Potential Role of Sugars in the Hyphosphere of Arbuscular Mycorrhizal Fungi to Enhance Organic Phosphorus Mobilization

Arbuscular mycorrhizal (AM) fungi engage in symbiosis with more than 80% of terrestrial plants, enlarging root phosphorus (P) absorption volume by producing extensive extraradical hyphae (ERH) in the soil. In addition, AM fungi recruit and cooperate with soil bacteria to enhance soil organic P mobilization and improve fungal and plant fitness through hyphal exudates. However, the role of the dominant compounds in the hyphal exudates in enhancing organic P mobilization in the mycorrhizal pathway is still not well understood. In this study, we added sugars, i.e., glucose, fructose, and trehalose, which are detected in the hyphal exudates, to the hyphal compartments (HCs) that allowed the ERH of the AM fungus to grow or not. The results showed that in AM fungus-inoculated pots, adding three sugars at a concentration of 2 mmol C kg-1 soil significantly increased the phosphatase activity and facilitated the mobilization of organic P in the HCs. The addition of fructose at a concentration of 2 mmol C kg-1 soil was the most efficient in increasing the phosphatase activity and enhancing organic P mobilization. The released inorganic P was then absorbed by the ERH of the AM fungus. The enhanced mobilization of organic P was correlated with the increase in phoD gene number and the changing bacterial community in the presence of fungal hyphae. The sugar addition enriched the relative abundance of some bacterial taxa, e.g., Betaproteobacteriales. Our study suggested that the addition of the sugars by mycorrhizae could be a pivotal strategy in managing P uptake in agricultural production, potentially directing future practices to optimize plant-fungi-bacteria interactions for improved P use efficiency.</p

Analysis on Safety Measures of Substation Maintenance

As an important node in the power grid, substation plays a very important role in the whole power grid. The equipment operation of the substation is carried out in order to timely and effectively detect the operation status of the equipment, find the latent fault of the equipment, and the maintenance of the substation is necessary for safe operation. However, there are some safety problems in the operation of the substation, which need to analyze the status quo of its safe operation and formulate the corresponding improvement measures. Based on this, this article on the substation maintenance work safety measures for a brief analysis, hoping to provide for future reference

A Combined Energy Method for Flutter Instability Analysis of Weakly Damped Panels in Supersonic Airflow

A combined energy method is proposed to investigate the flutter instability characteristics of weakly damped panels in the supersonic airflow. Based on the small damping assumption, the motion governing partial differential equation (PDE) of the panel aeroelastic system, is built by adopting the first-order piston theory and von Karman large deflection plate theory. Then by applying the Galerkin procedure, the PDE is discretized into a set of coupled ordinary differential equations, and the system reduced order model (ROM) with two degrees of freedom is obtained. Considering that the panel aeroelastic system is non-conservative in the physical nature, and assuming that the panel exhibits a single period oscillation on the flutter occurrence, the non-conservative energy balance principle is applied to the linearized ROM within one single oscillation period. The obtained result shows that the ROM modal coordinate amplitudes ratio is regulated by the modal damping coefficients ratio, though each modal damping coefficient is small. Furthermore, as the total damping dissipation energy can be eliminated due to its smallness, the He’s energy balance method is applied to the undamped ROM, therefore the critical non-dimensional dynamic pressure on the flutter instability occurrence, and the oscillation circular frequency amplitude relationship (linear and nonlinear form) are derived. In addition, the damping destabilization paradoxical influence on the system flutter instability is investigated. The accuracy and efficiency of the proposed method are validated by comparing the results with that obtained by using Routh Hurwitz criteria

Arbitrarily shaped video object coding by wavelet

Video object coding is one of the most important functionalities proposed by MPEG-4. In this paper, we propose a new wavelet method to encode the texture of an arbitrarily shaped object, both for the still and for the video object. The method uses the shape adaptive wavelet transform (SA-DWT) in MPEG-4 still object coding, but with a computationally more efficient lifting im-plementation. The transformed object coefficients are then quantized and entropy encoded with a partial bit-plane embedded coder, which greatly improves the coding efficiency. We denote the coding algorithm as the video object wavelet (VOW) coder. Experimental results show that VOW significantly outperforms MPEG-4 in still object coding, and achieves a comparable per-formance in video object coding in terms of PSNR. Moreover, the VOW decoded object looks better subjectively, with less annoying blocking artifacts than that of MPEG-4. ∗ This work was done while Dr. Guiwei Xing was an intern at Microsoft Research China

Arbitrarily Shaped Video Object Coding by Wavelet

Video object coding is one of the most important functionalities proposed by MPEG4. In this paper, we propose a new wavelet method to encode the texture of an arbitrarily shaped object, both for the still and for video object. The method uses the shape adaptive wavelet transform (SA-DWT) in MPEG4 still object coding, but with a computationally more efficient lifting implementation

Identification and Expression Analysis of <i>Wnt2</i> Gene in the Sex Differentiation of the Chinese Soft-Shelled Turtle (<i>Pelodiscus sinensis</i>)

