Anti-Hepatitis E Antibody in Hemodialysis Patients

WOS: 00042516340002

Comparison of immunofluorescence assay and multiplexed microparticle-based immunoassay for detecting

WOS: 000254720500042PubMed ID: 18215427A new multiplexed microparticle-based immunoassay was compared with the immunofluorescence assay that is used widely for detecting EBV-specific antibodies in immunocompetent patients. Serum samples of 162 patients submitted for routine EBV diagnosis were tested for viral capsid antigen IgM, viral capsid antigen IgG and serological profile interpretations with both systems. The result concordances were 94.2%, 93.6%, and 92.1%, respectively. Multiplexed microparticle-based immunoassay can be an alternative to immunofluorescence assay especially in laboratories receiving large numbers of samples. (C) 2007 Elsevier B.V. All rights reserved

Comparison of Two Anti-HIV Confirmatory Assays: Recombinant HIV 1/2 Line Immunoassay and Geenius HIV 1/2 Confirmatory Test

WOS: 000582397200009PubMed: 33107290The main purpose in the diagnosis of human immunodeficiency virus (HIV) infection is to rapidly and accurately identify people with HIV infection. It is recommended that samples that are repeatedly reactive should be verified/supported according to the classical algorithms of international and national guidelines. the recombinant "line immunoassay test (LIA)", which has been used for many years, is studied with the accumulated samples to be cost and labor-effective. in this study, the supplemental recombinant HIV 1/2 LIA (INNO-LIA (R), Fujirebio, Ghent, Belgium) used to confirm and differentiate the diagnosis of HIV-1 and HIV-2 infections, and an immunochromatographic supplemental test (Geenius (TM) (Bio-Rad Laboratories, Marnes-la-Coquette, France) which can provide faster results were compared. One hundred fifty serum samples sent to Ege University Faculty of Medicine Hospital Medical Virology Laboratory with anti-HIV 1/2 and p24 antigen positive and indeterminant results and three HIV-1 positive external quality control samples were included in the study. Samples were tested both with the Geenius (TM) HIV 1/2 (Bio-Rad Laboratories, Marnes-la-Coquette, France) and recombinant HIV 1/2 LIA (INNO-LIA (R), Fujirebio, Ghent, Belgium). HIV 1 viral load was evaluated by using Abbott real-time HIV-1 test in Abbott m200sp system (Abbott Molecular, Wiesbaden, Germany) in plasma samples. in both assays, the results were consistent in 147 samples (96.08%). Six samples that have discordant results were as follows: one sample was LIA HIV-1 positive and Geenius indeterminate, two samples were LIA indeterminant and Geenius HIV-1 positive, and in three samples, LIA was indeterminate and Geenius negative. in two EIA reactive samples (2/97, 2.06%) and three EIA negative samples (3/53, 5.66%) LIA results were indeterminant. Geenius test, on the other hand, correctly identified HIV positive and negative samples. the immunochromatographic test could be used in the diagnostic algorithm of HIV infection, due to its short application time, not being labor intensive, its ability to distinguish HIV-1/2, its high sensitivity/specificity compared to LIA, and the compliance with LIA. However, it should be noted that in acute HIV infection, all analytical antibody tests, become reactive later than the fourth generation enzyme immunoassays

Cytomegalovirus Disease Causing Outbreak in Family Members

WOS: 000488951800014Primary cytomegalovirus (CMV) infection generally occurs during childhood/early adolescence and is usually an asymptomatic or mild and self-limiting disease in immunocompetent patients. Mononucleosis-like syndrome due to primary CMV may occur in middle-aged or advanced-aged adults, though rare. Three middle-aged patients from the same family admitted to our clinic with acute CMV infection. Two of the three cases were hospitalized, CMV antibodies were examined and at the same time the third case had an acute CMV infection. CMV transmission among middle-aged family members Is very rare. This study reports three cases of acute CMV infection and it's transmission from person to person in the same family

Evaluation of anti-HCV Line immunoassay indeterminant results

19th Annual Meeting of the European-Society-for-Clinical-Virology -- SEP 14-17, 2016 -- Lisbon, PORTUGALWOS: 000390584800131European Soc Clin Viro