Detection in Autonomous Driving

Detection of rare objects (e.g., traffic cones, traffic barrels and traffic warning triangles) is an important perception task to improve the safety of autonomous driving. Training of such models typically requires a large number of annotated data which is expensive and time consuming to obtain. To address the above problem, an emerging approach is to apply data augmentation to automatically generate cost-free training samples. In this work, we propose a systematic study on simple Copy-Paste data augmentation for rare object detection in autonomous driving. Specifically, local adaptive instance-level image transformation is introduced to generate realistic rare object masks from source domain to the target domain. Moreover, traffic scene context is utilized to guide the placement of masks of rare objects. To this end, our data augmentation generates training data with high quality and realistic characteristics by leveraging both local and global consistency. In addition, we build a new dataset, Rare Object Dataset (ROD), consisting 10k training images, 4k validation images and the corresponding labels with a diverse range of scenarios in autonomous driving. Experiments on ROD show that our method achieves promising results on rare object detection. We also present a thorough study to illustrate the effectiveness of our local-adaptive and global constraints based Copy-Paste data augmentation for rare object detection. The data, development kit and more information of ROD are available online at: \url{https://nullmax-vision.github.io}.Comment: The IEEE Conference on Robotics and Automation, ICRA 202

Spatial-Temporal Kinetic Behaviors of Micron-Nano Dust Adsorption along Epoxy Resin Insulator Surfaces and the Physical Mechanism of Induced Surface Flashover

The advanced Gas Insulated Switchgear/Gas Insulated Lines (GIS/GIL) transmission equipment serves as an essential physical infrastructure for establishing a new energy power system. An analysis spanning nearly a decade on faults arising from extra/ultra-high voltage discharges reveals that over 60% of such faults are attributed to the discharge of metal particles and dust. While existing technical means, such as ultra-high frequency and ultrasonic sensing, exhibit effectiveness in online monitoring of particles larger than sub-millimeter dimensions, the inherent randomness and elusive nature of micron-nano dust pose challenges for effective characterization through current technology. This elusive micron-nano dust, likely concealed as a latent threat, necessitates special attention due to its potential as a “safety killer”. To address the challenges associated with detecting micron-nano dust and comprehending its intricate mechanisms, this paper introduces a micron-nano dust adsorption experimental platform tailored for observation and practical application in GIS/GIL operations. The findings highlight that micron-nano dust’s adsorption state in the electric field predominantly involves agglomerative adsorption along the insulator surface and diffusive adsorption along the direction of the ground electrode. The pivotal factors influencing dust movement include the micron-nano dust’s initial position, mass, material composition, and applied voltage. Further elucidation emphasizes the potential of micron-nano dust as a concealed safety hazard. The study reveals specific physical phenomena during the adsorption process. Agglomerative adsorption results in micron-nano dust speckles forming on the epoxy resin insulator’s surface. With increasing voltage, these speckles undergo an “explosion”, forming an annular dust halo with deepening contours. This phenomenon, distinct from the initial adsorption, is considered a contributing factor to flashovers along the insulator’s surface. The physical mechanism behind flashovers triggered by micron-nano dust is uncovered, highlighting the formation of a localized short circuit area and intense electric field distortion constituted by dust speckles. These findings establish a theoretical foundation and technical support for enhancing the safe operational performance of AC and DC transmission pipelines’ insulation

Ancient cattle DNA provides novel insight into the subsistence mode transition from the late Neolithic to Bronze Age in the Nenjiang River Basin

The Honghe site (Heilongjiang Province, Northeast China) is located in the middle reaches of the Nenjiang River Basin, which situates rich cultural relics and faunal remains dating between the Neolithic and Bronze ages. The site hosts abundant bovine genus remains that are ideal for exploring the genetic history of the bovid species and the subsistence mode of the populations there. In this study, we recovered 14 mitochondrial genomes from 16 bovid samples selected for ancient DNA analysis. Phylogenetic analysis identified them as aurochs (Bos primigenius) and taurine cattle (Bos taurus), with one in eight Neolithic samples belonging to haplogroup T3, the most common type of domesticated cattle in ancient China. The remainder clustered with haplogroup C, found only in Chinese aurochs, while haplogroups C and T3 in six Bronze Age samples accounted for 50% each. Hence, it was speculated that domesticated cattle might have arisen in the middle reaches of the Nenjiang River Basin around 4000 years ago. However, the C-type aurochs remained among the essential resources for cattle production at the Angangxi people's disposal until the Bronze Age. Moreover, more T3-type taurine cattle were introduced during the Bronze Age. These domesticated cattle shared haplotypes with ancient cattle from the Northwest and Central Plains, showing close affinities, thus, reflecting frequent and extensive correlation between the middle reaches of the Nenjiang River and other region