The Chinese soft-shelled turtle (Pelodiscus sinensis) is an important freshwater aquaculture animal in China. The Wnt gene family plays important regulatory roles in the development and growth of mammals. However, the precise function of these family genes has not been well understood in the sex differentiation of Chinese soft-shelled turtles. Here, we cloned a member of the Wnt family, Wnt2, which obtained a 1077 bp open reading frame that encoded a 358-aa protein. The putative amino acid sequences of proteins are exceeded 80% identical to other turtles. The expression level of Wnt2 peaked at the 14th stage both in female and male embryos during the early gonadal differentiation period of Chinese soft-shelled turtles, which occurred before gonadal differentiation. Wnt2 mRNA was expressed at higher levels in the brains and gonads of mature P. sinensis females compared with those in mature males. Wnt agonists significantly affected the expression level of Wnt2 during the gonadal differentiation period. After Wnt agonists (1.0 μg/μL, 2.5 μg/μL, 5.0 μg/μL) treatment, the expression level of the Wnt2 generally appeared to have an inverted-V trend over time in female embryonic gonads. The results suggested that Wnt2 may participate in the regulation of gonad development in P. sinensis during the early embryonic stages. These results could provide a theoretical basis for the reproduction process of the Chinese soft-shelled turtle

Influence of Layer Thickness and Raster Angle on the Mechanical Properties of 3D-Printed PEEK and a Comparative Mechanical Study between PEEK and ABS

Fused deposition modeling (FDM) is a rapidly growing 3D printing technology. However, printing materials are restricted to acrylonitrile butadiene styrene (ABS) or poly (lactic acid) (PLA) in most Fused deposition modeling (FDM) equipment. Here, we report on a new high-performance printing material, polyether-ether-ketone (PEEK), which could surmount these shortcomings. This paper is devoted to studying the influence of layer thickness and raster angle on the mechanical properties of 3D-printed PEEK. Samples with three different layer thicknesses (200, 300 and 400 μm) and raster angles (0°, 30° and 45°) were built using a polyether-ether-ketone (PEEK) 3D printing system and their tensile, compressive and bending strengths were tested. The optimal mechanical properties of polyether-ether-ketone (PEEK) samples were found at a layer thickness of 300 μm and a raster angle of 0°. To evaluate the printing performance of polyether-ether-ketone (PEEK) samples, a comparison was made between the mechanical properties of 3D-printed polyether-ether-ketone (PEEK) and acrylonitrile butadiene styrene (ABS) parts. The results suggest that the average tensile strengths of polyether-ether-ketone (PEEK) parts were 108% higher than those for acrylonitrile butadiene styrene (ABS), and compressive strengths were 114% and bending strengths were 115%. However, the modulus of elasticity for both materials was similar. These results indicate that the mechanical properties of 3D-printed polyether-ether-ketone (PEEK) are superior to 3D-printed ABS

Precision Security: Integrating Video Surveillance with Surrounding Environment Changes

Video surveillance plays a vital role in maintaining the social security although, until now, large uncertainty still exists in danger understanding and recognition, which can be partly attributed to intractable environment changes in the backgrounds. This article presents a brain-inspired computing of attention value of surrounding environment changes (EC) with a processes-based cognition model by introducing a ratio value λ of EC-implications within considered periods. Theoretical models for computation of warning level of EC-implications to the universal video recognition efficiency (quantified as time cost of implication-ratio variations from λk to λk+1, k=1,2,…) are further established. Imbedding proposed models into the online algorithms is suggested as a future research priority towards precision security for critical applications and, furthermore, schemes for a practical implementation of such integration are also preliminarily discussed

Radial Compressive Property and the Proof-of-Concept Study for Realizing Self-expansion of 3D Printing Polylactic Acid Vascular Stents with Negative Poisson’s Ratio Structure

Biodegradable stents offer the potential to reduce the in-stent restenosis by providing support long enough for the vessel to heal. The polylactic acid (PLA) vascular stents with negative Poisson&rsquo;s ratio (NPR) structure were manufactured by fused deposition modeling (FDM) 3D printing in this study. The effects of stent diameter, wall thickness and geometric parameters of arrowhead NPR structure on radial compressive property of 3D-printed PLA vascular stent were studied. The results showed that the decrease of stent diameter, the increase of wall thickness and the increase of the surface coverage could enhance the radial force (per unit length) of PLA stent. The radial and longitudinal size of PLA stent with NPR structure decreased simultaneously when the stent was crimped under deformation temperature. The PLA stent could expand in both radial and longitudinal direction under recovery temperature. When the deformation temperature and recovery temperature were both 65 &deg;C, the diameter recovery ratio of stent was more than 95% and the maximum could reach 98%. The length recovery ratio was above 97%. This indicated the feasibility of utilizing the shape memory effect (SME) of PLA to realize the expansion of 3D-printed PLA vascular stent under temperature excitation