Dual-rail CMOS circuits

Dual-rail CMOS logic, also called as differential CMOS logic handles both true and complementary signals at any time. Various dual-rail CMOS logic styles have been designed and explored, including differential cascade voltage switch logic (DCVSL), complementary pass-transistor logic (CPL), differential cascade voltage switch with pass-gate (DCVSPG) logic, and double pass-transistor logic (DPL). These kinds of dual-rail CMOS logic will be simulated by Cadence. The simulation results and application of the dual-rail CMOS circuits in full adder are also presented in this dissertation, which will be tested by simulating circuits with Cadence.Master of Science (Electronics

Low-noise sensor interface circuits for implantable brain interfaces

The technological of low -noise sensor interface circuits is a frontier science in nowadays.If the research of this kind of circuit is successful,the circuit will be widely used in medicine,for example,the implantable brain interfaces.In this report,an new kind of neural amplifier will be designed and it uses a MOS-bipolar pseudo-resistor element to form this circuit.Certainly,this amplifier can amplify low-frequency signals with low-noise.This amplifier is fully integrated with characteristics like low power consumption and small area,and it can record a large range frequency of biological signal. In this circuit of amplifier, the capacitance will be used to reject dc offsets and pass the low-frequency signals.The amplification will be C1/C2,it is setted to 40dB and it can be implemented by the simulation test.This circuit also be proved that it has an input-referred noise of 4.822 uVrms.We can see in this paper that this amplifier can balance the noise and power.Bachelor of Engineerin

MicroRNA-384 Inhibits the Progression of Papillary Thyroid Cancer by Targeting PRKACB

Background. Growing evidence shows that dysregulation of miRNAs plays a significant role in papillary thyroid cancer (PTC) tumorigenesis and development. The abnormal expression of miR-384 has been acknowledged in the proliferation or metastasis of some cancers. However, the function and the underlying mechanism of miR-384 in PTC progression remain largely unknown. Methods. Real-time PCR was conducted to detect miR-384 expression in 58 cases of PTC and their adjacent noncancerous tissues. MTT, soft agar assay Transwell assay, and wound-healing assay were carried out to explore the biological function of miR-384 in PTC cell lines of BCPAP and K1. Bioinformatics analysis, dual-luciferase reporter assay, western blot, and functional complementation analysis were conducted to explore the target gene of miR-384. Moreover, Spearman’s correlation analysis was conducted to reveal the correlation between miR-384 and PRKACB mRNA in PTC. Results. The expression of miR-384 decreased obviously in PTC, especially in the tumors with lymph node metastasis or larger tumor size. The ectopic upregulation of miR-384 significantly suppressed PTC progression, and the inhibition of miR-384 had the opposite effects. Moreover, PRKACB gene was confirmed as the target of miR-384. Conclusion. The study suggests that miR-384 serves as a tumor suppressor in PTC progression by directly targeting the 3′-UTR of PRKACB gene

Diversity and traditional knowledge of medicinal plants used by Shui people in Southwest China

Abstract Background The Shui are a small Chinese sociolinguistic group living in Sandu Shui Autonomous County, south of Guizhou Province. The Shui people have accumulated and developed rich traditional medicinal knowledge, which has played a significant role in their healthcare. Traditional ethnic herbal medicines, like Shui ethnomedicine, have become an important resource of rural development in Guizhou Province. However, not much research has been conducted to document the medicinal plants traditionally used by the Shui people. This study’s aims are (1) to record the current use of medicinal plants in Sandu County and associated traditional knowledge, including the medicinal plant species used and the types of diseases treated by local healers and any unique aspects of their preparations; (2) to analyze the most important medicinal plant species using relative frequency of citation (RFC); and (3) to provide useful information and data for possible future development and application of ethnomedicine and promote the conservation and preservation of Shui traditional medicinal knowledge. Methods Field surveys were conducted between July 2015 and August 2022 in Sandu County. A total of 15 local healers as key informants were interviewed. An additional 132 informants from villages and local markets were also interviewed through semistructured interviews and focal group discussions. Local Shui healers were followed during their collection of medicinal plants in the fields and recorded the medicinal plants’ names, uses, and parts used. An ANOVA was used to evaluate the number of medicinal plants recognized by local healers across age-groups and townships, and relative frequencies of citation values were determined for the recorded medicinal plants. Results In this study, data collected from 15 Shui healers and 132 other informants were analyzed. The healers used fresh or dried parts of 505 plant species as medicine to treat a wide range of conditions and diseases. These plants belong to 405 genera from 156 families, with Fabaceae being the highest represented plant family. The Jiuqian township had the highest distribution of per capita healers (pch); only one local healer was in Zhonghe. Of the 15 local healers, only two were younger than 40 years of age. There is a major concern that traditional medicinal knowledge may be lost if there are not sufficient trainees or suitable successors. Among the common medicinal plants, most are herbaceous and the Shui typically use the whole plant in their medicines. There are 85 different recorded diseases treated by Shui medicinal plants, and among them, rheumatism and bone fractures have the largest number of species used. Three medicinal plant species, Isodon amethystoides, Asarum insigne, and Acorus tatarinowii, are the most commonly used ethnomedicines by the Shui people. Conclusion This study demonstrated that Shui people have extensive knowledge of a diverse range of medicinal plants, many of which had not been systematically recorded before the current study. Further research on the chemistry, pharmacology, and toxicity of Shui medicinal plants will be useful for developing functional foods or pharmaceutical products, particularly those of Isodon amethystoides, Asarum insigne, and Acorus tatarinowii. Additionally, as a result of rapid economic growth, fewer young people in Shui communities pursue traditional medicinal studies. Only 15 traditional Shui healers remain in the county, and only two of them are below the age of 40 years. Therefore, to conserve Shui’s traditional medicinal knowledge, initiatives and policies are required to regenerate, strengthen, and promote Shui medicinal knowledge