High-Frequency Surface Insulation Strength with Nanoarchitectonics of Disiloxane Modified Polyimide Films

High-frequency power transformers are conducive to the reliable grid connection of distributed energy sources. Polyimide is often used for the coating insulation of high-frequency power transformers. However, creeping discharge will cause insulation failure, therefore, it is necessary to use disiloxane for the purpose of modifying the molecular structure of polyimide. This paper not only introduces 1,3-bis(3-aminopropyl)-1,1,3,3-tetramethyldisiloxane (GAPD) with a molar content of 1%, 2%, and 5% to polyimide, but also tests both the physical and chemical properties of the modified film and the high frequency creeping dielectric strength. The results show that after adding GAPD, the overall functional groups of the material do not change, at the same time the transfer complexation of intermolecular charge and the absorption of ultraviolet light increase. There is no phase separation of the material and the structure is more regular and ordered, moreover the crystallinity increases. The overall dielectric constant and the dielectric loss tangent value show different trends, which means that the former value increases, while the latter value decreases. In addition, the resistivity of the surface and the volume increase, which is the same as the glass transition temperature. The mechanical properties are excellent, and the strength of bulk breakdown is mounting. The insulation strength of the high frequency creeping surface has been improved, which will increase with larger contents of GAPD. Among them, the relative change of the creeping flashover voltage is not obvious, and the creeping discharge life of G5 is 4.77 times that of G0. Further analysis shows that the silicon-oxygen chain links of the modified film forms a uniformly dispersed Si-O-Si network in the matrix through chemical bonds and charge transfer complexation. Once the outer matrix is destroyed, it will produce dispersed flocculent inorganic particles which have the role of protecting the inner material and improving the performance of the material. Combined with the ultraviolet light energy absorption, the increase of deep traps, the reduction of dielectric loss, and the improvement of thermodynamic performance, can better improve the high-frequency creeping insulation strength of polyimide film and its potential application value

Adverse Effects on the Thyroid of Chinese Pregnant Women Exposed to Long-Term Iodine Excess: Optimal and Safe Tolerable Upper Intake Levels of Iodine

Ensuring optimal iodine nutrition in pregnant women is a global public health concern. However, there is no direct data on safe tolerable upper intake levels (ULs) for pregnant women. A cross-sectional study was performed to determine the ULs of pregnant women. A total of 744 pregnant women were enrolled in this study. The median (IQR) urinary iodine concentration (UIC) in pregnant women was 150.2 (87.6, 268.0) μg/L, and the urinary iodine excretion (UIE) over 24 h was 204.2 (116.0, 387.0) μg/day. Compared with those with a UIE figure of between 150–250 μg/day, the reference group, the prevalence of thyroid dysfunction was 5.7 times higher (95%CI: 1.7, 19.2) in pregnant women with a UIE figure of between 450–550 μg/day, and 3.9 times higher (95%CI: 1.5, 10.3) in pregnant women with a UIE figure of ≥550 μg/day. Compared with an estimated iodine intake (EII) of between 100–200 μg/day, the reference group, the prevalence of thyroid dysfunction was 4.3 times higher (95%CI: 1.3, 14.4) in pregnant women with a UIE figure of between 500–600 μg/day, and 3.6 times higher (95%CI: 1.5, 8.9) in pregnant women with UIE of ≥600 μg/day. In general, our cross-sectional study found that excessive iodine intake during pregnancy appears to directly increase the risk of thyroid dysfunction. Avoiding chronic iodine intakes of 500 μg/day or higher or having a UIE figure of ≥450 μg/day is recommended for pregnant women in China