A review on continuous-flow microfluidic PCR in droplets : advances, challenges and future

Significant advances have been made in developing microfluidic polymerase chain reaction (PCR) devices in the last two decades. More recently, microfluidic microdroplet technology has been exploited to perform PCR in droplets because of its unique features. For example, it can prevent crossover contamination and PCR inhibition, is suitable for single-cell and single molecule analyses, and has the potential for system integration and automation. This review will therefore focus on recent developments on droplet-based continuous-flow microfluidic PCR, and the major research challenges. This paper will also discuss a new way of on-chip flow control and a rational design simulation tool, which are required to underpin fully integrated and automated droplet-based microfluidic systems. We will conclude with a scientific speculation of future autonomous scientific discoveries enabled by microfluidic microdroplet technologies

Phase transitions in a holographic s+p model with backreaction

In a previous paper (arXiv:1309.2204, JHEP 1311 (2013) 087), we present a holographic s+p superconductor model with a scalar triplet charged under an SU(2) gauge field in the bulk. We also study the competition and coexistence of the s-wave and p-wave orders in the probe limit. In this work we continue to study the model by considering the full back-reaction The model shows a rich phase structure and various condensate behaviors such as the "n-type" and "u-type" ones, which are also known as reentrant phase transitions in condensed matter physics. The phase transitions to the p-wave phase or s+p coexisting phase become first order in strong back-reaction cases. In these first order phase transitions, the free energy curve always forms a swallow tail shape, in which the unstable s+p solution can also play an important role. The phase diagrams of this model are given in terms of the dimension of the scalar order and the temperature in the cases of eight different values of the back reaction parameter, which show that the region for the s+p coexisting phase is enlarged with a small or medium back reaction parameter, but is reduced in the strong back-reaction cases.Comment: 15 pages(two-column), 9 figure