Real-Time LEO Satellite Clocks Based on Near-Real-Time Clock Determination with Ultra-Short-Term Prediction

The utilization of Low Earth Orbit (LEO) satellites is anticipated to augment various aspects of traditional GNSS-based Positioning, Navigation, and Timing (PNT) services. While the LEO satellite orbital products can nowadays be produced with rather high accuracy in real-time of a few centimeters, the precision of the LEO satellite clock products that can be achieved in real-time is less studied. The latter, however, plays an essential role in the LEO-augmented positioning and timing performances. In real-time, the users eventually use the predicted LEO satellite clocks, with their precision determined by both the near-real-time clock precision and the prediction time needed to match the time window for real-time applications, i.e., the precision loss during the prediction phase. In this study, a real-time LEO satellite clock determination method, consisting of near-real-time clock determination with ultra-short-term clock prediction is proposed and implemented. The principles and strategies of this method are discussed in detail. The proposed method utilized Kalman-filter-based processing, but supports restarts at pre-defined times, thus hampering continuous bias propagation and accumulation from ancient epochs. Based on the method, using Sentinel-3B GNSS observations and the real-time GNSS products from the National Center for Space Studies (CNES) in France, the near-real-time LEO satellite clocks can reach a precision of 0.2 to 0.3 ns, and the precision loss during the prediction phase is within 0.07 ns for a prediction time window from 30 to 90 s. This results in a total error budget in the real-time LEO satellite clocks of about 0.3 ns

Yao herbal medicinal market during the Dragon Boat Festival in Jianghua County, China

Abstract Background The traditional medicinal markets held during the Dragon Boat Festival are common and important in China’s countryside. In Jianghua, a Yao autonomous county in Hunan Province in China, the medicinal market also plays an important role for the application, conservation, and communication of traditional Yao medicinal knowledge. Methods During the Dragon Boat Festival in 2016 and 2017, ethnobotanical surveys and inventories were conducted in the medicinal market of Jianghua County, and voucher plant specimens were collected, identified, and deposited in a herbarium. Quantitative analysis included measurement of frequency of occurrence for species in the marketplace and the relative importance index for the number of uses for a given species. Results A total of 306 plant species (249 genera, 113 families) and their related information about the medicinal market were collected. Some major findings include the following: (1) Using the whole plant as medicine is more common than other medicinal plant parts; (2) treating rheumatism and clearing inner heat are the most frequent medicinal uses; and (3) taking a medicinal bath is the most frequent modality to administer the traditional medicine. The frequency of occurrence and the relative importance index of some medicinal plants were analyzed, as well as the demographics and the number of stalls and the status of traditional Yao medicinal knowledge in Jianghua. Based on the investigation, suggestions were proposed for better protecting the medicinal market and preserving traditional medicinal knowledge in Jianghua County. Conclusion The medicinal market during the Dragon Boat Festival in Jianghua County possesses an important cultural value and helps to conserve the traditional Yao medicinal knowledge. The medicinal plants sold at the market showed great diversity and unique local characteristics. The medicinal market is facing some challenges in such a rapidly developing era. Cultivation of young healers and maintaining the local biodiversity might be the key solutions for the development of local medicinal market and local Yao medicinal knowledge

Clonal fitness inferred from time-series modelling of single-cell cancer genomes

Progress in defining genomic fitness landscapes in cancer, especially those defined by copy number alterations (CNAs), has been impeded by lack of time-series single-cell sampling of polyclonal populations and temporal statistical models1-7. Here we generated 42,000 genomes from multi-year time-series single-cell whole-genome sequencing of breast epithelium and primary triple-negative breast cancer (TNBC) patient-derived xenografts (PDXs), revealing the nature of CNA-defined clonal fitness dynamics induced by TP53 mutation and cisplatin chemotherapy. Using a new Wright-Fisher population genetics model8,9 to infer clonal fitness, we found that TP53 mutation alters the fitness landscape, reproducibly distributing fitness over a larger number of clones associated with distinct CNAs. Furthermore, in TNBC PDX models with mutated TP53, inferred fitness coefficients from CNA-based genotypes accurately forecast experimentally enforced clonal competition dynamics. Drug treatment in three long-term serially passaged TNBC PDXs resulted in cisplatin-resistant clones emerging from low-fitness phylogenetic lineages in the untreated setting. Conversely, high-fitness clones from treatment-naive controls were eradicated, signalling an inversion of the fitness landscape. Finally, upon release of drug, selection pressure dynamics were reversed, indicating a fitness cost of treatment resistance. Together, our findings define clonal fitness linked to both CNA and therapeutic resistance in polyclonal tumours.ISSN:0028-0836ISSN:1476-468